Purification and Characterization of a Phage-encoded Cytotoxin from an Escherichia coli O111 Strain Associated with Hemolytic-uremic Syndrome

Molecular cloning and nucleotide sequence of another variant of the Escherichia coli Shiga-like toxin II family

“…Induction of bacteriophages was accomplished by: (1) exposure of cultures to ultraviolet light as described by O'Brien ef al. (1984); and (2) addition of mitomycin C (Sigma) to midexponential-phase cultures (ODbo0 0.5) to a final concentration of 1 pg ml-I (Karch & Bitzman, 1988).…”

Evaluation of oligonucleotide probes for identification of shiga-like-toxin-producing Escherichia coli

“…Cytotoxicity assays. The assay used to quantitate the amount of toxin was performed as described recently (14), with minor modifications. Briefly, Vero cells (ATCC CCL 81; Flow Laboratories, Meckenheim, Federal Republic of Germany) or HeLa-S3 cells (ATCC CCL2.2; Flow Laboratories) were grown as monolayers in minimal essential medium supplemented with L-glutamine, antibiotics (penicillin and streptomycin), and 3% fetal bovine serum.…”

“…SLT-I from E. coli C600 lysogenized with coliphage 933J (41) of E. coli serotype 0157:H7 strain 933 and SLT-II from E. coli C600 lysogenized with phage 933W (41) of E. coli 933 have been purified in our laboratory (17). Other Shiga-like cytotoxins used were the SLT-I from E. coli HUS-2, Hamburg (E. coli serotype 0111:H-) (14). Shiga toxin was purified from S. dysenteriae type 1 strain S31 (14).…”

“…Other Shiga-like cytotoxins used were the SLT-I from E. coli HUS-2, Hamburg (E. coli serotype 0111:H-) (14). Shiga toxin was purified from S. dysenteriae type 1 strain S31 (14). The respective antitoxins have been raised in rabbits (14,17).…”

“…Shiga toxin was purified from S. dysenteriae type 1 strain S31 (14). The respective antitoxins have been raised in rabbits (14,17). MAb 13C4 against the B subunit of SLT-I (40) was a kind gift from N. A. Strockbine (Bethesda, Md.…”