Detection of several clostridia by a direct fluorescent antibody test in formalin-fixed, paraffin-embedded tissues

Assis, Ronnie Antunes de; Lobato, Francisco Carlos Faria; Salvarani, Felipe Masiero; Lima, C.; Uzal, Francisco A.

doi:10.1590/s0102-09352007000500033

Cited by 4 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7,8 FAT is faster and less expensive than IHC, and it has the advantage that it can be performed on smears or on tissues fixed in formalin for short periods of time. 1,8 IHC is particularly useful for retrospective studies by detecting the organism within lesions. 7 PCR is a very useful method for the identification of C. chauvoei in clinical samples, 9 pure cultures, or in formalinfixed, paraffin-embedded (FFPE) tissues.…”

Section: Diagnosismentioning

confidence: 99%

“…The identification of the bacteria can be accomplished through anaerobic culture, immunodetection by a fluorescent antibody test (FAT) or immunohistochemistry (IHC), and/or PCR. 1,3,7,8,12,63 Microbial isolation is not always possible because of the difficulties in submitting samples to the laboratory, and also because not all diagnostic laboratories around the world have facilities available for anaerobic culture. 3,63 In addition, postmortem contaminants, including other fast-growing anaerobes such as C. septicum , may interfere with the isolation of C. chauvoei .…”

Section: Diagnosismentioning

confidence: 99%

“…58 Both methods are simple, rapid, and have similar sensitivity and specificity. 7,8 FAT is faster and less expensive than IHC, and it has the advantage that it can be performed on smears or on tissues fixed in formalin for short periods of time. 1,8 IHC is particularly useful for retrospective studies by detecting the organism within lesions.…”

Section: Diagnosismentioning

confidence: 99%

See 2 more Smart Citations

A review of cardiac blackleg in cattle, and report of 2 cases without skeletal muscle involvement in Argentina

et al. 2022

View full text Add to dashboard Cite

Blackleg is an infectious disease caused by Clostridium chauvoei. Cardiac blackleg has been reported in ruminants as an uncommon presentation of the disease; its pathogenesis is not understood completely. We include here a literature review of cardiac blackleg and a description of 2 cases in 12–15-mo-old feedlot steers in Argentina. Fourteen of 1,190 steers died suddenly over a period of 10 d. Postmortem examinations were performed on 5 of these animals. Grossly, severe, diffuse, fibrinous pericarditis and pleuritis, multifocal necrohemorrhagic myocarditis, diffuse pulmonary congestion, mild splenomegaly, and moderate congestion of meningeal vessels were observed. No significant gross lesions were observed in the skeletal muscles of any animal. Histology was performed on 2 of the steers. The main microscopic features were necrotizing myocarditis with myriad intralesional gram-positive rods with subterminal spores plus fibrinosuppurative pericarditis and pleuritis. C. chauvoei was detected by immunohistochemistry and PCR in the myocardium of both animals. These findings confirm a diagnosis of cardiac blackleg in these 2 steers and presumptively in the other affected animals.

show abstract

Section: Diagnosismentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

See 1 more Smart Citation

A review of cardiac blackleg in cattle, and report of 2 cases without skeletal muscle involvement in Argentina

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Initial suspicion of blackleg as the cause of death in an animal is often the result of the history and postmortem lesions, along with clinical signs if observed. 1 A history of rapid onset of signs or death in young, well-nourished cattle indicates that blackleg should be included on the differential diagnosis list. 6 Often, characteristic lesions described above are present and highly suggestive of blackleg.…”

Section: Diagnosismentioning

confidence: 99%

Blackleg (Clostridium chauvoei infection) in beef calves

Daly

Miskimins

Good

et al. 2009

The Bovine Practitioner

View full text Add to dashboard Cite

Blackleg (Clostridium chauvoei infection) has long been recognized as a cause of death in calves grazing summer pastures. Clinical signs are not often observed in affected calves due to the peracute nature of the disease, but may include fever, lameness, and swelling and crepitation over large muscle groups, followed by collapse and death. Typical gross and histopathologic lesions in these muscle groups are well-described and include gelatinous, gassy subcutaneous changes, along with well-defined muscle necrosis. Areas of necrosis can also be present in myocardium, diaphragm, and tongue, among other sites. Primary gross lesions consisted of pleuritis and pericarditis in animals examined in this report. More typical blackleg lesions were found in some but not all calves, including necrosis in thigh and abdominal muscles, dark discoloration of diaphragm muscle, and histologic evidence of necrotic myocarditis. Practitioners finding these lesions in animals found dead on pasture should consider C. chauvoei infection as the diagnosis regardless of whether muscle necrosis lesions are present.

show abstract

Blackleg

Abreu¹,

Uzal²

2016

Clostridial Diseases of Animals

View full text Add to dashboard Cite

Detection of several clostridia by a direct fluorescent antibody test in formalin-fixed, paraffin-embedded tissues

Cited by 4 publications

References 5 publications

A review of cardiac blackleg in cattle, and report of 2 cases without skeletal muscle involvement in Argentina

A review of cardiac blackleg in cattle, and report of 2 cases without skeletal muscle involvement in Argentina

Blackleg (Clostridium chauvoei infection) in beef calves

Blackleg

Contact Info

Product

Resources

About