Detection of sequences from a potentially novel strain of cell fusing agent virus in Mexican Stegomyia (Aedes) aegypti mosquitoes

Espinoza‐Gómez, Francisco; López-Lemus, Alejandro U.; Rodríguez‐Sánchez, Iram P.; Martínez‐Fierro, Margarita L.; Newton-Sánchez, Óscar Alberto; Chávez-Flores, Edgar; Delgado‐Enciso, Iván

doi:10.1007/s00705-011-0967-2

Cited by 33 publications

(23 citation statements)

References 17 publications

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“…CFAV NS5 and NS3 proteins had the greatest level of amino acid identity with other flaviviruses at 45% and 34%, respectively. Since these initial isolation and characterization studies, the presence of CFAV has been detected in feral mosquito pools in multiple regions in both Asia (Hoshino et al, 2009;Yamanaka et al, 2013) and the Americas (Cook, 2006;Espinoza-Gómez et al, 2011). In addition to field-captured mosquitoes evidence of CFAV infection in colonized laboratory mosquitoes has been demonstrated (Bolling et al, 2015a).…”

Section: Cell Fusing Agent Virusmentioning

confidence: 97%

Insect-Specific Viruses

Roundy

Azar

Rossi

et al. 2017

Advances in Virus Research

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Section: Cell Fusing Agent Virusmentioning

confidence: 97%

Insect-Specific Viruses

Roundy

Azar

Rossi

et al. 2017

Advances in Virus Research

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“…Since then, it has been detected in wild-caught mosquitoes in Puerto Rico (Cook et al, 2006), Thailand (Kihara et al, 2007 and Mexico (Espinoza-Gó mez et al, 2011). Over the past 40 years, many other ISFs have been isolated and identified from field-collected mosquitoes, belonging to different species, in several locations (Ferreira et al, 2013;Haddow et al, 2013; Papa et al, 2014 Calzolari et al, 2010a Calzolari et al, , b, 2012aCalzolari et al, , 2013aCerutti et al, 2012;Ravanini et al, 2012;Pautasso et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Wide detection of Aedes flavivirus in north-eastern Italy – a European hotspot of emerging mosquito-borne diseases

Grisenti

Vázquez

Herrero

et al. 2015

Journal of General Virology

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The pattern of flavivirus infection in mosquitoes belonging to the genera Aedes and Culex collected in two regions of north-eastern Italy (Trentino and Veneto) was assessed. Mosquitoes were collected during 2012 and screened for flaviviruses using a generic reverse transcriptionnested-PCR targeted on a region of the non-structural NS5 gene. The phylogenetic analysis was performed on a fragment of~1000 bp. Virus isolation was attempted in C6/36 insect cell lines and the infected cell cultures were studied by electron microscopy. We detected a wide distribution of Aedes flavivirus (AeFV) in Aedes albopictus, with higher infection prevalence in Trentino than in Veneto. In Culex pipiens collected in Veneto, we detected a new sequence of an insect-specific flavivirus and one of Usutu virus. Interestingly, we detected AeFV in C. pipiens, for the first time to our knowledge, in both regions. Viral isolation in cell culture was successful for AeFV. AeFV sequences found in Veneto showed a high percentage of similarity to those detected in Trentino and to those previously reported in other areas of northern Italy. Co-infections with different flaviviruses were not detected. INTRODUCTIONThe genus Flavivirus (family Flaviviridae) comprises .70 viruses that, according to their mechanism of transmission, are included in one of the following three groups: (1) those infecting a range of vertebrate hosts through mosquito or tick bites, called 'arthropod-borne viruses', (2) those spread without a known vector, presumed to be limited to infecting vertebrates only, and (3) those apparently limited to insects alone, called 'insect-specific flaviviruses' (ISFs) (Ishikawa & Konishi, 2011;Huhtamo et al., 2012 and references therein). The flavivirus genome contains genes coding for three structural proteins (capsid, premembrane and envelope) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) (Lindenbach et al., 2006). Regions encoding envelope, NS3 and NS5 are the most frequently used for phylogenetic analysis of flaviviruses. The inclusion of ISFs in the genus Flavivirus is supported by similarities with other flaviviruses in terms of genomic organization, polyprotein hydropathy profiles and cleavage sites, but they are not able to replicate in mammalian cells and they have been isolated only in mosquito-derived cells (Kuno, 2007;Hoshino et al., 2009;Bolling et al., 2011;Cook et al., 2012;Haddow et al., 2013).The 'arthropod-borne viruses' group includes some important emerging human and animal pathogens, such 3These authors contributed equally to this work.The GenBank/EMBL/DDBJ accession numbers for representative sequences obtained in this work are KM871198-KM871202. Lelli et al., 2008;Manarolla et al., 2010;Tamba et al., 2011; Vázquez et al., 2011;Cerutti et al., 2012;Ravanini et al., 2012;Buchebner et al., 2013;Calzolari et al., 2013a; Höfle et al., 2013). In Europe, USUV has been recognized as a candidate human pathogen in Austria (Weissenböck et al., 2007), Italy (Cavrini et al., 2009;Pecorari et al., ...

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“…Subsequently, several strains of the virus have also been detected in field-collected A. aegypti and other mosquito species Espinoza-Gómez et al, 2011;Hoshino et al, 2007;Kihara et al, 2007;Sang et al, 2003;Yamanaka et al, 2013). CFAV has extensive similarities to medically important flaviviruses in terms of genome size, structure and gene order (Cammisa-Parks et al, 1992).…”

mentioning

confidence: 99%

Wolbachia suppresses cell fusing agent virus in mosquito cells

Zhang

Etebari

Asgari

2016

Journal of General Virology

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The genus Flavivirus contains a large number of positive-sense ssRNA viruses. While some are transmitted by mosquitoes or other arthropods and are pathogenic to humans and animals (e.g. dengue and Zika viruses), some are insect-specific and do not replicate in vertebrate cells. These are known as insect-specific flaviviruses (ISFs). Cell fusing agent virus (CFAV) was the first described ISF, which was detected in an Aedes aegypti cell line, Aag2. Here, we investigated the effect of Wolbachia, a widespread endosymbiont of many insect species, that is known to block replication of several pathogenic flaviviruses, on CFAV. Our results demonstrated that, in mosquito cells, Wolbachia vastly suppresses replication of CFAV, with significantly less CFAV viral interfering small RNAs produced in the cells. However, removal of Wolbachia with tetracycline led to increased CFAV replication. These results suggest that Wolbachia is also able to suppress an ISF.

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Detection of sequences from a potentially novel strain of cell fusing agent virus in Mexican Stegomyia (Aedes) aegypti mosquitoes

Cited by 33 publications

References 17 publications

Insect-Specific Viruses

Insect-Specific Viruses

Wide detection of Aedes flavivirus in north-eastern Italy – a European hotspot of emerging mosquito-borne diseases

Wolbachia suppresses cell fusing agent virus in mosquito cells

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