Detection of Selenocysteine with a Ratiometric near-Infrared Fluorescent Probe in Cells and in Mice Thyroid Diseases Model

Abstract

The pathological progression of thyroid diseases poses a serious threat to human health. Because thyroid diseases are closely related to selenocysteine (Sec), it is necessary to investigate the relationship between Sec and thyroid diseases. Herein, we design and synthesize a ratiometric near-infrared fluorescent probe (Mito-Cy-Sec) to analyze the fluctuations and roles of Sec in cells and in mice thyroid diseases model. The probe is composed of a nearinfrared heptamethine cyanine fluorophore, an acrylamide as the response moiety, and a lipophilic triphenylphosphonium cation as the mitochondrial localization group. After reacting with Sec for 5 min, the probe Mito-Cy-Sec exhibits a distinct ratiometric fluorescence signal accompanied by a color change from green to blue. The applicability of Mito-Cy-Sec in mitochondrial localization is assessed via the super-resolution imaging. Mito-Cy-Sec has been successfully applied to detect the fluctuations of Sec concentration in human thyroid epithelial/cancer cell lines (Nthy-ori-3 cells/BHT101 cells) and mice thyroid disease (thyroiditis and thyroid cancer) models. Besides, both of our probes Mito-Cy-Sec and commercial ROSGreen H 2 O 2 are employed to examine the interrelationship between H 2 O 2 and Sec in cells and in mice models. The results demonstrate that the relevant-levels between H 2 O 2 and Sec are exactly negative correlation. The related-levels of Sec and H 2 O 2 may be identified as diagnostic indicators for the auxiliary diagnosis of thyroid diseases. We suppose that our probe Mito-Cy-Sec can be employed as a promising chemical tool for the diagnosis of thyroid diseases.

Design and synthesis of a ratiometric photoacoustic imaging probe activated by selenol for visual monitoring of pathological progression of autoimmune hepatitis

“…This is attributed to the involvement of selenol in the anti-inflammatory response which leads to its consumption. 31 These findings indicate that APSel has the potential to be used for ratiometric PA imaging of the changes of endogenous selenol levels.…”

Regulating Twisted Skeleton to Construct Organ‐Specific Perylene for Intensive Cancer Chemotherapy

“…Considering that high intracellular H 2 O 2 in the cancer cells, [49–52] it is purposed that the enhanced ROS in A549 and H446 is probably H 2 O 2 . To verify the suppose, we firstly evaluate the production of H 2 O 2 by commercially available fluorescent probe ROSGreenTM [53] . It can be seen in Figure S21 that A549 and H446 cells incubated with PDIC‐NC (1 μg mL −1 ) for 6 h exhibited 10‐fold higher green fluorescence than PBS‐treated cells.…”

A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

“…At the same time, the concentration of Sec in normal cells is very low. However, when there are some physiological abnormalities, such as thyroid disease, the concentration of Sec will increase significantly, and the fluorescence sensors can detect the Sec concentration [ 23 , 26 ]. Therefore, YZ-A4 can be used to detect Sec concentration in abnormal cells of simulated physiological diseases.…”

“…Though a few long-wavelength Sec fluorescent sensors have been reported in recent years [ 24 , 25 , 26 ], further development research is still needed to date, such as developing fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength Sec fluorescent sensors. Hence, we rationally designed a novel turn-on sensor YZ-A4 based on xanthene dyes, and using a 2,4-dinitrobenzene sulfonate ester as the reaction sites of Sec.…”