Detection of Salmonella Spp. in Feed and Their Antibiotic Susceptibility for Alternative Therapy

Vu, Truong Huynh Anh; Huu, Nguyen Ngoc; Ly, Ha Dieu; Tu, Nguyen Hoang Khue

doi:10.7324/japs.2016.60503

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…The protocol was followed as the previous study. 21 The same strains that showed many serovars with the sulfonamide resistance were used to the detect sul1 and sul2 by PCR to clarify or find out more whether sul1, sul2 or both could affect the resistance as many previous studies concluded before.…”

Section: Salmonella Spp Isolation and Identificationmentioning

confidence: 99%

Antibiotic Resistance in Salmonella Isolated from Ho Chi Minh City (Vietnam) and Difference of Sulfonamide Resistance Gene Existence in Serovars

Vu¹,

Hai²,

Ha³

et al. 2021

J. Pure Appl. Microbiol.

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In this study, a total of 228 raw meat samples (pork: 76, beef: 76, chicken: 76) and 301 raw seafood samples (fish: 199, shrimp: 67, squid: 35) were collected randomly at traditional markets in Ho Chi Minh City (Vietnam). In meat, the ratio of Salmonella spp. was 70.61% (161/228). Among the contaminated meat samples, pork was infected with a ratio of 90.79 % (69/76) while the contamination ratios in beef and chicken were 43.42% (33/76) and 77.63% (59/76), respectively. Salmonella contamination was detected in fish (40.20%), shrimp (7.46%) and squid (17.14%). Because of sulfonamide group is used in Salmonella treatment, the study focused on sulfonamide resistance. In fresh seafood comparison, there were 32.56%, 40% and 10% Salmonella showing resistance to sulfamethoxazol in fish, squid and shrimp, respectively. In fresh meat comparison, there were 31.58%, 16.67% and 55.56% Salmonella showing resistance to sulfamethoxazol in pork, beef and chicken, respectively. Interestingly, there were 21 serovars including 19 identified serovars including S. Kentucky (8), S. Agona (2), S. Infanis (4), S. Saintpaul (1), S. Indiana (1), S. Braenderup (1), S. Potsman (2) and 2 unidentified serovars showing different phenotype to this antibiotic. Among the 21 serovars, only 23.81% strains carried both genes (sul1, sul2). For the sul1 gene, 61.9% strains were presented while sul2 occupied at a lower rate than sul1 with the rate of 52.38%. The study is very interesting and useful to go more functional analysis in sulfonamide resistance.

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Section: Salmonella Spp Isolation and Identificationmentioning

confidence: 99%

Antibiotic Resistance in Salmonella Isolated from Ho Chi Minh City (Vietnam) and Difference of Sulfonamide Resistance Gene Existence in Serovars

Vu¹,

Hai²,

Ha³

et al. 2021

J. Pure Appl. Microbiol.

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“…Salmonella persists in a wide range of feedstuffs for a long period (Abd El-Ghany et al 2015). Therefore, detection and elimination of Salmonella in feed are necessary in the processing chain guarantees (Vu et al 2016). Since all feed ingredients can be potential Salmonella source, decontamination steps are essential to prevent spreading of contaminated feed to production animals (Sauli et al 2005;Hald et al 2012;Vukmirović et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of Nisin and Organic Acid on Salmonella enteritidis Colonization in Poultry Feeds at Different Storage Time

Üstündağ

2021

Hayvansal Üretim

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Objective: This study was conducted to investigate the inhibitory effects of nisin alone or in combination with organic acid on Salmonella enteritidis in poultry feed at different storage times.Material and Methods: Feeds divided into six experimental groups that consist of control, 150 mg/kg nisin (N150), 300 mg/kg nisin (N300), 3 g/kg organic acid mixture (OA), 150 mg/kg nisin + 3 g/kg organic acid mixture (N150+OA), 300 mg/kg nisin + 3 g/kg organic acid mixture (N300+OA). Then, 10 4 cfu/ml Salmonella enteritidis cultures added in the feeds. Feeds were stored in room temperature and Salmonella colonies were enumerated at 48 th hour (Initial Time), followed on 7 th , 15 th , 21 st and 28 th days of the study. Results:The highest Salmonella values were observed in the control group during this study (P<0.05). Nisin that alone and in combination with organic acid had inhibitory effects against Salmonella enteritidis at initial time, on 7 th , 15 th , 21 st and 28 th days of the study. However, on the 28 st day, an increase of Salmonella count in the experimental groups was observed. Conclusion:It is known that the effect of nisin on gram-negative bacteria is low. However, in this study, it was observed that a significant decrease in the number of Salmonella occurred with the addition of nisin to the feeds stored at room temperature for 28 days. ÖZ Amaç: Bu çalışma, farklı depolama sürelerinde kanatlı hayvan yemlerinde tek başına veya organik asit ile kombinasyon halinde nisinin Salmonella enteritidis üzerine önleyici etkilerini araştırmak için yapılmıştır.Materyal ve Metot: Yemler; kontrol, 150 mg/kg nisin (N150), 300 mg/kg nisin (N300), 3 g/kg organik asit karışımı (OA), 150 mg/kg nisin + 3 g/kg organik asit karışımı (N150+OA), 300 mg/kg nisin + 3 g/kg organik asit karışımı (N300+OA) dan oluşan 6 deneme grubuna ayrılmıştır. Daha sonra yemlere 10 4 cfu/ml Salmonella enteritidis kültürü eklenmiştir. Yemler oda sıcaklığında muhafaza edilmiş ve denemenin 48. saatinde (başlangıç Zamanı), 7, 15, 21 ve 28. günlerinde Salmonella sayımı yapılmıştır. Bulgular: Bu çalışmada en yüksek Salmonella değerleri Kontrol grubunda gözlenmiştir (P<0.05). Nisin, tek başına ve organik asit ile kombinasyon halinde, çalışmanın başlangıç zamanında, 7, 15, 21 ve 28. günlerinde Salmonella enteritidis'e karşı inhibe edici etkilere sahip olduğu gözlenmiştir. Bununla birlikte, 28. günde, deneme gruplarında Salmonella sayısında bir artış gözlenmiştir. Sonuç: Nisinin gram negatif bakteriler üzerindeki etkisinin düşük olduğu bilinmektedir. Bununla birlikte, bu çalışmada 28 gün oda sıcaklığında saklanan yemlere nisin ilavesiyle Salmonella sayısında önemli bir azalma olduğu gözlenmiştir.

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Detection of Salmonella Spp. in Feed and Their Antibiotic Susceptibility for Alternative Therapy

Cited by 2 publications

References 2 publications

Antibiotic Resistance in Salmonella Isolated from Ho Chi Minh City (Vietnam) and Difference of Sulfonamide Resistance Gene Existence in Serovars

Antibiotic Resistance in Salmonella Isolated from Ho Chi Minh City (Vietnam) and Difference of Sulfonamide Resistance Gene Existence in Serovars

Effects of Nisin and Organic Acid on Salmonella enteritidis Colonization in Poultry Feeds at Different Storage Time

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