Detection of Rubella-specific IgM in Subclinical Rubella Reinfection in Pregnancy

Morgan‐Capner, P.; Hambling, M.H.; Coleman, T. J.; Watkins, R; Stern, H.; Hodgson, Julian; Dulake, C.; Boswell, P.A; Booth, J. C.; Best, Jennifer M.; Banatvala, J. E.

doi:10.1097/00006254-198509000-00006

Cited by 17 publications

(21 citation statements)

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“…As specific IgM can be detected both in primary rubella and rubella reinfection, the serological differentiation can no longer be based solely on its detection (Morgan-Capner et al 1985). We have previously investigated the rubella-specific IgG subclass profile in primary rubella and rubella reinfection (Thomas & MorganCapner, 1988).…”

Section: Introductionmentioning

confidence: 99%

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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SUMMARYAn antiglobulin enzyme-linked immunosorbent assay for rubella-specific IgG1 and IgG3 was adapted to measure antibody avidity by incorporating a mild protein denaturant, diethylamine (DEA), into the serum diluent. Sera were tested at varying dilutions, both with and without DEA, if they contained sufficient specific IgG, or IgG3. The optical density (OD) was measured and curves were plotted. The highest OD (V) was noted and halved (V/2). The distance between the OD curves at V/2 was measured as the DEA shift value.Sera were examined from people whose sera contained rubella-specific antibodies as a consequence of infection or vaccination in the distant past (24 sera), recent primary rubella (66 sera), symptomatic reinfection (11 sera) or asymptomatic reinfection (64 sera). For specific IgG1 the DEA shift value was < 0W6 for cases of rubella in the distant past, compared with > 0-8 for the first month after primary infection. The maximum DEA shift value for the sera from cases of reinfection was 0-65.No serum from cases of rubella in the distant past contained sufficient specific IgG3 to estimate avidity. The sera collected within 1 month of onset of primary rubella gave DEA shift values > 0 7 compared with sera from reinfections, which gave DEA shift values < 0 6, except for two sera from a case of symptomatic reinfection.Thus the assessment of specific IgG subclass avidity is of value in differentiating serologically primary rubella from reinfection.

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Section: Introductionmentioning

confidence: 99%

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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“…The majority had no clinical illness, but three cases had had a rubelliform illness with detectable specific IgM and two have been previously reported (MorganCapner et al 1983;. Many of the asymptomatic reinfections examined have also been reported previously (Cradock-Watson et al 1981; Morgan-Capner et al 1985). In five sera from four cases of asymptomatic reinfection rubella-specific IgM had been detected by MACRIA (all < 10 au).…”

Section: Seramentioning

confidence: 55%

“…If previous results or early sera are not available, primary rubella may be diagnosed by detecting substantial concentrations of specific IgM, whereas in reinfection specific IgM is usually undetectable or present in only low concentration. However, the recent demonstration of higher concentrations of specific IgM in reinfection means that occasionally it may be impossible to distinguish reinfection from asymptomatic primary infection using currently available tests for rubella-specific IgG and IgM (Morgan-Capner et al 1985).…”

Section: Introductionmentioning

confidence: 99%

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Specific IgG subclass antibody in rubella virus infections

Thomas

Morgan‐Capner

1988

Epidemiol. Infect.

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SUMMARYA solid-phase antigen enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rubella-specific IgG subclasses. For rubella-specific IgG, and IgG3 sera were quantitated in arbitrary units (au) by comparison with standard curves. A concentration of 3 au was taken as that indicating positivity for specific IgG1 and specific IgG3. No sera reactive for specific IgG2 and IgG4 have been found, and thus the assay reagents were controlled by testing dilutions of a standard calibrant serum containing known concentrations of the specific IgG subclasses.Of 105 unselected sera negative for rubella antibody by radial haemolysis (RH), two gave concentrations of specific IgG, > 3 au and both were positive by rubella latex agglutination (LA).

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“…Comparison with the antibody responses seen in immunocompetent individuals (2,3,15) suggested that the transient low concentration of specific IgM with a high concentration of specific IgG, and a low concentration of specific IgG3 reflected a reinfection.…”

Section: Discussionmentioning

confidence: 99%

Laboratory diagnosis and clinical significance of rubella in children with cancer

et al. 1989

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SUMMARYVirus-specific antibody responses were studied in 12 children with cancer in whom rubella was diagnosed by seroconversion or a rising titre (>s fourfold) of haemagglutination inhibiting (HI) antibody. Our results confirmed the difficulties of making a diagnosis of rubella infection in immunocompromised children using criteria for interpreting antibody assays established in immunocompetent patients. Specific IgM antibody persisted for more than 2 months in 7 of 10 children with probable primary rubella, 3 of whom had high concentrations of such antibody 6, 7 and 11 months after the rash. Radial haemolysis and specific IgG, and IgG3 antibody responses were low in 4, 2, and 4 patients, respectively.

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Detection of Rubella-specific IgM in Subclinical Rubella Reinfection in Pregnancy

Cited by 17 publications

References 0 publications

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Rubella-specific IgG subclass avidity ELISA and its role in the differentiation between primary rubella and rubella reinfection

Specific IgG subclass antibody in rubella virus infections

Laboratory diagnosis and clinical significance of rubella in children with cancer

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