Detection of Rift Valley fever virus in mosquitoes by RT-PCR

Ibrahim, M. Sofi; Turell, Michael J.; Knauert, F. K.; Lofts, Richard S.

doi:10.1006/mcpr.1996.0075

Cited by 45 publications

(25 citation statements)

References 9 publications

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“…ELISAs and inoculation into hamsters are both less susceptible to inhibition than plaque assay but are also less sensitive. PCR is both rapid and sensitive but extraction methods should be evaluated to limit the presence of inhibitory substances in RNA extracts from mosquito samples [96,97]. These methods can be streamlined to a high-throughput format to allow the processing of large numbers of individual mosquitoes.…”

Section: Detection Methodsmentioning

confidence: 99%

Rift Valley fever virus: strategies for maintenance, survival and vertical transmission in mosquitoes

Lumley

Horton

Hernandez-Triana

et al. 2017

Journal of General Virology

101

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Rift Valley fever virus (RVFV) is a mosquito-borne arbovirus causing severe disease in humans and ruminants. Spread of RVFV out of Africa has raised concerns that it could emerge in Europe or the USA. Virus persistence is dependent on successful infection of, replication in, and transmission to susceptible vertebrate and invertebrate hosts, modulated by virushost and vector-virus interactions. The principal accepted theory for the long-term maintenance of RVFV involves vertical transmission (VT) of virus to mosquito progeny, with the virus surviving long inter-epizootic periods within the egg. This VT hypothesis, however, is yet to be comprehensively proven. Here, evidence for and against the VT of RVFV is reviewed along with the identification of factors limiting its detection in natural and experimental data. The observations of VT for other arboviruses in the genera Alphavirus, Flavivirus and Orthobunyavirus are discussed within the context of RVFV. The review concludes that VT of RVFV is likely but that current data are insufficient to irrefutably prove this hypothesis.

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Section: Detection Methodsmentioning

confidence: 99%

Rift Valley fever virus: strategies for maintenance, survival and vertical transmission in mosquitoes

Lumley

Horton

Hernandez-Triana

et al. 2017

Journal of General Virology

101

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“…Considerable efforts have been made recently to develop nucleic acid techniques for rapid detection and identification of RVFV (Ibrahim et al, 1997 andGarcia et al, 2001). …”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of Rift Valley fever virus nucleocapsid (N) protein and evaluation of a N-protein based indirect ELISA for the detection of specific IgG and IgM antibodies in domestic ruminants

Fafetine¹,

Tijhaar²,

Pawęska³

et al. 2007

Veterinary Microbiology

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“…PCR has been successfully applied in the diagnosis of VHF (7,8,22,28,30,31,36). However, most of the published assays are time-consuming, as they include a separate cDNA synthesis step prior to PCR, agarose gel analysis of PCR products, and in some instances a second round of nested amplification or Southern hybridization.…”

mentioning

confidence: 99%

Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

et al. 2002

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Viral hemorrhagic fevers (VHFs) are acute infections with high case fatality rates. Important VHF agents are Ebola and Marburg viruses (MBGV/EBOV), Lassa virus (LASV), Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), dengue virus (DENV), and yellow fever virus (YFV). VHFs are clinically difficult to diagnose and to distinguish; a rapid and reliable laboratory diagnosis is required in suspected cases. We have established six one-step, real-time reverse transcription-PCR assays for these pathogens based on the Superscript reverse transcriptase-Platinum Taq polymerase enzyme mixture. Novel primers and/or 5-nuclease detection probes were designed for RVFV, DENV, YFV, and CCHFV by using the latest DNA database entries. PCR products were detected in real time on a LightCycler instrument by using 5-nuclease technology (RVFV, DENV, and YFV) or SybrGreen dye intercalation (MBGV/EBOV, LASV, and CCHFV). The inhibitory effect of SybrGreen on reverse transcription was overcome by initial immobilization of the dye in the reaction capillaries. Universal cycling conditions for SybrGreen and 5-nuclease probe detection were established. Thus, up to three assays could be performed in parallel, facilitating rapid testing for several pathogens. All assays were thoroughly optimized and validated in terms of analytical sensitivity by using in vitro-transcribed RNA. The >95% detection limits as determined by probit regression analysis ranged from 1,545 to 2,835 viral genome equivalents/ml of serum (8.6 to 16 RNA copies per assay). The suitability of the assays was exemplified by detection and quantification of viral RNA in serum samples of VHF patients.

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Detection of Rift Valley fever virus in mosquitoes by RT-PCR

Cited by 45 publications

References 9 publications

Rift Valley fever virus: strategies for maintenance, survival and vertical transmission in mosquitoes

Rift Valley fever virus: strategies for maintenance, survival and vertical transmission in mosquitoes

Cloning and expression of Rift Valley fever virus nucleocapsid (N) protein and evaluation of a N-protein based indirect ELISA for the detection of specific IgG and IgM antibodies in domestic ruminants

Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

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