Detection of Poultry and Pork in Cooked and Canned Meat Foods by Enzyme-Linked Immunosorbent Assays

Abstract

Enzyme-linked immunosorbent assays (ELISA) are described for the detection of poultry and pork in cooked and canned muat foods. These assays are based on species-specific, polyclonal antibodies raised against heat-resistant antigens. The heat-resistant antigens were isolated from raw skeletal muscle tissue of pork and chicken and were found to be immunoreactive even after heating to 120°C for 15 min. The poultry ELISA could detect chicken or turkey at the 126 ppm level, and the pork ELISA could detect pork at the 250 ppm level. Samples of frankfurters, bolognas, pressed meats, canned baby foods, and canned spreads were prepared by simple aqueous extractions

Rapid identification of grouper and wreck fish meals by ELISA: a field study in restaurants

“…The great limitation of these methods is that proteins are denatured during cooking and therefore the target protein epitope may not be present in the condition detectable by the antibodies. This has been solved because of the development of antibodies against thermostable proteins (Berger et al, 1988;Ansfield et al, 2000).…”

Species identification of meat products by ELISA

“…Identification of beef, pork, sheep and poultry were performed using cooked species identification ELISA kits from Tepnel (ref. CO 911Q, 912N, 913L, 922K, 923H, 972Y) according to the manufacturer's instructions (Berger et al, 1988;Diffchamb France Sarl, 2001;Tepnel Biosystems Limited, 2001). Immunodetection of egg white and milk were performed with a direct homemade ELISA (Demeulemester et al, 1991;Demeulemester & Guizard, 1996;Leduc et al, 1999).…”

“…CO 911Q, 912N, 913L, 922K, 923H, 972Y) from Tepnel (Deeside, UK) for identification of animal species in processed meat products. These kits based on the method initially described by Berger et al (1988) were chosen because they are easy to perform, robust and largely used worldwide.…”

Myoglobin Analysis for Determination of Beef, Pork, Horse, Sheep, and Kangaroo Meat in Blended Cooked Products

“…The method is suitable for screening, but the required antisera are not commercially available; they were primarily meant for use in enforcement of the Endangered Species Act (Kenya). Berger et al (1988) described an immuno-assay to detect chicken meat and pork in heat-processed meat products. Polyclonal antisera were produced against thermostable protein fractions.…”