Detection of Porphyromonas gingivalis from Periodontal Pocket Infections by Microbial Cultivation and PCR  Techniques

Al-Rawi, Amera M.

doi:10.33899/rjs.2012.27971

Cited by 2 publications

(2 citation statements)

References 10 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gram positive bacteria were dominant in our study (88%), the consequences revealed that 16 (29.5) of isolate revealed positive bacterial culture of S. viridians (Table 1 & Figure 1) whereas 13 (24), 11 (20) isolates of Streptococcus. pyogenes and Staphylococcus aureus respectively with medium percentage of isolates belong to Lactobacillus spp.…”

Section: Discussionmentioning

confidence: 51%

Systemic Immunological Responses Among Dental Infection Patients

Mahdi¹,

Abood²

2021

IJI

View full text Add to dashboard Cite

Evaluating of some immunological responses among dental infection patients is essential in the clinical management of oral infection. Our study considered isolation of dental infection bacteria and quantitative evaluation of serum. IgA, IL-4, IL-7 and CD4 and CD8 molecules among dental plaque patients and control group. Oral bacteria from dental infection patients were isolated in appropriate media and diagnosed by biochemical tests and in vitro quantitative determination of serum IgA, IL-4, IL-7 and CD4 and CD8 molecules using ELISA technique. Single and mixed bacterial isolates were noted, mixed infection were (59.25%), the nature of bacteria was Gram positive cocci Streptococcus viridans, Streptococcus pyogenes and Staphylococcus aureus, and Lactocacilli spps, in addition to Gram negative rods black-pigmented bacteria, Klebsilla pneumonia, and Esherishia coli. Serum IgA was higher in patients (368.8±182.5) ng\ml than in control group (319.92±79.26) ng\ml. Serum IL-4 was higher patients (285.33±86.12) pg/ml than in control group (257.7±94.14) pg\ml. Serum IL-7 was higher in control group (19.59±4.14) pg/ml than in dental plaque patients (17.98±3.18) pg /ml. Serum CD4 molecules was higher in control group (1.371±0.5242) ng/ml than in dental plaque patients (1.326±0.1292) ng/ml. Serum CD8 molecules shows non-significant elevation in patients group 0.5825±0.02717 (ng\ml) than in control group 0.51±0.01643 (ng\ml) P≤0.05. Mean of the CD4/CD8 ratio was higher 2.783±1.126 in control group while it was 2.355±0.24 in dental plaque patients, however the differences were non-significant (P≤0.05). The present study conclude that the bacteria isolated from dental infection patients were mixed more than single infection, there were non-significant elevation in IgA, IL-4, and CD8 in patients while IL-7 and CD4 was lower in patients group than in control group, while CD4\CD8 ratio were lower in patients group, these result reflect the fact that mucosal antigen induce systemic tolerance to some extent since these bacteria present in oral cavity in early childhood. Therefor removing these bacteria always the best way to prevent such infections.

show abstract

Section: Discussionmentioning

confidence: 51%

Systemic Immunological Responses Among Dental Infection Patients

Mahdi¹,

Abood²

2021

IJI

View full text Add to dashboard Cite

show abstract

“…The bacteriological study including cultural properties, microscopic examination and biochemical tests was done according to MacFaddin et al, [9] ,Forbes et al, [10] . Al-Rawi [11] . "Statistical analysis castoff in this study includes calculation of mean values and percentage".…”

Section: Methodsmentioning

confidence: 99%

Isolation and Identification of Bacterial Species Associated With Odontogenic Infections

Kzar¹,

Abood²,

Abd³

et al. 2020

ATMPH

View full text Add to dashboard Cite

Odontogenic infection is an infection that originates within a teeth and the closely surrounding tissues as the dental pulp, jawbones, periodontium, or in closely surrounding tissue. This study aimed to isolate and detect bacteria associated with odontogenic infections and identify antimicrobial susceptibility against predominant bacterial isolates.Atotal of 30 pus samples were collected by aspiration and placed in transport media. "These pus samples subjected to different methods for identification of bacteria using traditional bacteriological method". The antimicrobial liability of isolated bacteria was determined by using disc diffusion method.Predominant isolated bacteria was Streptococcusviridians group (36%) followed by S. aureus (26.2%) and lowest percentage of isolated bacteria was black pigmented bacteria(Prevotella and Porphyromonasspp), in addition to K. Pneumonia and E. coli. The results showed very high susceptibility of isolates to amoxicillin (97%) followed by cefotaxime and imipenem (94%). Whereas the lowest sensitivity was detected to clindamycin (18%).The present results approve the existence of mixed bacterial infection with the predominance of Streptococcusviridans. Most Streptococcusviridans were inhibited by amoxicillin followed by cefotaxime and imipenem.

show abstract

Detection of Porphyromonas gingivalis from Periodontal Pocket Infections by Microbial Cultivation and PCR Techniques

Cited by 2 publications

References 10 publications

Systemic Immunological Responses Among Dental Infection Patients

Systemic Immunological Responses Among Dental Infection Patients

Isolation and Identification of Bacterial Species Associated With Odontogenic Infections

Contact Info

Product

Resources

About