Detection of Polymorphism in Exon 2 of Toll-like Receptor 4 Gene of Indian Buffaloes Using PCR-SSCP Technique

Dubey, Praveen; Selvakumar, M.; Kathiravan, P.; Yadav, Neelam; Mishra, Bina; Kataria, R. S.

doi:10.1080/09712119.2010.9707138

Cited by 2 publications

(2 citation statements)

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“…River buffaloes were found to be differentially susceptible/ resistant to different bacterial (Mastitis and Haemorrhagic septicaemia), viral (infectious bovine rhino-tracheitis) and protozoan (Theileria, Babesia) diseases when compared to ruminants especially with closely related cattle (Bain et al, 1982;Dua, 2001;Ravindran et al, 2007). The only report on bubline TLR available to date is on the structure of TLR 3 (Dhara et al, 2007) and single-strand conformation polymorphism of partial TLR 4 gene (Dubey et al, 2010). Hence, the present study was undertaken to characterize all the ten TLR genes of river buffalo and to assess their genetic and evolutionary relationship with TLRs of other domestic animal species using conceptualized amino acid sequence information.…”

Section: Introductionmentioning

confidence: 99%

Sequence characterization of river buffalo Toll‐like receptor genes 1–10 reveals distinct relationship with cattle and sheep

Dubey

Goyal

Kathiravan

et al. 2012

Int J Immunogenetics

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The present study was undertaken to characterize the full-length transcripts of Toll-like receptor (TLR) genes 1-10 of river buffalo. The conceptualized amino acid identity of bubaline TLRs ranged between 86% to 100% with ruminants, while it ranged between 45% to 91% with other vertebrate species. Simple modular architecture tool (SMART) analysis revealed the presence of TIR domains and varying numbers of leucine-rich repeat motifs in all the buffalo TLRs. With respect to TIR domains, TLRs 1, 2 and 3 of river buffalo were found to have 99.3% identity with cattle and 100% identity of TLRs 4, 6 and 10 with sheep. Phylogenetic analysis of TLRs of buffalo and different vertebrate species revealed the clustering of major TLR gene subfamilies with high bootstrap values. The evolutionary relationship between buffalo and other ruminant species was found to vary among different TLRs. In order to understand the relationship between TLRs of different ruminant species, multidimensional scaling (MDS) analysis of pairwise amino acid differences between different species within each TLR was performed. Buffalo and cattle were found to be closely related only with respect to TLRs 1, 2 and 7, while buffalo and sheep were found to be clustering together with respect to TLRs 3, 6, 8 and 10. The distinct relationship of bubaline TLRs with cattle and sheep revealed the possible differences in the pathogen recognition receptor systems in these animals and consequently the differences in their susceptibility/resistance to various invading organisms.

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Section: Introductionmentioning

confidence: 99%

Sequence characterization of river buffalo Toll‐like receptor genes 1–10 reveals distinct relationship with cattle and sheep

Dubey

Goyal

Kathiravan

et al. 2012

Int J Immunogenetics

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“…Since it is not possible to utilize the costlier techniques like sequencing for the genotyping of SNPs, the simple and cheaper methods of SSCP, PCR-RFLP, etc. have been utilized by many workers to find the allelic variation in TLR genes for different purposes (Zhang et al 2009; Baris et al 2010;Dubey et al 2010). Since many workers have found correlation of polymorphism in TLR8 gene with the susceptibility to various diseases in different species (Davila et al 2008;Mikula et al 2010), this variation needs to be confirmed for such association if any, which further needs the generation of phenotypic data on disease resistance and susceptibility.…”

Section: Resultsmentioning

confidence: 99%

Development of tetra-primers ARMS-PCR assay for the detection of A1551G polymorphism in TLR8 gene of riverine buffalo

Dubey

Goyal

Aggarwal

et al. 2012

Journal of Applied Animal Research

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In this study, a simple PCR-based tetra-primers amplification refractory mutation system (ARMS)-PCR technique has been developed to screen one of the synonymous Single Nucleotide Polymorphism (SNPs) 1551A ! G found in buffalo TLR8 gene. The technique was employed to screen 152 genomic DNA samples represented by various riverine and swamp buffaloes. Analysis of data revealed presence of all the three AA, A/G and GG genotypes in Chilika buffalo Á whereas only genotype GG was found in all other riverine and swamp buffaloes. The results indicate allele 'A' to be Chilika specific and that the technique of tetra-primers ARMS-PCR can be used as a cost-effective, alternate method to differentiate the genotypes.

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Detection of Polymorphism in Exon 2 of Toll-like Receptor 4 Gene of Indian Buffaloes Using PCR-SSCP Technique

Abstract: Dubey, P.K., Selvakumar, M., Kathiravan, P., Yadav, N., Mishra, B.P. and Katana, R.S. 2010

Cited by 2 publications

References 9 publications

Sequence characterization of river buffalo Toll‐like receptor genes 1–10 reveals distinct relationship with cattle and sheep

Sequence characterization of river buffalo Toll‐like receptor genes 1–10 reveals distinct relationship with cattle and sheep

Development of tetra-primers ARMS-PCR assay for the detection of A1551G polymorphism in TLR8 gene of riverine buffalo

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