Sequence characterization of river buffalo Toll‐like receptor genes 1–10 reveals distinct relationship with cattle and sheep

Dubey, Praveen; Goyal, Shweta; Kathiravan, P.; Mishra, Bina; Gahlawat, Suresh Kumar; Kataria, R. S.

doi:10.1111/j.1744-313x.2012.01135.x

Cited by 14 publications

(20 citation statements)

References 26 publications

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“…Specifically, the mRNA levels of TLR2 and TLR4 increase in endometritis infection of bacterial origin (Ozinsky et al, 2000;Goldammer et al, 2004;Azawi et al, 2008;Davies et al, 2008). With the agreement of these data, we detected overexpression of all five examined TLR genes, particularly TLR2 (26.4 fold) and TLR4 (12.4 fold), in infected uterine tissues with endometritis compared to the uninfected ones.…”

Section: Discussionsupporting

confidence: 84%

“…The number and structure of LRRs of TLRs have a significant effect on the function of pathogen recognition receptor system among species (Kumar et al, 2009;Dubey et al, 2013). Peter et al (2009) reported that deletion of LRR motif leads to loss of functional integrity of the ligand-binding domain of murine TLRs.…”

Section: Discussionmentioning

confidence: 99%

“…Peter et al (2009) reported that deletion of LRR motif leads to loss of functional integrity of the ligand-binding domain of murine TLRs. In addition, (Dubey et al, 2013) demonstrated that the differences in the number of LRRs among buffalo and cattle may lead to possible differences in the specific recognition of TLR ligands between these species. In our study we found that the number of LRR domains of Bubalus bubalis with endometritis and their distribution is similar to Bubalus bubalis without endometritis except TLR1 and TLR4.…”

Section: Discussionmentioning

confidence: 99%

“…A TIR domain is required for initiating intracellular signaling and inducing downstream effector molecules, while LRRs are responsible for the recognition of PAMPs. (Akira et al, 2006;Kumar et al, 2009;Dubey et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Transcriptional Profile of Toll-Like Receptors of Bubalus bubalis with Endometritis

Osman¹

2015

J. Inf. Mol. Biol.

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| Toll-like receptors (TLRs) are a vital component of innate immune system and perform an important role in disease resistance through recognition of pathogen associated molecular patterns (PAMPs) and initiation of a convenient immune response. Endometritis is the most important disease causing infertility in cattle and buffalo and TLRs play a critical role in elimination of that disease. The present study aims to characterize the transcripts of TLRs: TLR1, TLR2, TLR4, TLR6 and TLR10 in Bubalus bubalis with endometritis. Quantitative real-time (q-PCR) assays were performed to detect transcript expression profiles of these TLRs in liver, mammary gland, ovary and uterus of Bubalus bubalis with and without endometritis. The result showed that the transcript profiles of TLRs especially TLR2 and TLR4 were significantly differences in mammary glands, ovary and uterus but on the othe hands, there is no significant difference in the transcript profile of TLRs in liver with or without endometritis. TLRs nucleotide sequences have more than 97% nucleotide homology between Bubalus bubalis with and without endometritis. Simple modular architecture tool (SMART) analysis revealed the presence of TIR domains and different numbers of leucine rich repeat motifs in tested TLRs. In conclusion, for animals infected with endometritis, there was a significant difference of TLR2 and TLR4 in uterine tissue compared to uninfected animals. Therefore, it is suggested to consider the TLRs results as detection markers for endometritis.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization and Transcriptional Profile of Toll-Like Receptors of Bubalus bubalis with Endometritis

Osman¹

2015

J. Inf. Mol. Biol.

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“…There are ~ 13 known mammalian TLRs: TLRs 1-13 in murine animals (Hopkins and Sriskandan 2005), TLRs 1-10 (except TLR7) in rabbits (Chen et al 2014), TLRs 1-10 in ruminants (Menzies and Ingham 2006;Chang et al 2009;Raja et al 2011), and TLRs 1-10 in humans (Rock et al 1998;Werling and Coffey 2007). The full sequence of TLR genes has been discovered in humans, monkeys, mice, rats, rabbits, pigs, cattle, sheep, goats, horses and chicken (Rock et al 1998;Yilmaz et al 2005 genes of Indian river type NR buffaloes have been cloned and sequenced (Dubey et al 2013). However, TLR gene sequences of swamp-type buffaloes from GX region in China have not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

De novo characterization of the genetic polymorphism and transcript abundance of Toll-like receptors (TLRs) in tissues of swamp buffaloes (Bubalus bubalis) from Guangxi, China

Shi

Mei

Kang

et al. 2020

Preprint

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Background: Toll-like receptors (TLRs) are innate signaling receptors, which sense invading pathogens and play roles in induction of immune responses to counter infection. In this study, we for the first time, cloned, sequenced and characterized the coding sequences of TLRs 1-10 in the Chinese “swamp-type” Guangxi (GX) buffalo breed. Results: Protein sequence analysis of the 10 TLRs showed that GX buffalo share significant sequence similarity with river buffalo (94.7 to 99.9%) and other ruminants (89.6% to 99%), but similarity with non-ruminants was low (48% to 91%). Prediction of functional domains revealed the presence of Toll/IL-1 receptor domains and variable numbers of leucine-rich repeats (LRRs) in all TLRs of GX buffalo. Transmembrane domain and signal peptide were absent in TLRs 7, 9 and TLRs 1, 3, 9, 10, respectively. When comparing GX buffalo and Nili-Ravi (NR) buffalo, the similarity of TIR domains ranged from 95.9% to 100%, while variations were found in the number of LRR motifs between these two breeds. Phylogenetic analysis based on the coding DNA and protein sequences demonstrated that, GX buffalo and NR buffalo were closely related with respect to TLRs 1, 2, 4, 5, 7, 8 and 10. However, regarding TLRs 3, 6, and 9, GX buffalo clustered with genera Bos and Bison, but not NR buffalo. RT-qPCR analysis provided an overview of TLRs transcription in tissues of GX buffalo. Conclusions: Our study obtained and characterized the full sequences of 10 TLR genes of GX buffaloes, and comparatively quantified the mRNA expression of these genes across various tissues of GX buffaloes. These findings lay the groundwork for discovering breed-specific differences in the occurrence and progression of infections in buffalo.

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Sequence-based structural analysis and evaluation of polymorphism in buffalo Nod-like receptor-1 gene

et al. 2019

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In this study, we have sequence characterized and analyzed the polymorphism in buffalo NOD1 (nucleotide-binding oligomerization domain 1) gene as well as its expression analysis. Full-length sequence analysis of NOD1 revealed this gene in buffalo being conserved with respect to the domain structures, similar to other species. Alternate splice variants having exon3 skipping also identified for the first time in the gene expressed in buffalo-purified peripheral blood mononuclear cells (PBMCs). Phylogenetically ruminant species were found to be clustering together and buffalo displaying maximum similarity with cattle. Sequencing of NOD1 across 12 Indian buffalo breeds identified 23 polymorphic sites within coding region, among which 16 were synonymous and 7 changes found to be non-synonymous. Four SNPs (single nucleotide polymorphisms) of them were genotyped in 393 animals belonging to 12 riverine, swamp and hybrid (riverine × swamp) buffalo populations of diverse phenotypes and utilities, showing variable allelic frequencies. Principal component analysis revealed, riverine and swamp buffaloes being distinctly placed with the distribution of breeds within the group based on the geographical isolation. Further, quantitative real-time PCR detected NOD1 expression in multiple tissues with PBMCs and lungs showing highest expression among the tissues examined. Structural analysis based on the translated amino acid sequence of buffalo NOD1 identified four protein interaction motifs LxxLL important for ligand binding. Molecular interaction analysis of iE-DAP and NOD1-LRR and their complex stability and binding-free energy studies indicated variable binding energies in buffalo and cattle NOD1. Overall, the study reveals unique structural features in buffalo NOD1, important for species-specific ligand interaction.

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Sequence characterization of river buffalo Toll‐like receptor genes 1–10 reveals distinct relationship with cattle and sheep

Cited by 14 publications

References 26 publications

Characterization and Transcriptional Profile of Toll-Like Receptors of Bubalus bubalis with Endometritis

Characterization and Transcriptional Profile of Toll-Like Receptors of Bubalus bubalis with Endometritis

De novo characterization of the genetic polymorphism and transcript abundance of Toll-like receptors (TLRs) in tissues of swamp buffaloes (Bubalus bubalis) from Guangxi, China

Sequence-based structural analysis and evaluation of polymorphism in buffalo Nod-like receptor-1 gene

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