Detection of Polymorphism at the Insulin Like Growth Factor-I Gene in Mazandaran Native Chicken using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method

Abstract

Problem statement: Molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in chickens. The Insulin like Growth Factor-I (IGF1) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. Approach: In the present study polymorphism of the promoter and 5' untranslated region of IGF-1 gene of Mazandaran native fowls was investigated. In order to evaluate the IGF-1 gene polymorphism we have used a Restriction Fragment Length Polymorphism (RFLP) method. Blood samples were collected from randomly chosen 100 Mazandaran native fowls. Genomic DNA was extracted using modified salting-out method and used amplified polymerase chain reaction technique. The promoter and 5' untranslated region of the fowl IGF-1 gene was amplified to produce a 621 bp fragment. The PCR products were electrophoresed on 2.5% agarose gel and stained by etidium bromide. Results: Then, they were digested of amplicon with PstI and revealed two alleles A and B. Data were analyzed using Pop Gene 32 package. In this population, AA, AB, BB genotype have been identified with the 25.88, 50.23, 23.89% frequencies. A and B alleles frequencies were 0.51, 0.49, respectively. The Chi-square (χ 2 ) test was significant and the population was in Hardy-Weinberg equilibrium (p<0.05). Conclusion: The PCR technique amplified a DNA fragment of IGF-1 with 621 bp. The results of the RFLP analysis showed two fragment 257 and 354bp after restriction with enzyme with PstI that identify changes in 5′ untranslated region. In according to action modes and importance of IGF-1, its polymorphisms can be related to economical traits such as body weight, muscle cells and bone.

Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics

“…It is demonstrated by the unrestricted fragment using the restriction enzyme Pst1. It is in line with previous results which stated that unrestricted DNA fragments define homozygote alleles (Nagaraja et al 2000;Kim et al 2004;Abbasi and Kazemi 2011;Kadlec et al 2011;Ali et al 2016). It also supported by Mu'in et al (2009), who reported that the restriction enzyme would be unable to cut the DNA fragment within homozygote alleles.…”

“…IGF-1 has a role in supporting the growth of somatic cells, fibroblasts, muscle, and bone (Abbasi and Kazemi 2011). Therefore, this gene is also involved in chicken growth.…”

Effects of genetic polymorphisms on egg production in indigenous Noi chicken

“…For example, on Ningdu Shanghuang breed, the frequency of C allele was higher than T allele in DRD2/BSeGI mutation point (Xu et al, 2010;Xu et al, 2011a;Xu et al, 2011b). In addition, Kim et al (2004) showed that C allele frequency (0.31) was lower than T allele (0.69) at IGF-I/PstI polymorphic position in Ogol chicken, but on the same Madandaran chicken, the distribution of 2 alleles was relatively equal (0.51 and 0.49) (Abbasi & Kazemi, 2011). In addition, a comparison of allele frequency on VIPR-1/TaqI polymorphism also pointed out that most of the experimental chickens in the population had a higher proportion of C allele.…”

Polymorphism and expression of insulin-like growth factor 1 (IGF1) gene and its association with growth traits in chicken

“…Nagaraja et al (2000) showed that a PstI RFLP in the 5′ flanking region of the IGF1 gene was associated with egg and egg shell weights in a White Leghorn chicken population. Abbasi and Kazemi (2011) also reported a polymorphism in the 621 bp flanking promoter and 5′ untranslated region in the fowl IGF1 gene and identified two alleles. A HinfI RFLP of a 793 bp fragment of the IGF1 gene was reported to be polymorphic in broiler chickens also indicating the presence of two alleles (Shah et al, 2012).…”