Detection of Platelet Aggregates with a Particle Counting Method Using Light Scattering

Biocompatible micellar nanovectors achieve efficient gene transfer to vascular lesions without cytotoxicity and thrombus formation

“…This instrument was reported to sensitively detect small aggregates consisting of only dozens of platelets formed under weak agonists. [36][37][38][39] The LSI was measured with the PA-200 to evaluate the existence and the extent of aggregates. According to the default configuration of the PA-200, the LSI was categorized to 'small', 'medium' and 'large' corresponding, respectively, to the small aggregates (9-25 mm), medium aggregates (26-50 mm) and large aggregates (51-70 mm).…”

Enhancement by cyclosporine A and tacrolimus of serotonin-induced formation of small platelet aggregation

“…[12][13][14][15][16] The principles of this method have been described previously. 12 A laser beam (675 nm) was passed through a platelet suspension, and the intensity of light scattering provided information on the number and size of aggregates. Data were recorded as a two-dimensional graph showing the change over time of total light intensity expressed as cumulative summation.…”

“…15 Therefore, weak aggregation is observed as a reaction in which only small aggregates are formed, and small aggregates are fused to large aggregates as the grade of platelet activation strengthens. 12,16 In platelet aggregation by a strong agonist, such as thrombin, both small and large aggregates are simultaneously observed with a reaction start. It is thought that this method is more sensitive than the conventional method, and it is especially useful for observing a weak reaction.…”

“…It is thought that this method is more sensitive than the conventional method, and it is especially useful for observing a weak reaction. 12,15 PRP (180 l) was placed in a cuvette and incubated for 3 min at 37°C with 20 l of CsA or Tc solution (final concentration range, 0.1 to 10 g/ml) or Tyrode's solution for the control during rotation at 1000 r.p.m. Subsequently, 22 l of serotonin (final concentration, 1.0 m), or ADP (final concentration, 0.1 or 0.4 m) was added, and formation of small and large aggregates were monitored for 10 min.…”

“…However, slight activation of certain platelet function parameters, such as serotonin-induced platelet aggregation, has been difficult to detect using the conventional method of Born. 11 A recently developed method for studying platelet aggregation by a particle counting technique using light scattering [12][13][14][15][16] can detect subtle changes in platelet activation.…”