Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR

Hermsen, Cornelus C.; Telgt, Denise; Linders, Ellen H.P.; Locht, Louis A.T.F. van de; Eling, W.M.C.; Mensink, E. J. B. M.; Sauerwein, Robert W.

doi:10.1016/s0166-6851(01)00379-6

Cited by 225 publications

(198 citation statements)

References 21 publications

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“…A P. falciparum-specific 18S ribosomal DNA RT-PCR was performed on a CFX96™ real-time PCR detection system (Bio-Rad Laboratories, Hercules, CA) and a FAM-labeled Taqman probe as described. 16,19,20 If there were discordant results in duplicate samples (i.e., one positive result and one negative result), the RT-PCR for that sample was repeated. Results were not considered positive until they gave a signal in at least two of the duplicate samples.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Antigen Detection Tests, Microscopy, and Polymerase Chain Reaction for Diagnosis of Malaria in Peripheral Blood in Asymptomatic Pregnant Women in Nanoro, Burkina Faso

Kattenberg

Tahita²,

Versteeg³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Rapid diagnostics tests (RDTs) detect malaria specific antigen(s) in the circulation, even when parasites are sequestered in the placenta and not visible by microscopy. However, research on their diagnostic accuracy during pregnancy is limited. Pregnant women (n = 418) were screened for malaria during routine antenatal care by using two RDTs that detect histidine-rich protein 2 (HRP2) or Plasmodium lactate dehydrogenase, and enzyme-linked immunosorbent assays with antibodies that detect dihydrofolate reductase-thymidylate synthase or heme-detoxification protein, and compared with real-time polymerase chain reaction (RT-PCR) and microscopy for evaluation of their diagnostic accuracy. Prevalence of malaria infection was high (53% by PCR). The RT-PCR and the HRP2 RDT detected most cases of malaria during pregnancy, whereas microscopy, the Plasmodium lactate dehydrogenase RDT, and enzyme-linked immunosorbent assays for dihydrofolate reductase-thymidylate synthase and heme-detoxification protein antibodies did not detect several low-density infections. Therefore, the HRP2 RDT could be a useful tool in high-transmission areas for diagnosis of malaria in asymptomatic pregnant women.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Antigen Detection Tests, Microscopy, and Polymerase Chain Reaction for Diagnosis of Malaria in Peripheral Blood in Asymptomatic Pregnant Women in Nanoro, Burkina Faso

Kattenberg

Tahita²,

Versteeg³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…Although molecular diagnostic assays that varied somewhat in protocol had been previously performed on samples for detection of Pf infection, the assays were repeated using the same molecular diagnostic test on samples performed at the same time to assure consistency. 12,15,18 Rather than repeating an assay on all samples from this cohort, approximately one-half of the available samples were examined for all species of malaria using the described protocol (PCR-LDR). Concordance for Pf infection comparing results obtained in both assays (real-time quantitative PCR [RTQ-PCR] and PCR-LDR) was approximately 86% (data not shown).…”

Section: Studymentioning

confidence: 99%

Marked Decline in Malaria Prevalence among Pregnant Women and Their Offspring from 1996 to 2010 on the South Kenyan Coast

Kalayjian

Malhotra

Mungai

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Expanded malaria control in Kenya since the early 2000s has resulted in marked reduction in hospital admissions for malaria; however, no studies have reported changes in malaria infection rates in the same population over this period. Randomly selected archived blood samples from four cohorts of pregnant women and their children from 1996 to 2010 in Kwale District, Coast Province, Kenya, were examined for Plasmodium falciparum (Pf ), P. malariae, P. ovale, and Plasmodium vivax by quantitative polymerase chain reaction (PCR) and microscopy. Maternal delivery Pf prevalence by PCR declined from 40% in 2000-2005 to 1% in 2009-2010, concordant with increased bed net and malaria chemoprophylaxis use. Individual risk of Pf infection in children from birth to 3 years in serial longitudinal cohort studies declined from almost 100% in 1996-1999 to 15% in 2006-2010. Declines in P. malariae and P. ovale infections rates were also observed. These results show a profound reduction in malaria transmission in coastal Kenya. BACKGROUND

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“…Furthermore, it is becoming clear that innate responses can differ between individuals (25,36) with potential clinical implications. To date, only two-rather small-studies have exploited the unique opportunity offered by experimental infection of malaria-naive volunteers (during the evaluation of antimalarial vaccines) to study the very early stages of the immune response to malaria (37,38), and it has been difficult to draw any generalizable conclusions from them. Here, we present a detailed longitudinal analysis of 11 different cytokine responses in 18 experimentally infected individuals, using a highly sensitive, bead-based multiplex assay, and we compare these responses with peripheral parasite densities, as measured by quantitative real-time PCR (39).…”

Section: R Esearch On the Immunology Of Malaria Infection Has Beenmentioning

confidence: 99%

Innate Immune Responses to Human Malaria: Heterogeneous Cytokine Responses to Blood-Stage Plasmodium falciparum Correlate with Parasitological and Clinical Outcomes

Walther

Woodruff

Edele

et al. 2006

The Journal of Immunology

141

118

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Taking advantage of a sporozoite challenge model established to evaluate the efficacy of new malaria vaccine candidates, we have explored the kinetics of systemic cytokine responses during the prepatent period of Plasmodium falciparum infection in 18 unvaccinated, previously malaria-naive subjects, using a highly sensitive, bead-based multiplex assay, and relate these data to peripheral parasite densities as measured by quantitative real-time PCR. These data are complemented with the analysis of cytokine production measured in vitro from whole blood or PBMC, stimulated with P. falciparum-infected RBC. We found considerable qualitative and quantitative interindividual variability in the innate responses, with subjects falling into three groups according to the strength of their inflammatory response. One group secreted moderate levels of IFN-γ and IL-10, but no detectable IL-12p70. A second group produced detectable levels of circulating IL-12p70 and developed very high levels of IFN-γ and IL-10. The third group failed to up-regulate any significant proinflammatory responses, but showed the highest levels of TGF-β. Proinflammatory responses were associated with more rapid control of parasite growth but only at the cost of developing clinical symptoms, suggesting that the initial innate response may have far-reaching consequences on disease outcome. Furthermore, the in vitro observations on cytokine kinetics presented here, suggest that intact schizont-stage infected RBC can trigger innate responses before rupture of the infected RBC.

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Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR

Cited by 225 publications

References 21 publications

Evaluation of Antigen Detection Tests, Microscopy, and Polymerase Chain Reaction for Diagnosis of Malaria in Peripheral Blood in Asymptomatic Pregnant Women in Nanoro, Burkina Faso

Evaluation of Antigen Detection Tests, Microscopy, and Polymerase Chain Reaction for Diagnosis of Malaria in Peripheral Blood in Asymptomatic Pregnant Women in Nanoro, Burkina Faso

Marked Decline in Malaria Prevalence among Pregnant Women and Their Offspring from 1996 to 2010 on the South Kenyan Coast

Innate Immune Responses to Human Malaria: Heterogeneous Cytokine Responses to Blood-Stage Plasmodium falciparum Correlate with Parasitological and Clinical Outcomes

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