Detection of plasmid-mediated AmpC β-lactamase in Escherichia coli and Klebsiella pneumoniae

Abstract

These results emphasize that clinical laboratories should consider testing the presence of pAmpC enzymes particularly in FOX-resistant isolates, and BA disk test will improve detection of this emerging resistance phenotype.

Molecular determinants of ß-lactamase producing Klebsiella pneumoniae in MansouraUniversity Neonatal Intensive Care Unit

“…The study of Al-Agamy (28) in Egypt, reported less prevalence rate of bla CMY-2 (5%) and slightly more rate of bla DHA (23.5%). Furthermore, a second Egyptian study by the same author demonstrated bla CMY , as a sole gene among AmpCß-lactamase genes in the Klebsiella isolates (29), whereas a Turkish study has found that all their K. pneumoniae isolates carried MOX group genes but bla DHA and bla ACC genes were not detected (30). All these results confirm the fact that these ESBL types are widely different within the same and each country.…”

AmpC and Extended Spectrum Beta Lactamase Producing Isolates of E. coli, Klebsiella spp. and P. mirabilis in a Tertiary Care Center and their Sensitivity to Other Antibiotics

“…Despite this fact, coproduction of AmpC and ESBLs has been documented in many studies (Grover et al, 2013;Doi and Paterson, 2007;Bakthavatchalu et al, 2013;Mohanty et al, 2010). Prevalence of AmpC producers have been reported to be from 8% to as high as 41.3% in various studies from India and from 1.15% to as high as 46.7% in various studies from other countries (Peter-Getzlaff et al, 2011;Grover et al, 2013;Bakthavatchalu et al, 2013;Polsfuss et al, 2011;Li et al, 2003;Singhal et al, 2005;Manoharan et al, 2012;Maraskolhr et al, 2014;Rudresh and Nagarathnamma, 2011;Tan et al, 2009;Helmy and Wasfi, 2014;Ingram et al, 2011;Yilmaz et al, 2013;Coudron, 2005).…”

“…Yilmaz et al,reported 39.56% isolates to be positive by disc potentiation test including 48.69% and 18.29% E. coli and K. pneumoniae respectively (Yilmaz et al, 2013). Manoharan et al reported 12.5% isolates to be AmpC producers by disc potentiation test (Manoharan et al, 2012 (Bakthavatchalu et al, 2013).…”

“…Yilmaz et al, reported significant resistance against ceftazidime, cefotaxime and amoxycillin+ clavulanic acid in case of AmpC producers, (Yilmaz et al, 2013). Grover et al, reported a significant resistance against gentamicin, third generation cephalosporins, cefepime and fluoroquinolones by β-lactamase producers (Grover et al, 2013).…”

Detection of Inducible and Non-inducible (constitutive) AmpC β-lactamase-producing Gram-Negative Bacteria among Family Enterobacteriaceae by Two Phenotypic Methods-Disk Antagonism Test (DAT) and AmpC disk Test at a Tertiary Care Hospital, Himachal Pradesh, India

“…In all these AmpC producers, we were not able to distinguish between the chromosomal and plasmid mediated enzymes, as this requires genotypic confirmatory tests. (Yilmaz et al, 2007;Doi and Paterson, 2007) Figure.1.Disk Antagonism Test (DAT)…”