Detection of peptidic erythropoiesis-stimulating agents in sport

Reichel, Christian

doi:10.1136/bjsports-2014-093555

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…Despite its suspected, purported, and proven misuse in elite sport, the debate as to its utility in different clinical settings, performance enhancing properties, and modus operandi of increasing endurance in healthy individuals is ongoing . Unaffected, the need to test for xenobiotic EPO, to distinguish unambiguously between endogenous and exogenous origin of the EPO and to improve test methods to cope with trends in doping practices, such as repetitive microdosing is respected and timely reviews summarize recent developments and accomplishments as well as future goals in a comprehensive manner …”

Section: Peptide Hormones Growth Factors and Related Substancesmentioning

confidence: 99%

Annual banned‐substance review: analytical approaches in human sports drug testing

Thevis

Kuuranne

Geyer

et al. 2014

Drug Testing and Analysis

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Within the mosaic display of international anti-doping efforts, analytical strategies based on up-to-date instrumentation as well as most recent information about physiology, pharmacology, metabolism, etc., of prohibited substances and methods of doping are indispensable. The continuous emergence of new chemical entities and the identification of arguably beneficial effects of established or even obsolete drugs on endurance, strength, and regeneration, necessitate frequent and adequate adaptations of sports drug testing procedures. These largely rely on exploiting new technologies, extending the substance coverage of existing test protocols, and generating new insights into metabolism, distribution, and elimination of compounds prohibited by the World Anti-Doping Agency (WADA). In reference of the content of the 2014 Prohibited List, literature concerning human sports drug testing that was published between October 2013 and September 2014 is summarized and reviewed in this annual banned-substance review, with particular emphasis on analytical approaches and their contribution to enhanced doping controls.

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Section: Peptide Hormones Growth Factors and Related Substancesmentioning

confidence: 99%

Annual banned‐substance review: analytical approaches in human sports drug testing

Thevis

Kuuranne

Geyer

et al. 2014

Drug Testing and Analysis

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“…For this reason, rEPO is included in the list of prohibited substances and doping methods, annually updated by the World Anti-Doping Agency (WADA) (World Anti-doping Agency, 2023). At present, the detection of the abuse of rEPO in doping control is OPEN ACCESS EDITED BY performed, in urine and plasma, by immunoelectrophoresis, using Western Blot originally preceded by polyacrylamide gel isoelectric focusing (IEF-PAGE), and more recently by either N-lauroysarcosinate SAR-polyacrylamide gel (SAR-PAGE), or sodium dodecyl sulfate, gel electrophoresis (SDS-PAGE) (Jelkmann and Lundby, 2011;Reichel, 2014). Hence, the presence of rEPO in a sample can be detected due to the difference in the molecular mass compared to the endogenous erythropoietin isoform (Pascual et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Detection of exon 5 c.577del variant of human erythropoietin gene in whole blood, dried blood spots and urine samples for doping control

et al. 2023

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Background: In doping control, the presence of the exon5 c.577del variant in the human erythropoietin gene may be a confounding factor in the interpretation of the results from the analytical method currently in force for the detection of human recombinant erythropoietin, based on immunoelectrophoresis on SDS-PAGE and/or SAR-PAGE. This variant, determining the transcription of a higher molecular weight protein, can erroneously suggest the presence of recombinant erythropoietin in a biological sample, causing the possibility of a false positive result. Although the variant was now identified only in East Asian populations and with a very low frequency, it can threaten the reliability of current anti-doping tests.Methods: We have implemented a genetic test to identify the presence of this variant in the biological samples that are presently collected for anti-doping analysis (whole blood, urine, and dried blood spots). The test is based on the Sanger sequencing of the human erythropoietin gene exon 5, where the c.577del variant falls.Results and Discussion: The method has a specificity of 100% and allows identification of the possible presence of the variant starting from 100 pg of genomic DNA extracted from each biological sample. The efficacy of the test has been confirmed by the analysis of real samples from subjects showing and not showing the exon c.577del variant.

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Reduction of hemoglobin, not iron, inhibited maturation of red blood cells in male rats exposed to high intensity endurance exercises

Tang

et al. 2019

Journal of Trace Elements in Medicine and Biology

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Detection of peptidic erythropoiesis-stimulating agents in sport

Cited by 5 publications

References 37 publications

Annual banned‐substance review: analytical approaches in human sports drug testing

Annual banned‐substance review: analytical approaches in human sports drug testing

Detection of exon 5 c.577del variant of human erythropoietin gene in whole blood, dried blood spots and urine samples for doping control

Reduction of hemoglobin, not iron, inhibited maturation of red blood cells in male rats exposed to high intensity endurance exercises

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