Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11

Kim, Junyoung; Sahu, Shovan Kumar; Yau, Y. K.; Wang, Xu; Shochat, Susana Geifman; Nielsen, Per Halkjær; Dueholm, Morten Simonsen; Otzen, Daniel E.; Lee, Jungyeol; Santos, May Margarette Salido Delos; Yam, Joey Kuok Hoong; Kang, Nam‐Young; Park, Sung-Jin; Kwon, Hyeokjin; Seviour, Thomas; Yang, Liang; Givskov, Michael; Chang, Young‐Tae

doi:10.1021/jacs.5b11357

Cited by 83 publications

(60 citation statements)

References 26 publications

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“…Using the fluorescent probe CDy11, which targets amyloid fibrils, we observed that this biofilm matrix component was detected more abundantly in our biofilm preparations than in the samples of planktonic bacteria ( Fig. 1c-d) [9].…”

Section: Preparation and Characterization Of Calibrated Inocula Of Stmentioning

confidence: 79%

“…For 24 h-old biofilm cultures, 700 μL of supernatant were carefully withdrawn from the cell culture plates before homogenization of the remaining suspension. A 10 μM stock solution of the fluorescent probe CDy11 [9] was prepared in dimethyl sulfoxide (DMSO). The solution was diluted in phosphatebuffered saline solution (PBS) to prepare a 100 μM solution.…”

Section: Detection Of Amyloid Fibrils In Biofilm Preparationsmentioning

confidence: 99%

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A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms

Hamid¹,

Nakusi²,

Givskov³

et al. 2020

BMC Microbiol

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Background: Staphylococcus aureus is a human pathogen that is a common cause of nosocomial infections and infections on indwelling medical devices, mainly due to its ability to shift between the planktonic and the biofilm/ sessile lifestyle. Biofilm infections present a serious problem in human medicine as they often lead to bacterial persistence and thus to chronic infections. The immune responses elicited by biofilms have been described as specific and ineffective. In the few experiments performed in vivo, the importance of neutrophils and macrophages as a first line of defence against biofilm infections was clearly established. However, the bilateral interactions between biofilms and myeloid cells remain poorly studied and analysis of the dynamic processes at the cellular level in tissues inoculated with biofilm bacteria is still an unexplored field. It is urgent, therefore, to develop biologically sound experimental approaches in vivo designed to extract specific immune signatures from the planktonic and biofilm forms of bacteria. Results: We propose an in vivo transgenic mouse model, used in conjunction with intravital confocal microscopy to study the dynamics of host inflammatory responses to bacteria. Culture conditions were created to prepare calibrated inocula of fluorescent planktonic and biofilm forms of bacteria. A confocal imaging acquisition and analysis protocol was then drawn up to study the recruitment of innate immune cells in the skin of LysM-EGFP transgenic mice. Using the mouse ear pinna model, we showed that inflammatory responses to S. aureus can be quantified over time and that the dynamics of innate immune cells after injection of either the planktonic or biofilm form can be characterized. First results showed that the ability of phagocytic cells to infiltrate the injection site and their motility is not the same in planktonic and biofilm forms of bacteria despite the cells being considerably recruited in both cases. Conclusion:We developed a mouse model of infection to compare the dynamics of the inflammatory responses to planktonic and biofilm bacteria at the tissue and cellular levels. The mouse ear pinna model is a powerful imaging system to analyse the mechanisms of biofilm tolerance to immune attacks.

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Section: Preparation and Characterization Of Calibrated Inocula Of Stmentioning

confidence: 79%

Section: Detection Of Amyloid Fibrils In Biofilm Preparationsmentioning

confidence: 99%

A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms

Hamid¹,

Nakusi²,

Givskov³

et al. 2020

BMC Microbiol

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“…The binding capability of peptides endows the target ability to different tissues and lesions, including bones, muscles, vascular, solid tumors, and bacteria‐infected location, etc. The Glu 6 and Asp 6 can bind to the bone through interactions with hydroxyapatite.…”

Section: Functional Peptides and Driving Forces For Self‐assemblymentioning

confidence: 99%

Self‐Assembled Peptide‐Based Nanomaterials for Biomedical Imaging and Therapy

et al. 2018

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Peptide-based materials are one of the most important biomaterials, with diverse structures and functionalities. Over the past few decades, a self-assembly strategy is introduced to construct peptide-based nanomaterials, which can form well-controlled superstructures with high stability and multivalent effect. More recently, peptide-based functional biomaterials are widely utilized in clinical applications. However, there is no comprehensive review article that summarizes this growing area, from fundamental research to clinic translation. In this review, the recent progress of peptide-based materials, from molecular building block peptides and self-assembly driving forces, to biomedical and clinical applications is systematically summarized. Ex situ and in situ constructed nanomaterials based on functional peptides are presented. The advantages of intelligent in situ construction of peptide-based nanomaterials in vivo are emphasized, including construction strategy, nanostructure modulation, and biomedical effects. This review highlights the importance of self-assembled peptide nanostructures for nanomedicine and can facilitate further knowledge and understanding of these nanosystems toward clinical translation.

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“…Fluorescence spectroscopy is probably the most widely used technique in bio‐chemical sensing technology . In situ quantitative determination of exogenous or native fluorophores in tissue by fluorescence spectroscopy has the potential to aid medical diagnoses .…”

Section: Applications Of Ssd In Quantitative Analysis Of Complex Systemsmentioning

confidence: 99%

“…Because of their high measuring speed and requirement of a lesser amount of, or even no, sample preparation, spectroscopic techniques such as near‐infrared (NIR) spectroscopy, Raman spectroscopy, and fluorescence spectroscopy have been widely applied to quantitative analysis of complex samples in fields such as chemical and pharmaceutical processes , biomedical sciences, food safety, environmental protection, as well as clinic diagnosis, etc . In quantitative analysis of samples using spectroscopic techniques, calibration models are needed to transform spectroscopic measurements into the desired concentration information.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis based on spectral shape deformation: A review of the theory and its applications

Chen

Xiong

Zuo

et al. 2017

Journal of Chemometrics

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Most of the commonly used calibration methods in quantitative spectroscopic analysis are established or derived from the assumption of a linear relationship between the concentrations of the analytes of interest and corresponding absolute spectral intensities. They are not applicable for heterogeneous samples where the potential uncontrolled variations in optical path length due to the changes in samples' physical properties undermine the basic assumption behind them. About a decade ago, a unique calibration strategy was proposed to extract chemical information from spectral data contaminated with multiplicative light scattering effects. From then on, this calibration strategy has been attentively examined, modified, and used by its developers. After more than 10 years of development, some important features of the calibration strategy have been identified. It has been proved that the calibration strategy can solve many complex problems in quantitative spectroscopic analysis. But, because of the relatively low awareness of the calibration strategy among chemometrics society, its potential has not been fully exploited yet. This paper reviews the theory of the calibration strategy and its applications with a view to introducing the unique and powerful calibration strategy to a wider audience.

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Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11

Cited by 83 publications

References 26 publications

A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms

A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms

Self‐Assembled Peptide‐Based Nanomaterials for Biomedical Imaging and Therapy

Quantitative analysis based on spectral shape deformation: A review of the theory and its applications

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