Detection of Panton–Valentine leukocidin-positive livestock-associated Staphylococcus aureus CC398 clone in a Vietnamese patient in Japan

Nakaminami, Hidemasa; Miyata, Takeo; Yokomori, Shiho; Fusegawa, Takehito; Murata, Shinichi; Noguchi, Norihisa

doi:10.1016/j.jgar.2020.08.010

Cited by 2 publications

(1 citation statement)

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“…All sequenced PVL-positive GG0398 isolates also carried the ϕSa3 phage carrying sak and scn genes. Recently, Japanese researchers reported two patients from whom they isolated PVL-positive CC398 isolates with ST1232 that carried the sak and chp genes 33 , 34 . Shortly thereafter, an ST1232 isolate from a Korean patient was studied in a detailed comparison with the isolates from Japan 35 .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of MRSA collected during national surveillance between 2008 and 2019 in the Netherlands

Schouls,

Witteveen,

van Santen-Verheuvel

et al. 2023

Commun Med

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Background. Although the Netherlands is a country with a low endemic level, methicillin-resistant Staphylococcus aureus (MRSA) poses a significant health care problem. Therefore, high coverage national MRSA surveillance has been in place since 1989. To monitor possible changes in the type-distribution and emergence of resistance and virulence, MRSA isolates are molecularly characterized. Methods. All 43,321 isolates from 36,520 persons, collected 2008–2019, were typed by multiple-locus variable number tandem repeats analysis (MLVA) with simultaneous PCR detection of the mecA, mecC and lukF-PV genes, indicative for PVL. Next-generation sequencing data of 4991 isolates from 4798 persons were used for whole genome multi-locus sequence typing (wgMLST) and identification of resistance and virulence genes. Results. We show temporal change in the molecular characteristics of the MRSA population with the proportion of PVL-positive isolates increasing from 15% in 2008–2010 to 25% in 2017–2019. In livestock-associated MRSA obtained from humans, PVL-positivity increases to 6% in 2017–2019 with isolates predominantly from regions with few pig farms. wgMLST reveals the presence of 35 genogroups with distinct resistance, virulence gene profiles and specimen origin. Typing shows prolonged persistent MRSA carriage with a mean carriage period of 407 days. There is a clear spatial and a weak temporal relationship between isolates that clustered in wgMLST, indicative for regional spread of MRSA strains. Conclusions. Using molecular characterization, this exceptionally large study shows genomic changes in the MRSA population at the national level. It reveals waxing and waning of types and genogroups and an increasing proportion of PVL-positive MRSA.

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Section: Discussionmentioning

confidence: 99%