Comparative Genomic Analysis of the Human Variant of Methicillin-Resistant <i>Staphylococcus aureus</i> CC398 in Japan and Korea

Kaneko, Hiroshi; Kim, Eu Suk; Yokomori, Shiho; Moon, Song Mi; Song, Kyoung-Ho; Jung, Jongtak; Park, Jeong Su; Kim, Hong Bin; Nakaminami, Hidemasa

doi:10.1089/mdr.2021.0203

Cited by 4 publications

(6 citation statements)

References 33 publications

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“…Two CC22 strains carried both PVL and TSST-1 genes. The PFGE patterns of the CC398 strains were not detected because of their resistance to Sma I digestion ( 41 ).…”

Section: Resultsmentioning

confidence: 99%

“…The ST1232 strains are considered to be the human-associated clade of CC398 MRSA, of which the livestock-associated clade is widely known ( 52 ). This CC398 clone has spread across Southeast Asia and has recently emerged in Japan ( 41 ). Although the PVL-positive CC22, CC1, CC59, and CC398 strains exhibited different genotypic and phenotypic proﬁles from the PVL-positive CC8 strains, especially those lacking ACME genes ( Fig.…”

Section: Discussionmentioning

confidence: 99%

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Significant increase in the prevalence of Panton–Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus , particularly the USA300 variant ΨUSA300, in the Japanese community

Kaneko,

Kanai,

Saito

et al. 2023

Microbiol Spectr

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USA300, a major Panton–Valentine leukocidin (PVL)-producing methicillin-resistant Staphylococcus aureus (MRSA) clone associated with skin and soft tissue infections (SSTIs), carries a type IV staphylococcal cassette chromosome mec (SCC mec ) element. Recently, a USA300 variant, ΨUSA300, with a 12-bp deletion of ccrB2 in the SCC mec element, was identified in Japan by Takadama et al. (15). To understand the current status of PVL-positive MRSA in the community, a nationwide surveillance of MRSA isolated from outpatients at dermatology departments was conducted. This study included 1,619 outpatients who visited dermatology departments at 22 healthcare facilities in 11 prefectures in Japan between 2018 and 2021; S. aureus were isolated from these outpatients. Molecular epidemiological analysis was performed using PCR, SCC mec and spa typing, pulsed-field gel electrophoresis, multilocus sequence typing, and antimicrobial susceptibility testing. A total of 980 S . aureus were isolated, 293 of which were identified as MRSA. Among MRSA isolates, PVL genes were detected in 18 of 63 (28.6%) in 2018, 34 of 85 (40.0%) in 2019, 29 of 65 (44.6%) in 2020, and 40 in 80 (50.0%) in 2021. Among the PVL-positive MRSA, 16 of 121 (13.2%) and 94 of 121 (77.7%) isolates were identified as USA300 and ΨUSA300, respectively. ΨUSA300 strains were collected from six prefectures in Japan: Kagawa, Osaka, Tokyo, Kanagawa, Fukushima, and Saitama. ΨUSA300 was isolated more frequently than USA300 from patients with deep-seated SSTIs ( P < 0.05). Our findings showed that PVL-positive MRSA isolates were significantly increased in the Japanese community. Furthermore, the USA300 variant ΨUSA300 has emerged as the predominant PVL-positive MRSA clone across the Kanto, Kinki, and Shikoku regions of Japan, and this variant may possess a greater propensity to cause deep-seated SSTIs than in USA300. IMPORTANCE USA300 is an MRSA clone producing PVL, a toxin associated with SSTIs. ΨUSA300 is a USA300 variant recently identified in Japan by Takadama et al. (15). Here, we found that the prevalence rate of PVL-positive MRSA in S. aureus was elevated in the Japanese community, and ΨUSA300 accounted for most of them. ΨUSA300 strains have been isolated from several areas in Japan and were associated with deep-seated SSTIs. This study highlighted the emerging threat posed by ΨUSA300 in Japan.

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“…Two CC22 strains carried both PVL and TSST-1 genes. The PFGE patterns of the CC398 strains were not detected because of their resistance to Sma I digestion ( 41 ).…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Significant increase in the prevalence of Panton–Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus , particularly the USA300 variant ΨUSA300, in the Japanese community

Kaneko,

Kanai,

Saito

et al. 2023

Microbiol Spectr

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“…MLST showed sequence types (STs) belonging to CC398, including ST1232 (n = 2), a single-locus variant of ST398, and ST4081 (n = 1), a double-locus variant of ST398. WGS and comparative genomic analysis of SNPs confirmed that all isolates were part of the same L2 lineage within the II-GOI clade ( Figure ) ( 1 – 6 ). We performed cgMLST on 1,861 genes, and results showed each patient had a different complex type (CT), CT6700, CT6814, and CT7459, thus ruling out direct transmission events between the patients.…”

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confidence: 82%

“…Clonal complex 398 (CC398) is of particular concern because it includes common livestock-associated methicillin-resistant S. aureus (LA-MRSA) IIa subclades in Europe and human-origin MRSA (HO-MRSA) II-GOI subclades in the Asia-Pacific Region ( 1 – 4 ). Panton-Valentine leukocidin (PVL)–positive HO-MRSA L2 strains have recently emerged and are expanding geographically, causing skin and soft-tissue infections and occasional invasive disease in humans ( 2 , 3 , 5 , 6 ).…”

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confidence: 99%

Panton-Valentine Leukocidin–Positive CC398 MRSA in Urban Clinical Settings, the Netherlands

Gooskens¹,

Konstantinovski²,

Kraakman³

et al. 2023

Emerg. Infect. Dis.

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We report detection of Panton-Valentine leukocidin–positive clonal complex 398 human-origin methicillin-resistant Staphylococcus aureus L2 in the Netherlands. This hypervirulent lineage originated in the Asia-Pacific Region and could become community-acquired in Europe after recurrent travel-related introductions. Genomic surveillance enables early detection to guide control measures and help limit spread of pathogens in urban settings.

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“…CC398 MRSA strains usually exhibit decreased virulence compared with other MRSA lineages due to a limited number of virulence-associated determinants. However, highly virulent CC398 MRSA strains carrying risk factors such as Panton-Valentine leucocidin (PVL) and immune evasion cluster (IEC) genes have recently emerged in hospital and community settings [2,3]. Here, we report the genomic information of two CC398 MRSA clinical isolates (SA489 and SA497).…”

mentioning

confidence: 99%

Complete Genome Sequences of Two Clonal Complex 398 Methicillin-resistant Staphylococcus aureus Strains Isolated from Patients in Korea

Lee

Park

Lim

et al. 2023

Microbiol. Biotechnol. Lett.

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Comparative Genomic Analysis of the Human Variant of Methicillin-Resistant Staphylococcus aureus CC398 in Japan and Korea

Cited by 4 publications

References 33 publications

Significant increase in the prevalence of Panton–Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus , particularly the USA300 variant ΨUSA300, in the Japanese community

Significant increase in the prevalence of Panton–Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus , particularly the USA300 variant ΨUSA300, in the Japanese community

Panton-Valentine Leukocidin–Positive CC398 MRSA in Urban Clinical Settings, the Netherlands

Complete Genome Sequences of Two Clonal Complex 398 Methicillin-resistant Staphylococcus aureus Strains Isolated from Patients in Korea

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