Detection of p53 Common Intron Polymorphisms in Patients with Gastritis Lesions from Iran

Abstract

Background: p53 alterations have been implicated in the development of many cancers, such as gastric cancer, but there is no evidence of p53 intron alterations in gastritis lesions. The aim of this study was to investigate the p53 intron alterations in gastritis along with p53 and mismatch repair protein expression and microsatellite status. Materials and Methods: PCR-sequencing was conducted for introns 2-7 on DNA extracted from 97 paired samples of gastritis lesions and normal adjacent tissue. Abnormal accumulation of p53 and mismatch repair proteins was investigated using immunohistochemistry. In addition, microsatellite status was evaluated with reference to five mononucleotide markers. Results: Gastritis cases included 41 males and 56 females in the age range of 15-83 years, 87.6% being H.pylori positive. IVS2+38, IVS3ins16 and IVS7+72 were the most polymorphic sites. Their minor allele frequency values were as follows: 0.38, 0.21 and 0.06, respectively. Samples with GG genotype at IVS2+38 and CT at IVS7+72 had no insertion. Moreover, most of the stable samples (91.9 %) had a G allele at IVS2+38. All of the samples were IHC negative for p53 protein, microsatellite stable and expressed mismatch repair proteins. p53 alterations were prominent in the H. Pylori+ group, but without statistical significance. Conclusions: According to our results, some p53 polymorphisms such as IVS2+38, IVS3ins16 and IVS7+72, because of their correlations together or with microsatellite status may contribute to gastritis development. However, so far effects on p53 expression and function remain unclear. Therefore, a comprehensive survey is needed to delineate their biological significance.

Clinicopathological and p53 Gene Alteration Comparison between Young and Older Patients with Gastric Cancer

“…We had shown significant association of p53 expression and alterations with histological grades patients (Karim et al, 2009;Sadeghi et al, 2013). Alteration of p53 gene has been reported controversial with any age group.…”

Effects of p53 Codon 72 and MDM2 SNP309 Polymorphisms on Gastric Cancer Risk among the Iranian Population

“…Several epidemiological studies have evaluated the connection of p53 R72P, MDM2 SNP309 and risk of different types of cancer (Marine et al, 2006;Sadeghi et al, 2013;Song et al, 2013). In the current study the p53 R72P and MDM2 SNP309 polymorphisms were investigated in a series of 104 patients of GC, and in 100 population matched controls in order to verify the impact of P53 and MDM2 variants on the risk of tumor development in Iranian GC patients.…”

Association of Cytokine Gene Polymorphisms with Gastritis in a Kazakh Population

“…From another study exists evidence that key factor in development of gastric cancer p53 gene intron alterations may contribute gastritis development and early detection of these alterations in precancerous lesions such as gastritis may be useful for prediction of risk developing of gastric cancer (Najjar Sadeghi et al, 2013). Gastric cancer is partly a hereditary disease and according recently study the infection Helicobacter pylori and the stomach lesions such as dysplasia, atrophy and chronic gastritis have been met more frequently in individuals with a family history of gastric cancer (Mansour-Ghanaei et al, 2012).…”