Detection of organophosphate and pyrethroid resistance alleles in Czech <i>Leptinotarsa decemlineata</i> (Coleoptera: Chrysomelidae) populations by molecular methods

Zichová, Tereza; Kocourek, F.; Salava, J.; Nad'ová, Kateřina; Stará, Jitka

doi:10.1002/ps.1952

Cited by 21 publications

(22 citation statements)

References 26 publications

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“…In a recent paper Revuelta et al [46] found that the Colorado potato beetle has a second AChE gene that is homologous to the AChE1 of other insects which in some instance has been found to be the main target of OP selection [47]. Moreover, associations between the resistance S291G mutation and the resistant level of beetle populations have not been very strong [48,49]. Nevertheless, the site-directed mutagenesis has confirmed that the S291G mutation affects the sensitivity of the enzyme to OP insecticides [24] and thus the AChE 2 gene cannot be totally neutral.…”

Section: Discussionmentioning

confidence: 99%

Pre-invasion history and demography shape the genetic variation in the insecticide resistance-related acetylcholinesterase 2 gene in the invasive Colorado potato beetle

et al. 2013

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BackgroundInvasive pest species offers a unique opportunity to study the effects of genetic architecture, demography and selection on patterns of genetic variability. Invasive Colorado potato beetle (Leptinotarsa decemlineata) populations have experienced a rapid range expansion and intense selection by insecticides. By comparing native and invasive beetle populations, we studied the origins of organophosphate (OP) resistance-associated mutations in the acetylcholinesterase 2 (AChE2) gene, and the role of selection and demography on its genetic variability.ResultsAnalysis of three Mexican, two US and five European populations yielded a total of 49 haplotypes. Contrary to the expectations all genetic variability was associated with a point mutation linked to insecticide resistance (S291G), this mutation was found in 100% of Mexican, 95% of US and 71% of European beetle sequences analysed. Only two susceptible haplotypes, genetically very differentiated, were found, one in US and one in Europe. The genetic variability at the AChE2 gene was compared with two other genes not directly affected by insecticide selection, diapause protein 1 and juvenile hormone esterase. All three genes showed reduction in genetic variability indicative of a population bottleneck associated with the invasion.ConclusionsStochastic effects during invasion explain most of the observed patterns of genetic variability at the three genes investigated. The high frequency of the S291G mutation in the AChE2 gene among native populations suggests this mutation is the ancestral state and thus, either a pre-adaptation of the beetle for OP resistance or the AChE2 is not the major gene conferring OP resistance. The long historical association with host plant alkaloids together with recombination may have contributed to the high genetic variation at this locus. The genetic diversity in the AChE2 locus of the European beetles, in turn, strongly reflects founder effects followed by rapid invasion. Our results suggest that despite the long history of insecticide use in this species, demographic events together with pre-invasion history have been strongly influential in shaping the genetic diversity of the AChE2 gene in the invasive beetle populations.

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Section: Discussionmentioning

confidence: 99%

Pre-invasion history and demography shape the genetic variation in the insecticide resistance-related acetylcholinesterase 2 gene in the invasive Colorado potato beetle

et al. 2013

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“…Two point mutations in LdAChE2 have been associated with resistance (Ben'kovskaya et al, 2008;Kim et al, 2006a;Clark, 1995a, 1997;Zhu et al, 1996;Zichova et al, 2010). In addition, functional analysis by site-directed mutagenesis and baculovirus expression confirmed the influence of these mutations on the activity of the enzyme (Kim et al, 2007).…”

Section: Introductionmentioning

confidence: 88%

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle

Revuelta

Ortego

Díaz-Ruíz

et al. 2011

Insect Biochemistry and Molecular Biology

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The Colorado potato beetle (CPB), Leptinotarsa decemlineata is an important economic pest of potato worldwide. Resistance to organophosphates and carbamates in CPB has been associated in some cases to point mutations in the acetylcholinesterase (AChE) gene Ldace2, an orthologue of Drosophila melanogaster Dmace2. In this paper we report cloning and sequencing of Ldace1, an orthologue of Anopheles gambiae Agace1 that was previously unknown in CPB. The Ldace1 coding enzyme contains all residues conserved in a functionally active AChE. Ldace1 is expressed at higher levels (between 2- and 11-fold) than Ldace2 in embryos, in the four larval instars and in adults. Specific interference of Ldace1 by means of dsRNA injection resulted in a reduction of AChE activity to an approximate 50% compared to control, whilst interference of Ldace2 reduced AChE activity to an approximate 85%. Analysis of zymograms of AChE activity after interference indicates that LdAChE1 is the enzyme predominantly responsible for the activity visualised. Interference of Ldace1 in CPB adults caused a significant increase in mortality (43%) as early as three days post-injection (p.i.), suggesting the essential role of Ldace1. Interference of Ldace2 also caused a significant increase in mortality (29%) compared to control, although at seven days p.i. The effect of the interference of Ldace1 on susceptibility to the organophosphate chlorpyrifos points out that LdAChE1 could be a main target for this insecticide. In the light of our results, studies associating resistance in CPB to mutations in Ldace2 should be reviewed, taking into consideration analysis of the Ldace1 gene.

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“…The Colorado potato beetle's resistance to azinphosmethyl (an organophosphate) and carbofuran (a carbamate) were observed as early as the late 1970s [5]. This resistance is associated with a point mutation in AChE, a substitution of adenine with guanine at position 980 in the AChE gene, which leads to the change of serine to glycine (S291G) [86]. Furthermore, two other AChE mutations were identified in CPB, which were associated with resistance to these insecticides, R30K (replacement of the amino acid arginine with lysine) and I392T (replacement of isoleucine with threonine) [87].…”

Section: Insecticide Resistance-the Case Of Colorado Potato Beetlementioning

confidence: 99%

Difficulties in Potato Pest Control: The Case of Pyrethroids on Colorado Potato Beetle

Molnár

Rákosy-Tican

2021

Agronomy

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Colorado potato beetle (Leptinotarsa decemlineata, CPB) is considered one of the most persistent crop pests because it is highly adaptable and can rapidly develop insecticide resistance. Nowadays, this beetle is resistant to over 54 different insecticides. In the absence of competitive alternatives, the answer of farmers to the high degree of pest adaptability is to increase the number of pesticide treatments or apply chemicals with different modes of action. Such a strategy increases the risk of intoxication in non-target organisms and leads to environmental pollution, augmenting the carbon footprint. Furthermore, these strategies are also unsustainable and inefficient for pest management in the long-term. The time has thus come to reform existing agriculture practices, for which the implementation of an integrated pest management strategy would be a more feasible tool. Applying a sustainable pest management strategy is indispensable for a better understanding of the status and mechanisms of insecticide resistance. Effective pest management requires monitoring the resistance of pests and developing a well-programmed pesticide treatment to simultaneously reduce the insecticide selection pressure and environmental pollution. In this context, here we present a review on the difficulties of potato pest control using as a case study the resistance of CPB to pyrethroids.

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Detection of organophosphate and pyrethroid resistance alleles in Czech Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) populations by molecular methods

Cited by 21 publications

References 26 publications

Pre-invasion history and demography shape the genetic variation in the insecticide resistance-related acetylcholinesterase 2 gene in the invasive Colorado potato beetle

Pre-invasion history and demography shape the genetic variation in the insecticide resistance-related acetylcholinesterase 2 gene in the invasive Colorado potato beetle

Contribution of Ldace1 gene to acetylcholinesterase activity in Colorado potato beetle

Difficulties in Potato Pest Control: The Case of Pyrethroids on Colorado Potato Beetle

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