Detection of occult nasopharyngeal primary tumours by means of <i>in situ</i> hybridization

Feinmesser, Raphael; Feinmesser, Meora; Freeman, Jeremy L.; Noyek, Arnold M.; Livni, Nelly

doi:10.1017/s0022215100119450

Cited by 14 publications

(7 citation statements)

References 6 publications

(6 reference statements)

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“…amount of Grb7 bound to the Triton X-100-SMs to 6479 (n ¼ 5) in the absence of HRGb1, and to 56710 (n ¼ 5) in its presence. KN93 also increases the phosphorylation of ErbB2 (not shown), suggesting that CaMK-II downregulates this receptor as reported (Feinmesser et al, 1996).…”

Section: Grb7 Binds Calmodulin H LI Et Alsupporting

confidence: 70%

The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7

Sánchez-Torres

Carpio

et al. 2005

Oncogene

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We demonstrate using Ca 2 þ -dependent calmodulin (CaM)-affinity chromatography and overlay with biotinylated CaM that the adaptor proteins growth factor receptor bound (Grb)7 and Grb7V (a naturally occurring variant lacking the Src homology 2 (SH2) domain) are CaM-binding proteins. Deletion of an amphiphilic basic amino-acid sequence (residues 243-256) predicted to form an a-helix located in the proximal region of its pleckstrin homology (PH) domain demonstrates the location of the CaM-binding domain. This site is identical in human and rodents Grb7, and shares great homology with similar regions of Grb10 and Grb14, and the Mig10 protein from Caenorhabditis elegans. We show that Grb7 and Grb7V are present in the cytosol and bound to membranes, while the deletion mutants (Grb7D and Grb7VD) have less capacity to be associated to membranes. Grb7D maintains in part the capacity to bind phosphoinositides, and CaM competes for phosphoinositide binding. Activation of ErbB2 by heregulin b1 decreases the pool of Grb7 associated to membranes. The cell-permeable CaM antagonist W7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide), but not the CaM-dependent protein kinase II inhibitor KN93, prevents this effect. Highly specific cell-permeable CaM inhibitory peptides decrease the association of Grb7 to membranes. This suggests that CaM regulates the intracellular mobilization of Grb7 in living cells. Direct interaction between enhanced yellow fluorescent protein (EYFP)-Grb7 and enhanced cyan fluorescent protein (ECFP)-CaM chimeras at the plasma membrane of living cells was demonstrated by fluorescence resonance energy transfer (FRET). The FRET signal dramatically decreased in cells loaded with a cellpermeable Ca 2 þ chelator, and was significantly attenuated when enhanced yellow fluorescent protein-Grb7 chimera (EYFP-Grb7)D instead of EYFP-Grb7 was used. Finally, we show that conditioned media from cells transiently transfected with Grb7D and Grb7VD lost its angiogenic activity, in contrast to those from cells transiently transfected with their wild-type counterparts.

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Section: Grb7 Binds Calmodulin H LI Et Alsupporting

confidence: 70%

The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7

Sánchez-Torres

Carpio

et al. 2005

Oncogene

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“…Large reported series indicated that the frequency is around 3% of the total head and neck cancer cases 1–4. With the technologic advances in imaging5–7 and pathology,8–10 its incidence is expected to decrease further. Because of the rarity of this disease, all reports on treatment results of patients with SCC of unknown primary tumor site presenting with cervical neck nodes are retrospective studies.…”

Section: Discussionmentioning

confidence: 99%

Cervical nodal metastases from occult primary: Undifferentiated carcinoma versus squamous cell carcinoma

et al. 2002

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UDC histologic findings in our series are associated with better locoregional control and DSS than SCC. Our results in local control, emergence of primary tumor, and DSS are comparable with other published data. However, disease control of advanced nodal stage remains poor; more aggressive treatment approaches, like the use of concurrent chemoradiation or altered fractionation scheme, should be explored.

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“…Although EBV is carried by nearly all human adults, B Iymphocytes constitute the sole EBV reservoir, while persistent infection of epithelial cells is characteristic only of malignant tumors such as NPC 13 . Therefore, the detection of genomic DNA of EBV in nasopharyngeal biopsy specimens or neck metastases may be highly predictive of NPC 14 , 15 . Numerous studies performed outside of Southeast Asia have evaluated the role of EBV DNA detection in neck nodes using PCR and in situ hybridization and have demonstrated high sensitivity (89%‐100%) and specificity (78%‐100%) 14–18 .…”

mentioning

confidence: 99%

Epstein‐Barr virus DNA detection in the diagnosis of nasopharyngeal carcinoma

Yap

Hassan

Chan

et al. 2007

Otolaryngol.--head neck surg.

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Detection of occult nasopharyngeal primary tumours by means of in situ hybridization

Cited by 14 publications

References 6 publications

The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7

The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7

Cervical nodal metastases from occult primary: Undifferentiated carcinoma versus squamous cell carcinoma

Epstein‐Barr virus DNA detection in the diagnosis of nasopharyngeal carcinoma

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