Detection of new human papillomavirus sequences in skin lesions of a renal transplant recipient and characterization of one complete genome related to epidermodysplasia verruciformis-associated types.

Abstract

Human papillomavirus (HPV) DNA, originally isolated from patients suffering from the skin disease epidermodysplasia verruciformis (EV), and a growing number of related sequences have recently been detected in a high percentage of benign and malignant skin lesions of both immunosuppressed and immunocompetent people. HPV L1 DNA fragments (374-389 bp long) from a solar keratosis and a squamous cell carcinoma (SCC) of a renal transplant recipient were amplified, cloned and sequenced. In 54 clones, six different HPV sequences were identified. One of these six corresponded to the known type HPV-8 and two (RTRX3 and RTRX7) have been described previously in cutaneous lesions of immunosuppressed patients. The remaining three sequences were different from all known HPV types : an HPV-9-related sequence (77n4 % identity), an RTRX2-related sequence

Four novel human betapapillomaviruses of species 2 preferentially found in actinic keratosis

“…The viral loads in the HPV-positive patients were low, ranging from one viral copy per y35 000 cells to three copies per cell, a trend also observed by other groups (Bens et al, 1998;Meyer et al, 2001;Weissenborn et al, 2005). We found that SK lesions harboured higher amounts of virus than AK; however, due to the small number of observations, this finding requires further investigation.…”

Multiple bovine papillomavirus infections associated with cutaneous papillomatosis in brazilian cattle herds

“…This strategy has also allowed the identification of multiple infections and coinfection by distinct PV types in the same host (Forslund et al, 1999;Antonsson and Hansson, 2002;Ogawa et al, 2004;Maeda et al, 2007). Investigations in human beings have pointed to the occurrence of HPV mixed infection in different tumors collected from the same patient (Astori et al, 1997;Bens et al, 1998;Kay et al, 2002). On the other hand, studies with the aim of identifying BPV types associated with different skin warts from individual animals have not been performed.…”

Prevalence and type spectrum of human papillomaviruses in healthy skin samples collected in three continents

“…HPV-5, -8, -9, -12, -14, -15, -17 and -19-25 (Kremsdorf et al, 1984;Orth et al, 1978), has been found in these patients. Some skin HPV types, mainly HPV-5 and HPV-8, have been found in skin cancer lesions, for example in EV patients (Jablonska & Majewski, 1994, Orth et al, 1979 and in immunosuppressed patients (Bens et al, 1998;Berkhout et al, 2000;de Villiers et al, 1997), and have therefore been suggested to be high-risk HPVs for these patients. However, in recent years it has been found that skin HPV types are widely spread among humans, giving rise to subclinical infections generally without causing warts or other lesions (Antonsson et al, 2000;Astori et al, 1998;Boxman et al, 1997).…”