Detection of New Delhi Metallo-Beta-Lactamase (Encoded byblaNDM-1) inEnterobacter aerogenesin China

Shen, Yong; Wei, Xiao X.; Gong, Jiao-Mei; Pan, Jun; Xu, Qi

doi:10.1002/jcla.22044

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…The most common and unequivocal carbapenem resistance mechanism of Enterobacterales involves the production of carbapenemases and this study indicated that NDM-5 was the common carbapenemase in CRKA strains, which was different from other reports conducted in other countries ( 3 , 11 ). Notably, the detection rate for NDM type carrying Enterobacterales has risen in recent years.…”

Section: Discussioncontrasting

confidence: 99%

Emergence of NDM-5 Producing Carbapenem-Resistant Klebsiella aerogenes in a Pediatric Hospital in Shanghai, China

Pan

Zhang

2021

Front. Public Health

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Background: Carbapenem-resistant Klebsiella aerogenes (CRKA) has posed a serious threat for clinical anti-infective therapy. However, the molecular characteristics of CRKA in Shanghai are rarely reported.Objective: This study aimed to investigate the resistance profiles, dissemination mechanism, and molecular characteristics of CRKA strains isolated from children in a pediatric hospital, Shanghai.Method: Fifty CRKA isolates were collected in 2019. Antimicrobial susceptibility of the strains was determined by broth microdilution method. The β-lactamases and outer membrane porin genes were characterized by polymerase chain reaction (PCR). Conjugation experiments were performed to determine the transferability of the plasmids. The plasmids were typed based on their incompatibility group using the PCR-based replicon typing method. Multilocus sequence typing (MLST) and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) were performed for the genetic relationship.Results: All CRKA strains showed high level of resistance to cephalosporins and carbapenems, but still susceptible to aminoglycosides, colistin, and tigecycline. Forty five of fifty isolates carried blaNDM−5 genes (45/50, 90%), alongside with other β-Lactamase genes including blaCTX−M−1, blaTEM−1, and blaSHV−11 being detected. Loss of ompK35 and ompK36 genes were observed in 14% (7/50) and 28% (14/50), respectively, with 5 isolates lacking both ompK35 and ompK36. MLST analysis demonstrated that the majority of isolates belonged to ST4 (47/50, 94%) and ERIC-PCR fingerprinting was performed to identify NDM-5-producing isolates with approximately or more than 80% similarity levels. Plasmids carrying blaNDM−5 were successfully transferred to the E. coli recipient and plasmid typing showed that IncX3 were the prevalent among CRKA isolates.Conclusions: Our finding revealed the emergence of NDM-5 producing CRKA belonging to ST4 among children in Shanghai. Further attention should be paid to control the horizontal spread of the Class B carbapenemases like NDM in children.

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Section: Discussioncontrasting

confidence: 99%

Emergence of NDM-5 Producing Carbapenem-Resistant Klebsiella aerogenes in a Pediatric Hospital in Shanghai, China

Pan

Zhang

2021

Front. Public Health

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“…A nationwide surveillance study of clinical CRE isolates in China from June 2014 to June 2015 indicated that 1.2 to 18.9% of K. pneumoniae isolates were resistant to carbapenems (20). In the current study, the overall prevalence of CRKP (36.2%) among the K. pneumoniae isolates and the prevalence of CRE (18.1%) among the Enterobacteriaceae isolates between 2014 and 2016 were much higher than those recorded by previous reports from the same area (8,(20)(21)(22). Previous studies have indicated that CRE infections are associated with poor treatment outcomes (23).…”

Section: Discussioncontrasting

confidence: 70%

Rapid Increase in Prevalence of Carbapenem-Resistant Enterobacteriaceae (CRE) and Emergence of Colistin Resistance Gene mcr-1 in CRE in a Hospital in Henan, China

Sun

Shen

et al. 2018

J Clin Microbiol

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The global spread of carbapenem-resistant (CRE) is one of the most severe threats to human health in a clinical setting. The recent emergence of plasmid-mediated colistin resistance gene among CRE strains greatly compromises the use of colistin as a last resort for the treatment of infections caused by CRE. This study aimed to understand the current epidemiological trends and characteristics of CRE from a large hospital in Henan, the most populous province in China. From 2014 to 2016, a total of 7,249 isolates were collected from clinical samples, among which 18.1% (1,311/7,249) were carbapenem resistant. Carbapenem-resistant and carbapenem-resistant were the two most common CRE species, with carbapenemases (KPC) and New Delhi metallo-β-lactamases (NDM), respectively, responsible for the carbapenem resistance of the two species. Notably, >57.0% ( = 589) of the isolates from the intensive care unit were carbapenem resistant. Furthermore, and were found to coexist in one isolate, which exhibited resistance to almost all tested antibiotics. Overall, we observed a significant increase in the prevalence of CRE isolates during the study period and suggest that carbapenems may no longer be considered to be an effective treatment for infections caused by in the studied hospital.

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“…It can be used in both human and veterinary medicine; therefore, a large amount of antibiotics have been released into the environment. The blaNDM-1 gene is resistant to metallo-ß-lactamase and can degrade most antibiotics, including cephalosporins, penicillins, carbapenems and other ß-lactam antibiotics, leading to widespread antibiotic resistance [32]. The blaTEM gene is located on a mobile binding plasmid and transposon, enabling it to be transferred between different species.…”

Section: Absolute Abundance Of Argsmentioning

confidence: 99%

Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea

Su¹,

Li²,

Yang

et al. 2020

IJERPH

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Currently, due to abuse in the use of human antibiotics and the weak regulatory control that the authorities have over sewage discharge and manure management, antibiotic resistance genes (ARGs) have become a new type of environmental pollutant. Three different natural water bodies (Poyang Lake, Haihe River and Qingdao No.1 Bathing Beach seawater) were sampled during the same periods to conduct a longitudinal comparison of distribution. The distribution and expression of 11 ARGs in 20 species were studied, and the correlations between the expression and the distribution of time and space of the ARGs in different water bodies were also analyzed. With the exception of ermA, blaNDM-1 and vanA, which were not detected in seawater, the other ARGs could be detected in all three water bodies. Tetracycline resistance genes (tetC, tetM and tetQ) in the seawater and Haihe River had even reached 100%, and sulfa ARGs (sul1 and sul2) in the seawater and Poyang Lake, as well as sul2 and sul3 in the Haihe River, had also reached 100%. The ARG pollution in Haihe River was much more serious, since 14 and 17 of 20 ARG species were significantly higher compared with seawater and Poyang Lake, respectively. Some ARGs also had a high absolute abundance. The absolute abundance of macrolide resistance genes (ermB) in seawater was as high as 8.61 × 107 copies/L, and the anti-tuberculosis resistant genes (rpoB and katG) in the Haihe River Basin were highly abundant at 1.32 × 106 copies/L and 1.06 × 107 copies/L, respectively. This indicates that ARGs have gradually become more diverse and extensive in natural water bodies. The results of a redundancy analysis (RDA) of the three water bodies showed that although each water body is affected by different factors in space and time, overall, the presence of AGRs is closely related to the production and life of human beings and the migration of animals.

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Detection of New Delhi Metallo-Beta-Lactamase (Encoded bybla_NDM_-1) inEnterobacter aerogenesin China

Abstract: It is important to mandate prudent usage of antibiotics and implement infection control measures to control the spread of these resistant bla -positive strains.

Cited by 11 publications

References 14 publications

Emergence of NDM-5 Producing Carbapenem-Resistant Klebsiella aerogenes in a Pediatric Hospital in Shanghai, China

Emergence of NDM-5 Producing Carbapenem-Resistant Klebsiella aerogenes in a Pediatric Hospital in Shanghai, China

Rapid Increase in Prevalence of Carbapenem-Resistant Enterobacteriaceae (CRE) and Emergence of Colistin Resistance Gene mcr-1 in CRE in a Hospital in Henan, China

Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea

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