Detection of Mycoplasma pneumoniae by two polymerase chain reactions and role of Mycoplasma pneumoniae in pediatric community–acquired lower respiratory tract infections

Kumar, Surinder; Bharti, Pradeep; Baveja, C.P.; Mantan, Mukta; Saigal, S; Garg, Indu Bala

doi:10.1016/j.ijmmb.2022.01.002

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…9 Macrolides are widely used first-line medications for the treatment of M. pneumoniae, which is responsible for 40% of CAP worldwide and 30% of CAP in India. [10][11][12] Our investigation revealed that M. pneumoniae infection primarily occurs in children aged between 1-5 years, which was consistent with the previous reports. [13][14][15] Previous studies showed a significant difference in gender-based approaches, were girls being more susceptible to M. pneumoniae infection than boys.…”

Section: Discussionsupporting

confidence: 91%

“…18,19 Macrolides are widely used as firstline medications for M. pneumoniae infection which accounts for 40% of CAP worldwide and 30% of CAP in India. [10][11][12] Although tetracyclines, fluoroquinolones and erythromycin are effective in treating M. pneumoniae infection, they are not recommended for use in children due to their toxicity. Until the rise of the drug resistance in 2000, macrolides were the potential antibiotic in treating pneumonia caused by M. pneumoniae.…”

Section: Discussionmentioning

confidence: 99%

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Detection of Macrolide Resistant Mycoplasma pneumoniae in Children with Lower Respiratory Tract Infection by Sanger Sequencing Targeting Domain V Region of 23S rRNA Gene

Perumal¹,

Heamchandsaravanan²,

Shanmugam³

et al. 2023

J. Pure Appl. Microbiol.

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Mycoplasma pneumoniae is recognized as the leading cause of community-acquired lower respiratory tract infection in children, accounting for a significant proportion of pediatric mortality. Macrolides are the first-line treatment for M. pneumoniae infections. However, the extensive use of macrolides in clinical practice resulted in the emergence of macrolide-resistant M. pneumoniae (MRMP), which has a negative impact on treatment outcomes. Hence, in the present study, MRMP was determined in hospitalized children with community-acquired pneumonia (CAP). Real-time PCR detected M. pneumoniae in 41 of 348 clinical samples. Sanger sequencing revealed that none of the isolates were associated with the A2063G or A2064G base mutation, which confers macrolide resistance, in domain V of the 23S rRNA gene. Although MRMP was not observed in children with CAP in our study, healthcare practitioners should be vigilant about the potential risk of MRMP infections.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Detection of Macrolide Resistant Mycoplasma pneumoniae in Children with Lower Respiratory Tract Infection by Sanger Sequencing Targeting Domain V Region of 23S rRNA Gene

Perumal¹,

Heamchandsaravanan²,

Shanmugam³

et al. 2023

J. Pure Appl. Microbiol.

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“…[2,8,[16][17][18] For the child population, most studies have reported a higher positive rate for children aged > 5 years than for those < 5 years old [1,2,[18][19][20] ; the same result was observed in our study, which showed that children under the age of 6 years had the highest infection rate, which is thought to be related to incomplete immune mechanisms in this group and was easily infected by M pneumoniae. [5,21] Some studies have shown sex differences in M pneumoniae infections, [1,5,22,23] with some showing no differences. [16,18] In this study, no difference was observed; although the positive rates of females were higher than those of males in all 3 methods, the difference was not statistically significant.…”

Section: Discussionmentioning

confidence: 99%

Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children

Meng

Jiang

et al. 2023

Medicine

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This study aimed to evaluate the diagnostic value of chemiluminescence immunoassay (CLIA), passive particle agglutination (PPA), and indirect immunofluorescence assay (IFA) in detecting Mycoplasma pneumoniae infection in children. Serum samples from 165 children with acute community-acquired respiratory tract infections were examined using CLIA, PPA, and IFA, and consistency coefficient, specificity, and sensitivity were analyzed. Compared with the PPA (titer ≥ 1:160), the consistency coefficients of the immunoglobulin(Ig)M-CLIA, immunoglobulin(Ig)G-CLIA and IgM-IFA methods were 93.94%, 75.76%, and 83.64%, respectively. The positive likelihood ratio (PLR) and specificity of IgM-CLIA was 19.40 and 95.49%, respectively. The consistency coefficient of (IgM+IgG)-CLIA and PPA (titer ≥ 1:160) was 89.1%, and the sensitivity and negative predictive value of (IgM+IgG)-CLIA were 96.88% and 98.94%, respectively. CLIA MP-IgM has high concordance with PPA, and its specificity and sensitivity are higher than those of CLIA MP-IgG and IFA MP-IgM, suggesting its better diagnosis of early MP infection. The sensitivity and negative predictive value of CLIA MP (IgM+IgG) were higher than those of PPA or IFA, indicating that it should be considered as a priority in the diagnosis of MP infection.

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Increased Carotid Intima-media Thickness and Its Association with Carbohydrate Metabolism and Adipocytokines in Children Treated with Recombinant Growth Hormone

Saygılı¹,

Kocaaga²,

Kaya³

et al. 2023

Jcrpe

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Objective: Reports on the association between growth hormone (GH) therapy and cardiovascular risk factors in children are limited. This study aimed to evaluate carotid intima-media thickness (cIMT) in children treated with recombinant human GH (rhGH) and assess the effects of rhGH therapy and changes in serum carbohydrate metabolism, lipid profile and adipocytokines on cIMT. Methods: Seventy-one isolated idiopathic GH deficiency (GHD) children and 44 age- and sex-matched healthy controls were enrolled in this study. The study group was divided into two subgroups according to insulin resistance (IR) on oral glucose tolerance tests. Insulin secretion [homeostatic model assessment (HOMA) B, total insulin] and sensitivity (HOMA-IR, QUICKI, Matsuda) indices were calculated. cIMT was measured and the standard deviation scores (SDS) were calculated. Associations between cIMT-SDS and insulin secretion and sensitivity indices, serum lipid levels, adipocytokines (leptin, resistin, ghrelin), and other rhGH treatment-related factors were evaluated. Results: cIMT-SDS was increased in GHD children treated with rhGH compared to the controls [0.02 (2.27) vs. -1.01 (1.63), p=0.003]. cIMT-SDS did not differ between those children on rhGH treatment with or without IR. High cIMT-SDS was significantly associated with higher serum ghrelin levels and lower serum high density lipoprotein (HDL) levels (β=0.491, p=0.001 and β=-0.027, p=0.017), but not with BMI-SDS, blood pressure, insulin secretion and sensitivity indices, or the dose and duration of rhGH therapy. Conclusion: Our findings showed that GHD children treated with rhGH have increased cIMT. Alterations in carbohydrate metabolism were not associated with cIMT in children treated with rhGH. GH therapy per se appears to be associated with this increased cIMT but causality should be elucidated in further studies. cIMT also appears to be associated with higher ghrelin and lower HDL levels.

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Detection of Mycoplasma pneumoniae by two polymerase chain reactions and role of Mycoplasma pneumoniae in pediatric community–acquired lower respiratory tract infections

Cited by 3 publications

References 14 publications

Detection of Macrolide Resistant Mycoplasma pneumoniae in Children with Lower Respiratory Tract Infection by Sanger Sequencing Targeting Domain V Region of 23S rRNA Gene

Detection of Macrolide Resistant Mycoplasma pneumoniae in Children with Lower Respiratory Tract Infection by Sanger Sequencing Targeting Domain V Region of 23S rRNA Gene

Comparison of serodiagnosis methods for community-acquired Mycoplasma pneumoniae respiratory tract infections in children

Increased Carotid Intima-media Thickness and Its Association with Carbohydrate Metabolism and Adipocytokines in Children Treated with Recombinant Growth Hormone

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