Detection of mutant p53 using field-effect transistor biosensor

Surface Plasmon Resonance: A Versatile Technique for Biosensor Applications

“…Only wild type DNA showed signal boosting by PCR, while mutant DNA showed no SPR signal amplification. Mutation in protein molecules has also been studied using the SPR technique [ 59 ]. The DNA-binding capability of tumor protein p53 was evaluated.…”

A sandwich type immunosensor for ultrasensitive electrochemical quantification of p53 protein based on gold nanoparticles/graphene oxide

“…In order to meet the increasing demand for detection of p53, a variety of methods have been established and used for detection of this important protein including high-performance liquid chromatography [7], surface plasmon resonance [8], surface-enhanced Raman spectroscopy [9], field-effect transistor [10], matrixassisted laser desorption/ionization time-of-flight mass spectrometry [11], immunohistochemical method [12], and enzyme-linked immunosorbent assay [13]. All of these methods have their own advantages, but also have some drawbacks such as timeconsuming, inflexibility, lower sensitivity, exuberant instruments and a need for highly skilled personnel.…”

Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)—An Electrochemical Optimisation Approach

“…The system compared both wildtype and mutant TP53 DNA, and after binding, a significant increase in FET drain current was noted with the wildtype sequence (~250 µA), whereas for the mutant sequence, the drain current only increased by~20 µA for the same 100 nM target DNA concentration, confirming specificity of the system. In [36], the authors also provide validation for our increase in FET potential, since the TP53 protein which is positively charged shields the consensus DNA on the gate surface, and an increase in positive charge on the gate surface will increase the FET channel conductance, and thus the drain current, and in our case, potential, will increase. Whilst highly specific to TP53 DNA, the main drawback of these systems is their fabrication complexity as well as relatively high cost for development.…”