A sandwich type immunosensor for ultrasensitive electrochemical quantification of p53 protein based on gold nanoparticles/graphene oxide

Afsharan, Hadi; Khalilzadeh, Balal; Tajalli, H.; Mollabashi, Mahmoud; Navaeipour, Farzaneh; Rashidi, Mohammad-Reza

doi:10.1016/j.electacta.2015.11.133

Cited by 64 publications

(18 citation statements)

References 49 publications

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“…Moreover, the use of GO and Strept-AuNPs not only improved the electron transfer rate, but also increased the amount of anti-p53 capture antibody. The proposed immunosensor was validated by detection of p53 protein in spiked human serum samples and also in normal and cancerous human skin fibroblast cells [83]. …”

Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

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Early diagnosis is often the key to successful patient treatment and survival. The identification of various disease signaling biomarkers which reliably reflect normal and disease states in humans in biological fluids explain the burgeoning research field in developing new methodologies able to determine the target biomarkers in complex biological samples with the required sensitivity and selectivity and in a simple and rapid way. The unique advantages offered by electrochemical sensors together with the availability of high affinity and specific bioreceptors and their great capabilities in terms of sensitivity and stability imparted by nanostructuring the electrode surface with different carbon nanomaterials have led to the development of new electrochemical biosensing strategies that have flourished as interesting alternatives to conventional methodologies for clinical diagnostics. This paper briefly reviews the advantages of using carbon nanostructures and their hybrid nanocomposites as electrode modifiers to construct efficient electrochemical sensing platforms for diagnosis. The review provides an updated overview of some selected examples involving attractive amplification and biosensing approaches which have been applied to the determination of relevant genetic and protein diagnostics biomarkers.

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Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

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“…Tumor biomarkers are substances or processes associated with cancer whose levels in biological fluids or body tissues can provide essential information for clinical cancer screening and early cancer detection [4]. To reduce and control cancer, implementation of evidence-based strategies for its prevention, early detection and management of patients is needed.…”

Section: Introductionmentioning

confidence: 99%

“…It is considered to play a crucial role in the regulation of the cell cycle, DNA repair, and programmed cell death, inhibiting the growth of tumor cells through eliciting either cell-cycle arrest or apoptosis. In cells with disruption in cell proliferation, p53 is activated and bound to the specific DNA sequences and, as a result, the uncontrolled cell growth is stopped or the damaged DNA is eliminated [4]. Loss of p53 function results in induction of tumors and gene mutation, which is caused by the conformational changes in the p53 protein structure [3,4,7,8].…”

Section: Introductionmentioning

confidence: 99%

“…In cells with disruption in cell proliferation, p53 is activated and bound to the specific DNA sequences and, as a result, the uncontrolled cell growth is stopped or the damaged DNA is eliminated [4]. Loss of p53 function results in induction of tumors and gene mutation, which is caused by the conformational changes in the p53 protein structure [3,4,7,8]. Mutations of the p53 gene are the most common genetic alterations in human cancers.…”

Section: Introductionmentioning

confidence: 99%

“…Magnetic particles (MBs) were used to immobilize the capture antibodies and the lead ions liberated from the Pb 3 (PO 4 ) 2 core within the apoferritin shell were detected by stripping SWV at an in situ plated Bi film formed on a SPCE. Very recently, Afsharan et al [4] have proposed the detection of p53 protein sandwiched between a biotinylated capture antibody immobilized on the surface of a GCE using thiolated graphene oxide/streptavidin-AuNPs and a secondary antibody labeled with HRP. The electrocatalytic reduction of thionine in the presence of hydrogen peroxide was, again, used in the monitoring of the immunocomplex formation.…”

Section: Introductionmentioning

confidence: 99%

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Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers

et al. 2016

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An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation.

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Strategic Applications of Nanomaterials as Sensing Platforms and Signal Amplification Markers at Electrochemical Immunosensors

Huo

Liu

et al. 2016

Electroanalysis

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Tremendous developments in electrochemical immunosensors have facilitated cost‐effective, rapid and robust detection of many analytes. Among the significant developments, electrochemical immunosensors capable of simultaneously detecting several analytes have begun to emerge. In addition, recent progress in materials science and engineering has yielded nanostructured materials of various geometries that were readily exploited to provide a compatible medium for many biomolecules in immunoassays. Applications of these nanostructured materials to electrochemical immunosensor developments have significantly improved sensor performance in terms of their electrical, chemical and transport properties. This review provides a survey of innovative immunosensor developments involving a range of nanomaterials exploited in designing sensing platforms and efficient detection markers to achieve substantial signal amplifications and thereby outstanding detection characteristics.

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A sandwich type immunosensor for ultrasensitive electrochemical quantification of p53 protein based on gold nanoparticles/graphene oxide

Cited by 64 publications

References 49 publications

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers

Strategic Applications of Nanomaterials as Sensing Platforms and Signal Amplification Markers at Electrochemical Immunosensors

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