Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases

Introduction: The aim of the study was to optimise and compare two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples including the liver, brain, and blood. Material and Methods: Three strains of L. monocytogenes and single strains of each of the species:welshimeri, and L. seeligeri were used. Additionally, five other species of bacterium were used to evaluate the specificity of the tests. Results: Specific amplification products were obtained for both multiplex PCR assays, which confirmed the tested strains as Listeria spp. and L. monocytogenes, respectively. Isolates of other species did not yield PCR products. Conclusion: Both multiplex PCR assays proved to be significantly sensitive and highly-specific methods for the detection of Listeria strains.

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“…Several researchers used different concentrations of primers depending on the reaction mixtures applied and conditions of PCR (1,2,4,6,22,23).…”

Section: Discussionmentioning

confidence: 99%

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Budniak

Kędrak-Jabłońska

Szczawińska

et al. 2016

Journal of Veterinary Research

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“…Also detection of hly and plcA gene by PCR in L. monocytogenes isolates is not sufficient to elucidate the true pathogenic potential, because both the gene are regulated by a key regulator gene i.e., prfA (Shakuntala et al, 2006;Kaur et al, 2007;Rawool et al, 2007b;Aurora et al, 2008). In addition, other genes such as actA, internalins and several other virulent associated genes do play an essential role in pathogenesis of this bug.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of pathogenic Listeria monocytogenes from diarrhoeal cases in human infants and young animals

Negi¹

2014

Adv. Anim. Vet. Sci.

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Studies addressing isolation of Listeria monocytogenes from febrile gastroenteritis cases are very sparse. In the present study sporadic febrile diarrheal cases in human infants (less than 5 years of age) and young animals (0 -6 months) were initially analyzed for isolation of L. monocytogenes. The isolates recovered were further characterized for their virulence potential using in vitro and in vivo pathogenicity tests and serogrouping by multiplex serotyping PCR. A total of 315 diarrhoeal samples (165 human infants and 150 young animals) were collected and analyzed for isolation of L. monocytogenes. On microbiological and biochemical analysis, four L. monocytogenes isolates were identified, two each from human infants and piglet respectively. The overall isolation rate of L. monocytogenes was very low in human infants (1.2%) and piglets (1.3%); however all the isolates were found to be highly pathogenic when assessed using in vitro and in vivo assays. Further, serotyping revealed that all the four L. monocytogenes isolates, belonged to serogroup 4b, 4d, 4e, which is a matter of great concern as serovar 4b in particular, is generally associated with listerial foodborne outbreaks worldwide. To conclude, isolation of pathogenic L. monocytogenes from sporadic febrile diarrheal cases (human infants and piglets) appears to be the first report in India. Thus, from future human and animal health perspective, due consideration should be imposed for either isolation or detection of L. monocytogenes from febrile gastroenteritis cases. All copyrights reserved to Nexus® academic publishers

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“…Reliable diagnosis of listeriosis is made by cultural methods, however, the isolation as well as the differentiation of pathogenic and non-pathogenic Listeria remains a time consuming and tedious task [13]. The pathogenic potential of Listeria isolates can be assessed by PCR multiple key virulence factors such as hlyA [14], internalin A (inlA), internalin B (inlB) [15], actA, plcA [16], plcB [17].…”

Section: Introductionmentioning

confidence: 99%

Detection of <i>Listeria monocytogenes</i> in Foods and Characterization by PFGE

Park¹,

Jung²,

Lee³

et al. 2016

AiM

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The aims of the present study were to investigate the prevalence of Listeria monocytogenes in 1042 foods collected from different market to characterize the isolates by phenotypical and molecular methods. In particular, L. monocytogenes obtained from different types of foods such as RTE (kimbap), fish (smoked salmon and seasoned-dried slice fish) and meat (cut raw beef and pork) from 2009 to 2011, were used. Twelve samples (2.1%) were positive for L. monocytogenes. Detection rate of L. monocytogenes varied significantly by food type and ranged from 1.1% to 5.2%. Meat is the highest prevalence for L. monocytogenes (5.2%) followed by RTE (1.8%) and Fish (1.1%). Twelve isolates were also serotyped by the agglutination method. The most common serotypes detected in the 12 strains tested were 1/4b (75.0%), followed by 1/2a (16.7%), and 1/2b (8.3%). For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. PFGE was performed to determine genetic characterization of L. monocytogenes strains to define the genetic diversity.

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Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases

Cited by 86 publications

References 19 publications

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Isolation and Identification of pathogenic Listeria monocytogenes from diarrhoeal cases in human infants and young animals

Detection of <i>Listeria monocytogenes</i> in Foods and Characterization by PFGE

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Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases

Cited by 86 publications

References 19 publications

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Isolation and Identification of pathogenic Listeria monocytogenes from diarrhoeal cases in human infants and young animals

Detection of &lt;i&gt;Listeria monocytogenes&lt;/i&gt; in Foods and Characterization by PFGE

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Detection of <i>Listeria monocytogenes</i> in Foods and Characterization by PFGE