Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Budniak, S.; Kędrak-Jabłońska, Agnieszka; Szczawińska, Anna; Reksa, M.; Krupa, Marek; Szulowski, Krzysztof

doi:10.1515/jvetres-2016-0061

Cited by 5 publications

(2 citation statements)

References 28 publications

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“…All isolates showed the inlA gene (Fig. 1) which confirmed L. monocytogenes (Budniak et al, 2016). This finding supports previous studies that identified the presence of internalin genes in L. monocytogenes isolates from irrigation water and agricultural soil in South Africa's Eastern Cape Province (Iwu et al, 2022).…”

Section: Resultssupporting

confidence: 88%

Antimicrobial susceptibility patterns of Listeria monocytogenes isolated from fresh produce in KwaZulu-Natal Province, South Africa

F Tshabuse,

NK Cele,

AR Opoku

et al. 2024

WSA

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Fresh, ready-to-eat produce is frequently irrigated with untreated water, making it a leading cause of food-borne illness outbreaks worldwide. This study investigated the presence of Listeria monocytogenes in fresh produce that was grown using river water. Standard biochemical tests were used for the identification of L. monocytogenes isolated from river water used for irrigation, and from fresh produce including lettuce, spinach, and pumpkin. The inlA gene of L. monocytogenes was molecularly identified using PCR amplification. The susceptibility of L. monocytogenes isolates to antimicrobial agents was assessed using the Kirby-Bauer disc diffusion method. The presence of the amplified inlA gene (800 bp) indicated that all of the fresh produce and river water samples were contaminated with virulent L. monocytogenes. Lettuce and spinach exhibited higher quantities of L. monocytogenes, with lettuce recording 87 CFU/g and spinach recording 71 CFU/g. The L. monocytogenes isolates from spinach and lettuce sources showed significant resistance to colistin (56.2% and 53.3%, respectively) as well as ampicillin (68.8% and 53.3%, respectively). Moreover, lettuce (40%) and spinach (31%) exhibited a common resistance pattern of AMP-CHL-CT-KAN-PIP-ERY-TET, with a maximum MAR index value of 0.54. Our research demonstrates the transmission of multidrug-resistant L. monocytogenes from irrigation river water to fresh produce. Hence, the ingestion of ready-to-eat fresh produce carries the potential for human listeriosis, particularly among individuals with compromised immune systems.

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Section: Resultssupporting

confidence: 88%

Antimicrobial susceptibility patterns of Listeria monocytogenes isolated from fresh produce in KwaZulu-Natal Province, South Africa

F Tshabuse,

NK Cele,

AR Opoku

et al. 2024

WSA

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“…However, extracts made of 50% ethanol, which made them available for in vivo or in vitro, are more specific for animal probes and provide more scientific evidence from herbs as an alternative antibiotic or feed additive useful for horse nutrition [45]. These extracts could be proved more specific in further antimicrobial tests over keratoconjunctivitis etiological agents (L. monocytogenes) or other horse disease agents (S. aureus or Salmonella species) [16,40,41].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Two Medicinal Plants Cuphea aequipetala var. hispida (Cav.) Koehne and Eryngium comosum Delaroche F Against Bacteria Related to Equine Infections

Alvarado

Berasain

Salem

et al. 2020

Journal of Equine Veterinary Science

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Functional biocompounds beneficial for animals and humans are in Mexican folk herbs. Cuphea and Eryngium species presented antimicrobial potential. Natural antibiotic uses by ethnoveterinary research with medicinal plants in equine infection or digestive diseases need more scientific evidence. Staphylococcus aureus, Escherichia coli, Salmonella enterica serotype Enteritidis are etiological agents in horses responsible for stable infections, abortions, fetal or perinatal deaths, and resistant intrahospital infections. The main objective of the present research was to evaluate the potential of antimicrobial and antioxidant activities of two Mexican medicinal plants Cuphea aequipetala var. hispida (Cav.) Koehne and Eryngium comosum Delaroche F over Listeria monocytogenes ATCC 19115, Staphylococcus sp., E. coli ATCC 25922, and S. enterica serotype Enteritidis ATCC 13076 bacterium reference strains related to equine infections. Determination of total phenol, saponins, antioxidant activity (ABTS), and antimicrobial activity with diffusion-sensitive discs was performed in triplicate. All the strains were sensitive for both extracts except for E. coli strain that was inhibited only by C. aequipetala. Staphylococcus sp. and S. enterica strains were inhibited equally by both extracts. E. comosum extracts tested have shown the highest effect over L. monocytogenes. In summary, antimicrobial activity was similar to the reported activity of Eryngium species extracts with other different solvents. Present extracts are suggested as a potential alternative antibiotic; definitely, more specific equine pathogen inhibition tests are needed in feed additives for horse nutrition research. In conclusion, antimicrobial activities of Cuphea aequipetala var. hispida (Cav.) Koehne and Eryngium comosum Delaroche F over reference strains related to equine infections suggested these medicinal plants as potential antibiotic sources for horse diseases.

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Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification

Ledlod

Bunroddith

Areekit

et al. 2020

Journal of Chromatography B

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Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Cited by 5 publications

References 28 publications

Antimicrobial susceptibility patterns of Listeria monocytogenes isolated from fresh produce in KwaZulu-Natal Province, South Africa

Antimicrobial susceptibility patterns of Listeria monocytogenes isolated from fresh produce in KwaZulu-Natal Province, South Africa

Antimicrobial and Antioxidant Activities of Two Medicinal Plants Cuphea aequipetala var. hispida (Cav.) Koehne and Eryngium comosum Delaroche F Against Bacteria Related to Equine Infections

Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification

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