Detection of multiple circulating Leishmania species in Lutzomyia longipalpis in the city of Governador Valadares, southeastern Brazil

Cardoso, Mariana Santos; Bento, Gabrielle Ariadine; Almeida, Laila Viana de; Castro, Joseane Camilla de; Reis-Cunha, João Luís; Barbosa, Vanessa de Araújo; Souza, Cecília Pereira de; Brazil, Reginaldo Peçanha; Valdivia, Hugo O.; Bartholomeu, Daniella Castanheira

doi:10.1371/journal.pone.0211831

Cited by 14 publications

(7 citation statements)

References 43 publications

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“…Visceral leishmaniasis remains one of the most prevalent parasitic diseases, with outbreaks and potential mortality 4 . In South America, Brazil is one of most endemic regions for VL and CL 5 .…”

Section: Introductionmentioning

confidence: 99%

Parasitological and molecular diagnosis of cutaneous leishmaniasis among indigenous peoples in the state of Roraima, Brazil.

Almeida

Souza²,

Teixeira

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: We diagnose cases of cutaneous leishmaniasis (CL) among indigenous peoples of the state of Roraima, Brazil, and discuss some aspects of its epidemiology. Methods: Skin imprints, and lesion exudate samples collected on filter paper were examined using parasitological and molecular techniques, respectively. Results: Of 30 indigenous individuals, representing several ethnic groups, with suspected cases of CL, 27 (90%) tested positive for Leishmania spp. by PCR, and 21 (70%) by parasitological microscopy. Conclusions: Cutaneous leishmaniasis is indistinctly present among indigenous peoples from different regions of the state of Roraima. Individuals from seven of the ten existing ethnic groups in the state tested positive for CL, demonstrating the need for further investigation of the disease among these ethnic groups.

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Section: Introductionmentioning

confidence: 99%

Parasitological and molecular diagnosis of cutaneous leishmaniasis among indigenous peoples in the state of Roraima, Brazil.

Almeida

Souza²,

Teixeira

et al. 2020

Rev. Soc. Bras. Med. Trop.

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“…Experimental infection data have shown that infection by Leishmania amazonensis in Migonemyia migonei is possible [34,35]. It is important to mention that Leishmania amazonensis have been recorded in Minas Gerais in sand ies and dogs [31,[36][37][38]. Therefore, the present study suggests that Leishmania amazonensis may also be a possible agent of leishmaniasis in the municipality of Timóteo.…”

Section: Detection Of Leishmania Spp Dnamentioning

confidence: 66%

Detection Molecular of Leishmania Spp. And Blood Source in Female Sand Flies in the Parque Estadual Do Rio Doce and in the Municipality of Timóteo, Minas Gerais, Brazil.

Souza

Santos

Bevilacqua

et al. 2021

Preprint

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Background: Leishmaniasis is a set of diseases caused by protozoa of the Leishmania genus (Kinetoplastida: Trypanosomatidae) and transmitted by the bite of female sand fly insects (Diptera: Psychodidae). The present work was characterized as a descriptive study comparing two areas: a wild area where the Parque Estadual do Rio Doce (PERD) is located and another urban area, located in the municipality of Timóteo-MG (AUT). Aiming to identify the presence of Leishmania spp. and the blood source of collected female sand flies. Methods: Sand flies were collected with CDC light trap model HP, between September 2012 and February 2014. We used 19 traps in the Urban Area of Timóteo and 12 in the Rio Doce State Park. For molecular studies of Leishmania spp. DNA we used ITS1 gene and in the search of blood source, with naturally fed females, the CytB gene was used and positive samples were sequenced. Results: It was clear that in the municipality of Timóteo there are at least three species of Leishmania: Leishmania (Viannia) braziliensis, Leishmania (Leishmania) amazonensis and Leishmania (V.) guyanensis. A large presence of Leishmania braziliensis DNA was identified in female sand flies. Nyssomyia whitmani was the most captured species in the urban area, while in the PERD it was Pressatia choti, both species were positive for the presence of Leishmania braziliensis DNA. And last but not least was the record of the participation of Gallus gallus (chicken) and Sus scrofa (pig) as a blood source for sandflies, which helps to maintain the life cycle of vectors in the urban area of the municipality.Conclusion: The present study demonstrates that Leishmania braziliensis is the main tegumentary leishmaniasis transmitter agent in the study area, with effective participation of Nyssomyia whitmani as a vector. And it draws attention to the possibility that Gallus gallus (chicken) and Sus scrofa (pig) are fundamental in maintaining the cycle in urban areas.

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“…longipalpis infected with multiple Leishmania spp . have been also been found[58] indicating that the epidemiological features require further work.…”

Section: Discussionmentioning

confidence: 96%

eNose analysis of volatile chemicals from dogs naturally infected with Leishmania infantum in Brazil

Staniek

Sedda

Gibson³

et al. 2019

PLoS Negl Trop Dis

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Background Visceral leishmaniasis (VL) in Brazil is a neglected, vector-borne, tropical parasitic disease that is responsible for several thousand human deaths every year. The transmission route involves sand flies becoming infected after feeding on infected reservoir host, mainly dogs, and then transmitting the Leishmania infantum parasites while feeding on humans. A major component of the VL control effort is the identification and euthanasia of infected dogs to remove them as a source of infection. A rapid, non-invasive, point-of-care device able to differentiate between the odours of infected and uninfected dogs may contribute towards the accurate diagnosis of canine VL. Methodology/Principal findings We analysed the headspace volatile chemicals from the hair of two groups of dogs collected in 2017 and 2018 using a bench-top eNose volatile organic chemical analyser. The dogs were categorised as infected or uninfected by PCR analysis of blood samples taken by venepuncture and the number of parasites per ml of blood was calculated for each dog by qPCR analysis. We demonstrated using a robust clustering analysis that the eNose data could be discriminated into infected and uninfected categories with specificity >94% and sensitivity >97%. The eNose device and data analysis were sufficiently sensitive to be able to identify infected dogs even when the Leishmania population in the circulating blood was very low. Conclusions/Significance The study illustrates the potential of the eNose to rapidly and accurately identify dogs infected with Le . infantum . Future improvements to eNose analyser sensor sensitivity, sampling methodology and portability suggest that this approach could significantly improve the diagnosis of VL infected dogs in Brazil with additional potential for effective diagnosis of VL in humans as well as for the diagnosis of other parasitic diseases.

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Detection of multiple circulating Leishmania species in Lutzomyia longipalpis in the city of Governador Valadares, southeastern Brazil

Cited by 14 publications

References 43 publications

Parasitological and molecular diagnosis of cutaneous leishmaniasis among indigenous peoples in the state of Roraima, Brazil.

Parasitological and molecular diagnosis of cutaneous leishmaniasis among indigenous peoples in the state of Roraima, Brazil.

Detection Molecular of Leishmania Spp. And Blood Source in Female Sand Flies in the Parque Estadual Do Rio Doce and in the Municipality of Timóteo, Minas Gerais, Brazil.

eNose analysis of volatile chemicals from dogs naturally infected with Leishmania infantum in Brazil

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