Detection of Multidrug Resistance Genes in Pseudomonas aeruginosa Isolated from Bovine Mastitic Milk

Abstract

A total of 270 milk samples were collected from private and governmental cow`s farms (200 samples from mastitic cows and 70 from apparently healthy cows). The samples were collected from different provinces (130 samples from Dakahlia, 66 samples from Sharkia and 74 samples from Damieta). Twenty isolates of P. Aeruginosa were isolated from 270 total milk samples in a percentage of 7.4. Damietta providence showed high percentage P. Aeruginosa isolates 8.1% followed by Sharkia providence and Dakhlia providence (7.6% and 6.9% respectively). Biochemical identification provided 16 distinct biotypes. Isolates that used galactose, mannose, manitol, displayed a green pigment and β hemolysis belonged to the more frequent biotype (80%). Most isolates showed multidrug resistance to antibiotic disc used (17 types) the rate of resistance ranged from 25%-100%. In Quinolone group, 85% of isolates were resistant to nalidixic acid and in flouroquinolne group, the resistance was ofloxacin enrofloxacin, followed by ciprofloxacin, and (95%, 85% and 80% respectively). In aminoglycosides group, 70% of isolates were resistance to neomycin followed by amikacin (65%) and gentamycin (50%). In polypeptides and sulphonamides groups, all isolates were resistance to colstin sulfate and sulfa methaxozoletrimethoprim, while in β-Lactams pencillin, macrolide, tetracycline and chloramphenicol groups, 95% of isolates were resistance to ampicillin, Erythromycin, tetracycline and chloramphenicol repectively. In lincomides group, 85% of isolates were resistance to both lincomycin and Clindamycin. On the other hand, in β-lactams (Cephalosporine) group, the resistance was only 30% to cefadroxil and 25% to and cephalothin. The detection of β-lactams multidrug resistance genes using multiplex PCR in 20 isolates was observed where 10% of isolates (2 isolates) harbour LAT-1 to LAT4, CMY-2 to CMY-7, BIL-1 genes; 4 isolates (20%) harbour MIR-IT ATC-1 and all is dolates harbour DHA-1, DHA-3 genes. The Detection of flouroquinolone multidrug resistance genes in 20 P. Aeruginosa isolates using singplex PCR, where 6 isolates harbored ParC gene and all isolates harbored gyrA gene.

Assessment of Phytochemical, Antioxidant and Antibacterial Activity of Balanites Aegyptiaca and Curcuma Longa against Some Bacterial Pathogens Isolated from Dairy Cow Infected with Mastitis

“…Mastitis is considered the most common serious health problem on dairy farms which lead to significant bio health hazard to human particularly in developing countries such as in Egypt (Ammar et al, 2016). Clinical inspection and the California mastitis test were found to be effective diagnostic tools for detecting and distinguishing clinical and subclinical mastitis from apparently healthy cattle, which is consistent with previous findings (Gianneechini et al, 2002).…”