Detection of molecular markers linked to the durable adult plant stripe rust resistance gene Yr18 in bread wheat (Triticum aestivum L.)

Abstract

Stripe rust of wheat caused by Puccinia striiformis West. f. sp. tritici presents a serious problem for wheat production worldwide, and identification and deployment of resistance sources to it are key objectives for many wheat breeders. Here we report the detection of simple sequence repeat (SSR) markers linked to the durable adult plant resistance of cv. ÔOtaneÕ, which has conferred this resistance since its release in New Zealand in 1984. A double haploid population from a cross between ÔOtaneÕ and the susceptible cv. ÔTiriteaÕ was visually assessed for adult plant infection types (IT) in the glasshouse and field, and for final disease severity in the field against stripe rust pathotype 106E139A + . At least three resistance loci controlled adult plant resistance to stripe rust in this population. Quantitative trait loci (QTL) mapping results revealed that two of these, one on chromosome 7DS corresponds to the durable adult plant resistance gene Yr18 and other on chromosome 5DL were contributed from ÔOtaneÕ; while the remaining one on chromosome 7BL, was contributed from the susceptible ÔTiriteaÕ. Interval mapping placed the ÔOtaneÕ-resistant segment near the centromere of chromosome 7DS at a distance of 7 cM from the SSR marker gwm44. The stability of QTL in the two environments is discussed. SSR gwm44 is potentially a candidate marker for identifying the durable resistance gene Yr18 in breeding programmes.

More precise map position and origin of a durable non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) in wheat

“…In wheat linkage of stripe rust and powdery mildew resistances with molecular marker Xgwm583 was found on chromosome 5DL (Imtiaz et al 2004). Another recently identified slow rusting gene Yr29 (1BL) is completely linked to gene Lr46, which confers moderate resistance to leaf rust (William et al 2003).…”

Characterization of Quantitative Trait Loci Controlling Genetic Variation for Preharvest Sprouting in Synthetic Backcross-Derived Wheat Lines

“…Genomic DNA was extracted from the two parents and 271 BC 1 F 7 lines using the standard phenol/chloroform method as described by Imtiaz et al (2004). About 433 SSR markers, available in the public domain (http:/ /wheat.pw.…”

Molecular markers: actual and potential contributions to wheat genome characterization and breeding

“…SSR markers were associated with many QTL, e.g. those rendering resistance to leaf rust (Schnurbusch et al 2004), yellow rust (Imtiaz et al 2004 Zhou et al 2002Zhou et al , 2003Yang et al 2005), Altolerance Navakode et al 2006;Zhou et al 2007), seed dormancy (Kato et al 2001;Mares et al 2004;Lohwasser et al 2005), high grain protein content (Prasad et al 2003), and yield (Huang et al 2003b;Verma et al 2005).…”