More precise map position and origin of a durable non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) in wheat

Abstract

Recently a major gene determining non-specific adult plant disease resistance against stripe rust (Puccinia striiformis) designated Yrns-B1 was mapped in wheat Triticum aestivum L. by using a cross between 'Lgst. 79-74' (resistant) and 'Winzi' (susceptible). Linkage to five Gatersleben wheat microsatellite (GWM) markers was discovered, previously mapped on chromosome arm 3BS. In the present study this map was improved by the incorporation of four additional GWM markers. QTL-analysis revealed high LOD values for the resistance at all nine loci, whereas the largest LOD (20.76) was found for the newly mapped marker Xgwm1329.Microsatellite analysis and resistance tests of a collection of old German/UK wheat varieties, including probable ancestors of 'Lgst.79-74' were carried out. A high coincidence of non-specific adult plant disease resistance against stripe rust and the presence of 'Lgst. 79-74' allele (117 bp) of the marker Xgwm533 was observed among the varieties tested. Linkage during the inheritance of both the resistance and the 117 bp allele of Xgwm533 was demonstrated. The probable origin of Yrns-B1 is discussed. Carriers of this resistance gene were grown on large areas since more than 100 years. To estimate the capability of Xgwm533 as a diagnostic marker for non-specific adult plant disease resistance against stripe rust, microsatellite analysis and resistance tests of a collection of Russian spring wheat varieties were performed. The 117 bp allele of Xgwm533 was found in about 35% of the Russian cultivars analysed, however, none of them possessed the expected disease resistance. Thus, the utilisation of Xgwm533 as diagnostic marker seems to be restricted to certain genepools.

An EST-SSR marker linked with yellow rust resistance in wheat

“…An F 2 population from a cross between PI178383 and Harmankaya99 was visually assessed for seedling infection type in greenhouse. Similar to our work, Khlestkina et al (2007) identified Xgwm533 as a diagnostic marker for disease resistance against yellow rust in spring wheat. The 117 bp allele of Xgwm533 was found in about 35 % of the cultivars analysed, however, none of them possessed the expected disease resistance.…”

Positioning of the major locus for Puccinia psidii rust resistance (Ppr1) on the Eucalyptus reference map and its validation across unrelated pedigrees

“…QTL validation for different rust diseases caused by Puccinia sp. has been carried out in wheat (Khlestkina et al 2007;Schnurbusch et al 2004), barley (Castro et al 2003), and sugar cane (McIntyre et al 2005) to name a few. In all cases a major locus conferring resistance to the rust disease was found and its presence validated across unrelated pedigrees.…”

Analysis of leaf and stripe rust severities reveals pathotype changes and multiple minor QTLs associated with resistance in an Avocet × Pastor wheat population

“…QTLs on 3BS have been identified by Singh et al (2000b), Suenaga et al (2003) and Khlestkina et al (2007) and on 3BL by Lin and Chen (2009). It seems highly likely that the 3BS QTL of Khlestkina et al (2007) and Singh et al (2000b) are at the same locus as they are flanked by a common SSR marker locus (Xgwm533.2). Furthermore, all of the other markers defining these QTLs have close linkage with each other across many genetic maps.…”

“…Furthermore, all of the other markers defining these QTLs have close linkage with each other across many genetic maps. For example, in the ITMI mapping population, the marker locus Xfba190 in Singh et al (2000b) maps closely to marker loci (Xgwm389 and Xgwm533) defining the ) QTL, as well as the marker loci (Xgwm493 and Xgwm533) of the Khlestkina et al (2007) QTL. The markers defining the 3BL locus of Lin and Chen (2009) are not associated in any maps with the 3BS markers.…”