Detection of microvesicle miRNA expression in ALL subtypes and analysis of their functional roles

Abstract

Microvesicles (MVs) are the heterogeneous mixtures of vesicles. MVs released by leukemia cells constitute an important part of the leukemia microenvironment. MVs might act as important reservoirs of microRNAs (miRNAs). It is worth evaluating whether MVs possess some unique miRNA contents that are valuable in understanding the pathogenesis. In this study, we investigated the miRNA expression patterns of Nalm-6-derived MVs, Jurkat-derived MVs and normal cell-derived MVs using miRNA microarrays. The potential target genes regulated by differentially expressed miRNAs were also predicted and analyzed. Results demonstrated that 182 miRNAs and 166 miRNAs were differentially expressed in Nalm-6-MVs and Jurkat-MVs, respectively. Many oncogenes, tumor suppressors and signal pathway genes were targeted by these aberrantly expressed miRNAs, which might contribute to the development of B-ALL or T-ALL. Our findings expanded the potential diagnostic markers of ALL and provided useful information for ALL pathogenesis.

miRNAs in Acute Lymphoblastic Leukemia: Diagnosis, Prognosis and Target Therapeutic

“…ALL is also the hematology neoplasia most commonly observed in the pediatric population, while it is relatively less common than AML in adults [36]. Around 75% of childhood ALL cases contain at least one alteration chromosomic, have lymphoid maturation arrest in distinct stages, and involve B-or T-lineages to leaving different immunophenotypes with different miRNA signatures [37]. microRNAs participate in different physiological and cellular processes, such as development and tissue differentiation, cell identity, cell cycle progression, and programmed cell death [38].…”

Physiology and pathology of extracellular vesicules

“…ВВ определенных типов можно применять для изби-рательной детекции в конкретных случаях: так, эндо-телиальные микровезикулы успешно показали себя как прогностический маркер развития острой реакции «трансплантат против хозяина» при аллогенной транс-плантации гемопоэтических стволовых клеток [48]. Другой пример -анализ профиля миРНК в циркули-рующих ВВ пациентов с острым лимфобластным лейкозом, позволяющий различить подтипы заболе-вания [49]: предварительные исследования показали, что ВВ из клеток Т-клеточного и В-клеточного остро-го лимфобластного лейкоза обладают принципиально разным профилем миРНК. Аналогичные исследова-ния сейчас проводятся для широкого круга онкологи-ческих и онкогематологических заболеваний, так как микровезикулы плазмы или сыворотки представляют собой очень привлекательный вариант «жидкой биоп-сии».…”

The functional role of microRNA in acute lymphoblastic leukemia: relevance for diagnosis, differential diagnosis, prognosis, and therapy

“…ALL is a lymphoid malignancy, and around three quarters of childhood ALL cases contain one or more total alterations of chromosome, and it may involve B- or T- lineages and have lymphoid maturation arrest in distinct stages, leaving different immunophenotypes with different miRNA signatures. 53 – 57 So, these signatures can help the diagnosis and classification diagnosis of ALL. Compared with normal pediatric BM samples, miRNA-100, miRNA-196b, and let-7e were expressed at a lower level in BM samples of pediatric ALL, while miRNA-128a and miRNA-181b were overexpressed.…”