Detection of metabolite induction in fungal co-cultures on solid media by high-throughput differential ultra-high pressure liquid chromatography–time-of-flight mass spectrometry fingerprinting

Bertrand, Samuel; Schumpp, Olivier; Bohni, Nadine; Bujard, Alban; Azzollini, Antonio; Monod, Michel; Gindro, Katia; Wolfender, Jean‐Luc

doi:10.1016/j.chroma.2013.01.098

Cited by 101 publications

(111 citation statements)

References 57 publications

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“…To study their cellular biology in their native growth environment, they are often cultured on soft solid growth substrates (Bertrand et al, 2013), like many other prokaryotic (Geesey and White, 1990) and eukaryotic cell populations (Solon et al, 2007). Colonies under these conditions grow in three dimensions, with non-uniform thickness and hence the most realistic understanding of fungal expansion and invasion needs a 3D live-colony morphomechanical analysis.…”

Section: Introductionmentioning

confidence: 99%

Quantitative acoustic contrast tomography reveals unique multiscale physical fluctuations during aflatoxin synthesis in Aspergillus parasiticus

Banerjee

Gummadidala

Rima

et al. 2014

Fungal Genetics and Biology

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Section: Introductionmentioning

confidence: 99%

Quantitative acoustic contrast tomography reveals unique multiscale physical fluctuations during aflatoxin synthesis in Aspergillus parasiticus

Banerjee

Gummadidala

Rima

et al. 2014

Fungal Genetics and Biology

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“…Ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS) is considered as one of the most powerful tools to analyze metabolites because of the fast separation, high sensitivity, and excellent resolution171819. Here, we present our work establishing a searchable chemical fingerprint database of marine bacteria using UPLC-HRMS, together with a software package that performs LC-MS alignment and fingerprint searching.…”

mentioning

confidence: 99%

A High-Resolution LC-MS-Based Secondary Metabolite Fingerprint Database of Marine Bacteria

Liang

Wang

Xü

et al. 2014

Sci Rep

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Marine bacteria are the most widely distributed organisms in the ocean environment and produce a wide variety of secondary metabolites. However, traditional screening for bioactive natural compounds is greatly hindered by the lack of a systematic way of cataloguing the chemical profiles of bacterial strains found in nature. Here we present a chemical fingerprint database of marine bacteria based on their secondary metabolite profiles, acquired by high-resolution LC-MS. Till now, 1,430 bacterial strains spanning 168 known species collected from different marine environments were cultured and profiled. Using this database, we demonstrated that secondary metabolite profile similarity is approximately, but not always, correlated with taxonomical similarity. We also validated the ability of this database to find species-specific metabolites, as well as to discover known bioactive compounds from previously unknown sources. An online interface to this database, as well as the accompanying software, is provided freely for the community to use.

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“…examined co-culture of filamentous fungi grown on solid media. 200 Analysis of 657 co-cultures and associated monoculture extracts revealed four distinct types of morphological interactions. A total of 138 representative co-culture extracts, in addition to monoculture extracts were subjected to UHPLC-TOF-MS, feature finding, and subsequent PCA and PLS analyses.…”

Section: Advances Towards Understanding Microbial Interactionsmentioning

confidence: 99%

Symbiosis-inspired approaches to antibiotic discovery

Adnani

Rajski

2017

Nat. Prod. Rep.

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Life on Earth is characterized by a remarkable abundance of symbiotic and highly refined relationships among life forms. Defined as any kind of close, long-term association between two organisms, symbioses can be mutualistic, commensalistic or parasitic. Historically speaking, selective pressures have shaped symbioses in which one organism (typically a bacterium or fungus) generates bioactive small molecules that impact the host (and possibly other symbionts); the symbiosis is driven fundamentally by the genetic machineries available to the small molecule producer. The human microbiome is now integral to the most recent chapter in animal-microbe symbiosis studies and plant-microbe symbioses have significantly advanced our understanding of natural products biosynthesis; this also is the case for studies of fungal-microbe symbioses. However, much less is known about microbe-microbe systems involving interspecies interactions. Microbe-derived small molecules (i.e. antibiotics and quorum sensing molecules, etc.) have been shown to regulate transcription in microbes within the same environmental niche, suggesting interspecies interactions whereas, intraspecies interactions, such as those that exploit autoinducing small molecules, also modulate gene expression based on environmental cues. We, and others, contend that symbioses provide almost unlimited opportunities for the discovery of new bioactive compounds whose activities and applications have been evolutionarily optimized. Particularly intriguing is the possibility that environmental effectors can guide laboratory expression of secondary metabolites from “orphan”, or silent, biosynthetic gene clusters (BGCs). Notably, many of the studies summarized here result from advances in “omics” technologies and highlight how symbioses have given rise to new anti-bacterial and antifungal natural products now being discovered.

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Detection of metabolite induction in fungal co-cultures on solid media by high-throughput differential ultra-high pressure liquid chromatography–time-of-flight mass spectrometry fingerprinting

Cited by 101 publications

References 57 publications

Quantitative acoustic contrast tomography reveals unique multiscale physical fluctuations during aflatoxin synthesis in Aspergillus parasiticus

Quantitative acoustic contrast tomography reveals unique multiscale physical fluctuations during aflatoxin synthesis in Aspergillus parasiticus

A High-Resolution LC-MS-Based Secondary Metabolite Fingerprint Database of Marine Bacteria

Symbiosis-inspired approaches to antibiotic discovery

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