Detection of Matrix Metalloproteinases Patterns in Bovine Luteum cell during Pregnancy

Kim, Sang‐Hwan; Kim, Kyong-Lae; Lee, Jihye; Shin, Da‐Hye; Jung, Na-Hyeon; Lee, Hojun; Yoon, and Jong-Taek

doi:10.12750/jet.2018.33.2.61

Cited by 6 publications

(6 citation statements)

References 13 publications

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“…According to recent studies, inhibition of intracellular mTOR in follicle development and tissue composition may lead to cell starvation, leading to inflammation, which may lead to intracellular necrosis (Kim et al, 2018;Lee et al, 2019;Choi et al, 2020). One process that controls such an inflammatory response leads to TNF/ P53 signaling, which is the general logic of accelerating cell death and reconstructing tissue by the action of intracellular matrix metalloproteinases (Xie and Klionsky, 2007;Kim et al, 2018). However, although a cell death In particular, this phenomenon is considered to have a very positive effect on muscle cells compared with skin cells.…”

Section: Discussionmentioning

confidence: 99%

“…However, studies on whether artificial regulation of apoptosis is possible in the process of cell development and tissue formation and the relationship between artificial regulation and cell development are very lacking. The reason is that the action of apoptosis is formed in a specific cell, and the composition of the detached tissue is reorganized according to the generation of other cells (Kim et al, 2013;2018). However, such a result is a natural process achieved by the supply of sufficient nutrition.…”

Section: Introductionmentioning

confidence: 99%

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Effects of programmed cell death induction method on somatic cell development

Kim

2021

J Anim Reprod Biotechnol

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In this study, to analyze whether artificial regulation of apoptosis in the development of somatic cells can affect the stable growth and development of cells, 20 alpha-hydroxysteroid dehydrogenase (20α-HSD) and rapamycin were treated to induce apoptosis and autophagy in the both skin and muscle cells. Respectively, and 3-methyladenine was supplemented to inhibit cell death. Our results show that stimulation with rapamycin activated autophagy in both types of cells, but increased apoptosis more than autophagy in the case of skin cells. These results indicate that there was a difference in the expression of survival factors and cell development depending on the type of cell. In particular, in the expression of autophagy-related gene (MAP1LC3A) was higher than that of Casp-3, an apoptosis factor. Furthermore, cell development was the highest in all cell groups cultured by artificially inducing autophagy, however the lowest in the apoptosis-inhibited group. Especially, the noteworthy result of this study was that when apoptosis was induced using 20α-HSD, it was possible to induce apoptosis in both skin and muscle cells. Therefore, the main point of this study is that apoptosis induced during cell culture plays a pivotal role in cell remodeling.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of programmed cell death induction method on somatic cell development

Kim

2021

J Anim Reprod Biotechnol

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“…Hlaing, Nam, Lou, Pope, and Nephew () reported that oestrogen receptor cofactors, SRC‐1, GRIP1, RAC3, p300 and RIP140, are more strongly expressed in oestrous cycle than in the luteal phase in domesticated animals. A recent report indicated that GRIP1 protein was differentially expressed between spontaneous and induced oestrus in bovine vaginal mucus (Kim et al., ). Interestingly, our results also showed a significant difference in GRIP1 gene and protein expression among the oestrous stages.…”

Section: Discussionmentioning

confidence: 99%

Identification of lactoferrin and glutamate receptor‐interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection

Lee

Park

Song

et al. 2016

Reprod Domestic Animals

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Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous-specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non-oestrous and controlled internal drug release (CIDR)-induced oestrous-stage mucus proteins were purified and subjected to surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI-TOF/TOF. Among differentially expressed proteins, lactoferrin (LF) and glutamate receptor-interacting protein 1 (GRIP1) showed a twofold increase during the CIDR-induced oestrous stage compared to the levels in non-oestrous stage in bovine cervical mucus. The RT-PCR, Western blotting and immunohistochemistry results showed that LF and GRIP1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine LF and GRIP1 exist during the oestrous stage, but not during the non-oestrous stage, suggesting that cervical mucus LF and GRIP1 are useful oestrous detection markers in cattle.

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“…The corpus luteum cells were isolated and cultured according to the method described by Kim et al (2018). The isolated corpus luteum tissue (30 days) was washed twice with Dulbecco's phosphate buffered saline (Gibco, Carlsbad, CA, USA) containing 300 IU/ml penicillin (Gibco, Carlsbad, CA, USA) and 300 μg/ml streptomycin (Gibco, Carlsbad, CA, USA), and then cut finely with scissors.…”

Section: Corpus Luteum Cell Isolation and Culturementioning

confidence: 99%

Effects of apoptosis by 20α-hydroxysteroid dehydrogenase activity on corpus luteum formation during early pregnancy in cattle

Lee¹,

Cho²,

Kim³

2023

Czech J. Anim. Sci.

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20α-hydroxysteroid dehydrogenase (20α-HSD) is a member of the aldo-keto reductase family. These enzymes play a pivotal role in regulating steroid hormones, such as androgens, oestrogen, and progesterone, and are, therefore, considered vital targets for determining whether a pregnancy is maintained. In this study, we investigated the association between 20α-HSD and apoptosis-related genes in luteal tissues (at 30, 60, and 90 days of gestation) and cells from early gestation in cattle. The corpus luteum altered the number of large lutein cells from 30-90 days. The change in the junction of the connective tissue appeared to affect the density of the corpus luteum. In addition, 20α-HSD was detected in the corpus luteum and cultured cells during early pregnancy, in contrast to the results of previous studies. The overall expression pattern of the 20α-HSD and Casp-3 proteins was lower on day 50 of gestation than on days 30 and 90. However, the 20α-HSD expression gradually increased from 30 to 90 days of gestation. When the 20α-HSD protein was increased to 0.5 μg/ml, 1 μg/ml, and 1.5 μg/ml in the luteal cells collected on day 30 of pregnancy, apoptosis was analysed after 48 hours. 20α-HSD generated in the cells was confirmed, and the concentration gradually decreased as the concentration increased. However, the expression of Casp-3 showed an overall similar expression pattern. Notably, the 20α-HSD and Casp-3 proteins were lowest at 1.5 μg/ml supplemented with 20α-HSD, with higher levels in the cytosol than in the cytoplasm. These results suggest that 20α-HSD plays a role in maintaining normal pregnancy, particularly by regulating the progesterone concentrations during luteal cell development.

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Detection of Matrix Metalloproteinases Patterns in Bovine Luteum cell during Pregnancy

Cited by 6 publications

References 13 publications

Effects of programmed cell death induction method on somatic cell development

Effects of programmed cell death induction method on somatic cell development

Identification of lactoferrin and glutamate receptor‐interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection

Effects of apoptosis by 20α-hydroxysteroid dehydrogenase activity on corpus luteum formation during early pregnancy in cattle

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