Detection of Maedi-Visna Virus in the Kidneys of Naturally Infected Sheep

Angelopoulou, Katerina; Brellou, Georgia; Vlemmas, I.

doi:10.1016/j.jcpa.2006.01.002

Cited by 25 publications

(25 citation statements)

References 22 publications

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“…The histopathologic findings of the present study are comparable to those reported earlier (Angelopoulou et al 2006;Christodoulopoulos 2006;Woldemeskel and Tibbo 2010). Comparison of histopathological findings and PCR results showed that LTR-PCR is highly specific and sensitive in detecting the MVV provirus in the lung samples of sheep.…”

Section: Discussionsupporting

confidence: 88%

“…Therefore, in live animals, virus isolation can be conducted on peripheral blood, milk and inhalation of infected alveolar macrophages (Christodoulopoulos 2006;Gil et al 2006;McNeilly et al 2008;Barquero et al 2011). At necropsy, MVV can be isolated from the lungs, mediastinal lymph nodes, spleen, third eyelids and kidneys of affected animals (Capucchio et al 2003;Angelopoulou et al 2006;Benavides et al 2006;Laamanen et al 2007). Several studies have described the application of PCR methods as the rapid laboratory diagnostic tests in detecting the provirus in blood and other tissues; however, very few estimates of sensitivity and specificity of these methods have been made.…”

Section: Discussionmentioning

confidence: 99%

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Maedi in slaughtered sheep: A pathology and polymerase chain reaction study in southwestern Iran

Azizi

Tajbakhsh

Fathi

et al. 2011

Trop Anim Health Prod

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Maedi-visna (MV) is an important slow viral disease of sheep leading to a progressive lymphoproliferative disease. It affects multiple organs primarily the lungs, where it causes interstitial pneumonia (maedi). In this study, the lungs of 1,000 sheep carcasses were grossly inspected and those suspected to have maedi were studied at histopathological and molecular levels. A polymerase chain reaction (PCR) technique that amplified a 291-base pair DNA in the long terminal repeat (LTR) sequence of MV provirus was conducted on all the 50 suspected lungs together with 10 normal appearing lungs as controls. Amplicons of the expected size were detected in 11 (n=11/50) suspected sheep, and one of the 10 control sheep. Histopathologic study of the pulmonary lesions of all 11 (n=11/11) positive sheep showed MV lesions, including hyperplasia of the perivascular and peribronchiolar lymphoid cells, interstitial lymphoplasmacytic infiltration and smooth muscle hyperplasia and the histopathologic findings were correlated with PCR results. In contrast, the tissue sections of control animals were almost normal at histopathological level; however, PCR technique demonstrated that one of them was affected by maedi. This study showed that the LTR-PCR had high specificity and sensitivity in diagnosis of this viral infection. This study is the first to evaluate the prevalence of MV virus infection in sheep in Iran.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Maedi in slaughtered sheep: A pathology and polymerase chain reaction study in southwestern Iran

Azizi

Tajbakhsh

Fathi

et al. 2011

Trop Anim Health Prod

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“…In recent years some studies have revealed MVV in different organs and cell types (Capucchio et al 2003;Carrozza et al 2003;Preziuso et al 2003;Angelopoulou et al 2006;Brellou et al 2007). It is well known that lymph nodes are target for some Lentivirus infections, such as HIV and FIV, while little information is available on MVV infection in these sites.…”

Section: Discussionmentioning

confidence: 99%

“…liver, heart, spleen, bone marrow, and third eyelids of infected sheep (Capucchio et al 2003;Preziuso et al 2003;Angelopoulou et al 2006;Brellou et al 2007). The viral antigen p25 has also been detected in pulmonary and supramammary lymph nodes of sheep experimentally infected with pulmonary and mammary lesions, independently from the severity of the lesions present in the drained organs (Preziuso et al 2003).…”

mentioning

confidence: 99%

Detection of the Maedi Visna Virus in the popliteal lymph nodes of sheep infected by the respiratory route

et al. 2009

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“…Lymphoproliferative lesions have been reported mainly in lungs, the central nervous system, udder and joints (Pepin et al 1998). However, the virus has been detected also in other organs, such as the kidney, the liver, the heart and the third eyelid (Capucchio et al 2003;Angelopoulou et al 2006;Brellou et al 2007). There is a paucity of data regarding VMV infection in lymphoid organs.…”

mentioning

confidence: 99%

Detection of Visna Maedi virus in mesenteric lymph nodes and in other lymphoid tissues of sheep three years after respiratory infection

Preziuso¹,

Magi²,

Mari³

et al. 2013

Vet. Med. - Czech

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Visna/Maedi virus (VMV), a small ruminant lentivirus responsible for lymphoproliferative pneumonia, encephalitis, arthritis and/or mastitis in sheep, has been detected in different non-lymphoid organs. However, only a few investigations have been carried out in lymphoid tissues. In this study, some lymphoid tissues and lymph node draining or non-draining VMV target organs from five sheep infected experimentally by the respiratory route three years previously were investigated. Archival samples of spleen, red bone marrow, caudal mediastinal lymph nodes, mammary lymph nodes, popliteal lymph nodes and mesenteric lymph nodes were tested by PCR for the presence of proviral DNA. Popliteal and mesenteric lymph node samples were tested also by immunohistochemical staining of the viral capsid antigen p28. The proviral DNA was detected by PCR in all the lymphoid tissue samples from the infected sheep. The viral antigen was stained in mononuclear cells in popliteal and mesenteric lymph nodes of the infected sheep. Although the lymph nodes draining the classical target organs seem to be more infected than the others, both the viral capsid antigen and the proviral DNA were present also in lymph nodes draining non-target organs, such as the mesenteric lymph nodes. These findings show the presence of VMV in different lymphoid tissues in the late stages of infection and suggest a potential role of these tissues as a site for viral reservoir and replication, even three years after infection.

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Detection of Maedi-Visna Virus in the Kidneys of Naturally Infected Sheep

Cited by 25 publications

References 22 publications

Maedi in slaughtered sheep: A pathology and polymerase chain reaction study in southwestern Iran

Maedi in slaughtered sheep: A pathology and polymerase chain reaction study in southwestern Iran

Detection of the Maedi Visna Virus in the popliteal lymph nodes of sheep infected by the respiratory route

Detection of Visna Maedi virus in mesenteric lymph nodes and in other lymphoid tissues of sheep three years after respiratory infection

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