Detection of the Maedi Visna Virus in the popliteal lymph nodes of sheep infected by the respiratory route

Preziuso, S.; Magi, Gian Enrico; Valente, C.; Cuteri, Vincenzo

doi:10.1007/s11259-009-9262-z

Cited by 3 publications

(3 citation statements)

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“…neg. = negative; sPCR = positive in first-round (single PCR) and in second-round PCR (nested PCR); nPCR = positive only in second-round PCR (nested PCR) *sample tested in a previous work (Preziuso et al 2009) and positive by sPCR The finding of both the proviral DNA and the viral capsid antigen in the mesenteric lymph nodes of sheep three years after infection by the respiratory route was unexpected. In only one case was a weak positivity in these lymph nodes reported in two lambs a few weeks after pulmonary inoculation (Gendelman et al 1985).…”

Section: Discussionmentioning

confidence: 97%

“…Samples from three sheep (numbers 2, 5 and 7) inoculated with VMV were unavailable for this study. Preliminary results on the popliteal lymph node of sheep 1 were excluded from this experiment because they had been used in a previous preliminary study together with popliteal lymph node samples from sheep 2, 5 and 7 (Preziuso et al 2009). …”

Section: Methodsmentioning

confidence: 99%

“…Histology and immunohistochemistry. In this study, mammary lymph nodes, popliteal lymph nodes and mesenteric lymph nodes were tested by immunohistochemistry (IHC), while the other lymphoid samples were already tested in other studies (Preziuso et al 2003(Preziuso et al , 2009). The popliteal and mesenteric lymph nodes were also examined for histological lesions, while the histopathological findings in the mammary lymph node samples have been described previously (Preziuso et al 2003).…”

Section: Methodsmentioning

confidence: 99%

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Detection of Visna Maedi virus in mesenteric lymph nodes and in other lymphoid tissues of sheep three years after respiratory infection

Preziuso¹,

Magi²,

Mari³

et al. 2013

Vet. Med. - Czech

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Visna/Maedi virus (VMV), a small ruminant lentivirus responsible for lymphoproliferative pneumonia, encephalitis, arthritis and/or mastitis in sheep, has been detected in different non-lymphoid organs. However, only a few investigations have been carried out in lymphoid tissues. In this study, some lymphoid tissues and lymph node draining or non-draining VMV target organs from five sheep infected experimentally by the respiratory route three years previously were investigated. Archival samples of spleen, red bone marrow, caudal mediastinal lymph nodes, mammary lymph nodes, popliteal lymph nodes and mesenteric lymph nodes were tested by PCR for the presence of proviral DNA. Popliteal and mesenteric lymph node samples were tested also by immunohistochemical staining of the viral capsid antigen p28. The proviral DNA was detected by PCR in all the lymphoid tissue samples from the infected sheep. The viral antigen was stained in mononuclear cells in popliteal and mesenteric lymph nodes of the infected sheep. Although the lymph nodes draining the classical target organs seem to be more infected than the others, both the viral capsid antigen and the proviral DNA were present also in lymph nodes draining non-target organs, such as the mesenteric lymph nodes. These findings show the presence of VMV in different lymphoid tissues in the late stages of infection and suggest a potential role of these tissues as a site for viral reservoir and replication, even three years after infection.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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