Detection of Macular Ganglion Cell Loss in Glaucoma by Fourier-Domain Optical Coherence Tomography

Abstract

Purpose-To map ganglion cell complex thickness with high-speed Fourier-domain optical coherence tomography (FD-OCT) and compute novel macular parameters for glaucoma diagnosis.Design-Observational, cross-sectional study.Participants-One hundred seventy-eight participants in the Advanced Imaging for Glaucoma Study, divided into three groups: 65 persons in the normal group (N), 78 in the perimetric glaucoma group (PG), and 52 in the pre-perimetric glaucoma group (PPG). Methods-TheRTVue FD-OCT system was used to map the macula over a 7×6 mm region. The macular OCT images were exported for automatic segmentation using software we developed. The program measured macular retinal (MR) thickness and ganglion cell complex (GCC) thickness. The GCC was defined as the combination of nerve fiber, ganglion cell, and inner plexiform layers. Pattern analysis was applied to the GCC map and the diagnostic power of pattern-based diagnostic parameters were investigated. Results were compared to time-domain (TD) Stratus OCT measurements of MR and circumpapillary nerve fiber layer (NFL) thickness.Main Outcome Measures-Repeatability was assessed by intraclass correlation (ICC), pooled standard deviation, and coefficient of variation. Diagnostic power was assessed by the area under the receiver operator characteristic (AROC) curve. Measurements in the PG group were the primary measures of performance.Results-The FD-OCT measurements of MR and GCC averages had significantly better repeatability than TD-OCT measurements of MR and NFL averages. The FD-OCT GCC average had significantly (P=0.02) higher diagnostic power (AROC = 0.90) than MR (AROC = 0.85 for both FD-OCT & TD-OCT) in differentiating between PG and N. One GCC pattern parameter, global loss volume, had significantly higher AROC (0.92) than the overall average (P=0.01). The diagnostic powers of the best GCC parameters were statistically equal to TD-OCT NFL average. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.This article contains online-only material. The following should appear online-only: table 3, figures 3 and figure 6. NIH Public Access Author ManuscriptOphthalmology. Author manuscript; available in PMC 2010 December 1. Published in final edited form as:Ophthalmology. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptConclusions-The higher speed and resolution of FD-OCT improved the repeatability of macular imaging compared to standard TD-OCT. Ganglion cell mapping and pattern analysis improved diagnostic power. The improved diagnostic power of macular GCC imaging is on par with, and complementary to...

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