Detection of &lt;i&gt;felis catus&lt;/i&gt; papillomavirus type 3 and 4 DNA          from squamous cell carcinoma cases of cats in Japan

Yamashita‐Kawanishi, Nanako; Sawanobori, Ryoma; Matsumiya, Kosuke; Uema, Akiko; Chambers, James K.; Uchida, Kazuyuki; Shimakura, Hidekatsu; Tsuzuki, Masano; Chang, Chia‐Yu; Chang, Hui‐Wen; Haga, Takeshi

doi:10.1292/jvms.18-0089

Cited by 24 publications

(42 citation statements)

References 24 publications

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“…FcaPV was only detected in 1 of 20 FOSCC samples. These results are consistent with all published literature that includes > 1 FOSCC patient, where PV infection was detected in only 0-6.9% of patients (Munday and French, 2015;Munday et al, 2019Munday et al, , 2009Munday et al, 2011b;O'Neill et al, 2011;Supsavhad et al, 2016;Yamashita-Kawanishi et al, 2018). It is possible that PV was not detected but causes FOSCC via a 'hit and run' mechanism, where E6 and E7 contribute to tumor initiation, the virus is lost in overt neoplasms, and it is not required to maintain the transformed phenotype.…”

Section: Discussionsupporting

confidence: 91%

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A virome sequencing approach to feline oral squamous cell carcinoma to evaluate viral causative factors

Chu

Wylie

et al. 2020

Veterinary Microbiology

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Feline oral squamous cell carcinoma (FOSCC) may be the best naturally-occurring model of human head and neck squamous cell carcinoma (HNSCC). HNSCC can be broadly divided into human papillomavirus (HPV)negative cancers and HPV-positive cancers where HPV is the causative agent. Previous studies in FOSCC have used both species-specific and species-nonspecific PCR primers that may be insensitive to the detection of PVs and other viruses that may be divergent from known sequences. ViroCap is a targeted capture and next generation sequencing tool that was designed to identify all known vertebrate DNA and RNA viruses. In this study we used a metagenomic approach using ViroCap for DNA viruses in 20 FOSCC, 9 normal feline oral mucosal, and 8 suspected PV positive control samples. We tested the hypothesis that viruses would be enriched in FOSCC compared to normal oral mucosa. The virome of the FOSCC and normal feline oral mucosa consisted of feline foamy virus in 7/20 and 2/9 (35% and 22%), feline torque teno virus in 2/20 and 0/9 (10% and 0%), alphaherpesvirus in 2/10 and 0/9 (10% and 0%), FIV (0% and 22%), Epstein-Barr virus in 1/20 and 0/9 (5% and 0%) and feline papillomavirus in 1/20 and 0/9 samples (5% and 0% respectively). Felis catus papillomavirus-3 was found in 1 of 20 FOSCC samples. A virus was not associated consistently with FOSCC. If PVs have a role in FOSCC it is at most a supplementary or uncommon role. FOSCC appears most closely related to HPV-negative HNSCC. Future research on FOSCC should focus on identifying genetic and environmental causes. (S. Chu). Veterinary Microbiology 240 (2020) 108491 0378-1135/ Published by Elsevier B.V.T

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Section: Discussionsupporting

confidence: 91%

“…FcaPV-3 is not as commonly isolated as FcaPV-2 in cats ( Supplementary Table 1). FcaPV-3 infection has been found in cutaneous SCC, nasal planum SCCs and BISC lesions (Munday et al, 2013a(Munday et al, , 2016Munday et al, 2013b, a;Yamashita-Kawanishi et al, 2018). FcaPV-3 could have been an incidental infection.…”

Section: Discussionmentioning

confidence: 98%

A virome sequencing approach to feline oral squamous cell carcinoma to evaluate viral causative factors

Chu

Wylie

et al. 2020

Veterinary Microbiology

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“…FcaPV-2 has not been detected neither in skin nor in oral SCC: in this case, a lower prevalence of this virus in that geographical area or less extensive sampling might be a possible explanation for the discrepancy with our results. 28,29 In situ hybridization (ISH) is considered the standard tool to detect FcaPV-2 DNA within neoplastic cells. 3,22 We attempted at performing ISH procedures on our samples, however, results were not conclusive.…”

Section: Discussionmentioning

confidence: 99%

Detection of Felis catus papillomavirus type‐2 DNA and viral gene expression suggest active infection in feline oral squamous cell carcinoma

Altamura

Cardeti

Cersini

et al. 2020

Vet Comparative Oncology

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Papillomavirus (PV) infection is associated with development of epithelial cancer in different species, including domestic cat (Felis catus). Felis catus PV type-2 (FcaPV-2) is considered the causative agent of a proportion of feline cutaneous squamous cell carcinoma (SCC), through the transforming properties of its E6 and E7 oncogenes. However, the possible role of FcaPVs in the aetiology of feline oral SCC (FOSCC) is still unclear. The aim of this study was to assess the presence and gene expression of FcaPV-2 in FOSCC samples. We detected FcaPV-2 DNA in 10/32 (31%) of the analysed FOSCC by the use of PCR methods. Importantly, viral mRNA was detected by RT-PCR in 7/10 (70%) of DNA positive samples. In particular, FcaPV-2 L1, E2 and E6E7 genes were found to be expressed in 5/10 (50%), 3/10 (33%) and 5/10 (50%) samples, respectively. Viral DNA was also detected in non neoplastic oral ulcerative lesions (ULs) (4/11, 36%); qPCR suggested a difference in viral load between ULs and FOSCCs, particularly in those expressing E6E7, although it was not statistically significant. These data suggest, but do not definively prove, a possible role of FcaPV-2 in the development of a proportion of FOSCC. Moreover, L1 and E2 gene expression results indicate that FcaPV-2 infection associated with these tumours may possibly be productive.

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“…The degenerative primer set, CP4/5, used in the present study, is one of the most commonly used primer sets for detecting papillomavirus and searching for novel papillomaviruses. It has been successfully used to detect over 60 types of HPVs (HPVs 1-8, HPVs 10-19, HPVs 21-26, HPVs 30-38, HPV 40, HPVs 45-47, and HPV 60) [44], seven types of CPVs (CPVs 1-7) [34], four types of FcaPV (1, 3, 4, and 5), and other PVs from wild animals [42,[45][46][47]. In the present study, the successful amplification of CPV 1, 2, 6, 9, 15, and CPV 16 by using the CP4/5 primers suggests the broader application of this primer set in CPVs.…”

Section: Discussionmentioning

confidence: 99%

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

Chang

Chen

Haga

et al. 2020

Viruses

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Papillomavirus (PV) mainly infects the squamous epithelium and may potentially lead to benign or even malignant cutaneous lesions. However, the malignant transforming ability has been identified in several types of PVs. In humans, papillomavirus (HPV) type 16 and 18 are the most prevalent causative agents of cervical cancer. Therefore, vaccines are being developed to protect against these types. For dogs, there have been limited investigations into the association of different canine papillomavirus (CPV) genotypes with malignant lesions. Understanding the high-risk CPV genotype(s) responsible for these malignant lesions would contribute to the development of interventions for preventing CPV-induced carcinomas. In the present study, a retrospective cohort of 102 pathologically confirmed papillomas and 212 squamous cell carcinomas (SCCs) were included. The viral genome and antigens in the formalin-fixed paraffin-embedded (FFPE) tissues were detected using PCR targeting pan PV E1 and COPV L1 genes and by immunohistochemistry staining (IHC), respectively. PVs were successfully detected from 11 FFPE cutaneous tissues and four oral tissues using pan PV E1- and COPV L1-based PCR, respectively. After sequencing, CPV 1, CPV 2, and CPV 6 were detected in the benign lesions using PCR and were confirmed through IHC. While CPV 9 and CPV 15 were first detected in the SCCs of dogs, CPV 16 was most often detected in SCC specimens. The association and confirmative demonstration of viral genes and intralesional antigens of CPV 9, CPV 15, and CPV 16 in SCCs highlight the potential risk of these genotypes of CPVs in malignant transformation.

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Detection of <i>felis catus</i> papillomavirus type 3 and 4 DNA from squamous cell carcinoma cases of cats in Japan

Cited by 24 publications

References 24 publications

A virome sequencing approach to feline oral squamous cell carcinoma to evaluate viral causative factors

A virome sequencing approach to feline oral squamous cell carcinoma to evaluate viral causative factors

Detection of Felis catus papillomavirus type‐2 DNA and viral gene expression suggest active infection in feline oral squamous cell carcinoma

The Detection and Association of Canine Papillomavirus with Benign and Malignant Skin Lesions in Dogs

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