Detection of lipid peroxidation in lung and in bronchoalveolar lavage cells and fluid

Petruska, Janet; Wong, Steven H.; Sunderman, F. William; Mossman, Brooke T.

doi:10.1016/0891-5849(90)90049-o

Cited by 35 publications

(7 citation statements)

References 23 publications

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“…Lungs evaluated immediately after the equilibration period before production of ischemia showed values for tissue TBARS, ATP, and dry/wet weight (Table I) similar to previously published values for perfused lungs (15,18). Control lungs which were perfused for an additional 60 min (no ischemia) and ventilated with either 20% or 95% 02 showed no significant change in tissue TBARS, conjugated dienes, ATP, or dry/wet weight (Table I).…”

Section: Resultssupporting

confidence: 72%

Oxygen-dependent lipid peroxidation during lung ischemia.

Fisher

Dodia²,

Tan³

et al. 1991

J. Clin. Invest.

142

101

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The effect of alveolar oxygen tension on lung lipid peroxidation during lung ischemia was evaluated by using isolated rat lungs perfused with synthetic medium. After a 5-min equilibration period, global ischemia was produced by discontinuing perfusion while ventilation continued with gas mixtures containing 5% CO2 and a fixed oxygen concentration between 0 and 95%. Lipid peroxidation was assessed by measurement of tissue thiobarbituric acid-reactive products and conjugated dienes. Control studies (no ischemia) showed no change in parameters of lipid peroxidation during 1 h of perfusion and ventilation with 20% or 95% 02. With 60 min of ischemia, there was increased lipid peroxidation which varied with oxygen content of the ventilating gas and was markedly inhibited by ventilation with N2. Perfusion with 5-, 8-, 11-, 14-eicosatetraynoic acid indicated that generation of eicosanoids during ischemia accounted for -40-50% of lung lipid peroxide production. Changes of CO2 content of the ventilating gas (to alter tissue pH) or of perfusate glucose concentration had no effect on lipid peroxidation during ischemia, but perfusion at 8% of the normal flow rate prevented lipid peroxidation. Lung dry/wet weight measured after 3 min of reperfusion showed good correlation between lung fluid accumulation and lipid peroxidation. These results indicate that reperfusion is not necessary for lipid peroxidation with ischemic insult of the lung and provide evidence that elevated Po2 during ischemia accelerates the rate of tissue injury. (J. Clin. Invest. 1991. 88:674479.)

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Section: Resultssupporting

confidence: 72%

Oxygen-dependent lipid peroxidation during lung ischemia.

Fisher

Dodia²,

Tan³

et al. 1991

J. Clin. Invest.

142

101

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“… 8 Malondialdehyde is such an aldehyde that can be used to evaluate lipid peroxidation in serum, other body fluids including BAL fluid and tissues. 3,9 …”

Section: Discussionmentioning

confidence: 99%

“…This method was reported to be an accurate and sensitive method for measurement of BAL fluid also. 3 …”

Section: Methodsmentioning

confidence: 99%

Changes in malondialdehyde levels in bronchoalveolar fluid and serum by the treatment of asthma with inhaled steroid and beta₂‐agonist

et al. 2000

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“…Although ROS have been viewed classically as injurious to many cell types via modification of macromolecules such as DNA, the effects of oxidants on cells are complex and doserelated. Oxidants generated by fibrogenic dusts or cigarette smoke may induce uptake of a variety of particle types (reviewed in reference 107), lipid peroxidation (115,116), stimulation of cell-signaling cascades and transcription factors (93,117,118), and release of cytokines such as tumor necrosis factor-alpha (TNF-␣) (119). That these interrelated oxidant-induced events are important in inflammation and fibrogenesis is substantiated by successful attempts to inhibit lung disease in rodent inhalation and intratracheal injection models using antioxidants and antibodies to TNF (see Section 5).…”

Section: Cellular and Molecular Mechanisms Of Asbestosis And Silicosismentioning

confidence: 99%

Mechanisms in the Pathogenesis of Asbestosis and Silicosis

Mossman

Churg

1998

Am J Respir Crit Care Med

831

568

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Detection of lipid peroxidation in lung and in bronchoalveolar lavage cells and fluid

Cited by 35 publications

References 23 publications

Oxygen-dependent lipid peroxidation during lung ischemia.

Oxygen-dependent lipid peroxidation during lung ischemia.

Changes in malondialdehyde levels in bronchoalveolar fluid and serum by the treatment of asthma with inhaled steroid and beta₂‐agonist

Mechanisms in the Pathogenesis of Asbestosis and Silicosis

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Detection of lipid peroxidation in lung and in bronchoalveolar lavage cells and fluid

Cited by 35 publications

References 23 publications

Oxygen-dependent lipid peroxidation during lung ischemia.

Oxygen-dependent lipid peroxidation during lung ischemia.

Changes in malondialdehyde levels in bronchoalveolar fluid and serum by the treatment of asthma with inhaled steroid and beta2‐agonist

Mechanisms in the Pathogenesis of Asbestosis and Silicosis

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Product

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About

Changes in malondialdehyde levels in bronchoalveolar fluid and serum by the treatment of asthma with inhaled steroid and beta₂‐agonist