Detection of large deletion in human BRCA1 gene in human breast carcinoma MCF-7 cells by using DNA-Silver Nanoclusters

Borghei, Yasaman‐Sadat; Hosseini, Morteza; Ganjali, Mohammad Reza

doi:10.1088/2050-6120/aa8988

Cited by 26 publications

(8 citation statements)

References 49 publications

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“…Transition electron microscopy (TEM) images were not acquired because at the size of these Ag NCs. Typical TEM images do not show the true size of the Ag NCs, rather showing aggregates that are formed during measurement [39,40,41].…”

Section: Resultsmentioning

confidence: 99%

Glucose Sensor Using Redox Active Oligonucleotide-Templated Silver Nanoclusters

2019

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Redox active, photoluminescent silver nanoclusters templated with oligonucleotides were developed for glucose sensing. The silver nanoclusters had a photoluminescent emission at 610 nm that reversibly changed to 530 nm upon oxidation. The reversible emission change was measured with photoluminescent spectroscopy and used to detect H2O2, which is a by-product of the reaction of glucose with glucose oxidase. The ratio of the un-oxidised emission peak (610 nm) and the oxidised analogue (530 nm) was used to measure glucose concentrations up to 20 mM, well within glucose levels found in blood. Also, the reversibility of this system enables the silver nanoclusters to be reused.

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Section: Resultsmentioning

confidence: 99%

Glucose Sensor Using Redox Active Oligonucleotide-Templated Silver Nanoclusters

2019

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“…Large deletion mutations in BRCA1 were discovered using nanocluster fluorescence produced by recognition hybridization. 91 …”

Section: Application Of Nanotechnology For Cancer Diagnosis and Treat...mentioning

confidence: 99%

Nanotechnology: A Promising Approach for Cancer Diagnosis, Therapeutics and Theragnosis

Dessale¹,

Mengistu²,

Mengist³

2022

IJN

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Cancer remains the most devastating disease and the major cause of mortality worldwide. Although early diagnosis and treatment are the key approach in fighting against cancer, the available conventional diagnostic and therapeutic methods are not efficient. Besides, ineffective cancer cell selectivity and toxicity of traditional chemotherapy remain the most significant challenge. These limitations entail the need for the development of both safe and effective cancer diagnosis and treatment options. Due to its robust application, nanotechnology could be a promising method for in-vivo imaging and detection of cancer cells and cancer biomarkers. Nanotechnology could provide a quick, safe, cost-effective, and efficient method for cancer management. It also provides simultaneous diagnosis and treatment of cancer using nano-theragnostic particles that facilitate early detection and selective destruction of cancer cells. Updated and recent discussions are important for selecting the best cancer diagnosis, treatment, and management options, and new insights on designing effective protocols are utmost important. This review discusses the application of nanotechnology in cancer diagnosis, therapeutics, and theragnosis and provides future perspectives in the field.

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“…The DNA sensing method would be based on changes in CNDs fluorescence intensity after interaction with single or double-stranded DNA. Compared to other fluorescent probes used for gene mutation detection, such as Ag nanoclusters [25] or a iridium (III) based complex [26],…”

Section: Choice Of Materialsmentioning

confidence: 99%

Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

et al. 2019

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The authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the fluorescence intensity following interaction with ssDNA and dsDNA were used for specific DNA sequence of BRCA1, CFRT and MRP3 genes detection. The response to DNAs is linear up to 200 nM and the detection limit is 270 pM. The assay selectivity allows the detection of single gene mutations. Under optimum conditions, the assay can rapidly discriminate between wild type and mutated samples.

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Detection of large deletion in human BRCA1 gene in human breast carcinoma MCF-7 cells by using DNA-Silver Nanoclusters

Cited by 26 publications

References 49 publications

Glucose Sensor Using Redox Active Oligonucleotide-Templated Silver Nanoclusters

Glucose Sensor Using Redox Active Oligonucleotide-Templated Silver Nanoclusters

Nanotechnology: A Promising Approach for Cancer Diagnosis, Therapeutics and Theragnosis

Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

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