Detection of Kyuri Green Mottle Mosaic Virus from Soil by the Immunocapture Reverse 
 Transcription Loop-mediated Isothermal Amplification Reaction

Fukuta, Shiro; Takeyama, Keiko; Suzuki, Miwa; Shichi, A.; Ichikawa, Kazuhiro; Nakanishi, Hiroyuki

doi:10.3923/ppj.2012.51.59

Cited by 12 publications

(4 citation statements)

References 24 publications

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“…Similar to other tobamoviruses. KGMMV has a highly stable structure that is easily transmitted by foliage contact, soil contamination, and seed (Daryono et al 2005;Fukuta et al 2012). We demonstrated that local and imported seed lots have a KGMMV infection.…”

Section: Discussionmentioning

confidence: 80%

“…Percentage bootstrap values (greater than 50) are shown on each branch seed trade is introducing damaging virus diseases to parts of the world where they were previously absent. Among the main cucurbit-infecting tobamoviruses, KGMMV has been reported to cause severe reduction in cucurbit plants in several countries (Daryono et al 2005;Tan et al 2000;Fukuta et al 2012). Similar to other tobamoviruses.…”

Section: Discussionmentioning

confidence: 99%

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Kyuri green mottle mosaic virus detected for the first time in Turkey

Balsak

2023

Australasian Plant Dis. Notes

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Turkey is among the top 10 producers of cucumber, melon, watermelon, and squash in the world. Lately, seed-borne viruses have become a major issue in greenhouse and field-grown cucurbits. In this study, the incidence of kyuri green mottle mosaic virus (KGMMV) was determined in seeds from various species (cucumber, melon, watermelon, summer squash, bottle gourd, winter squash) of Cucurbitaceae. KGMMV detection in a total of 20 seed lots of each cucurbit species was done by enzyme-linked immunosorbent assay (ELISA) and RT-PCR. The highest virus incidence was 45% in melon followed by 25% in cucumber and 10% in squash. To the best of our knowledge, this is the first report of KGMMV in cucurbit crops in Turkey and this highlights the potential risk of KGMMV to commercial cucurbit seed lots.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Kyuri green mottle mosaic virus detected for the first time in Turkey

Balsak

2023

Australasian Plant Dis. Notes

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“…The LAMP assay was developed for the detection of plant pathogenic viruses (Fukuta et al . , , ), viroids (Boubourakas et al . ), fungi (Tomlinson et al .…”

Section: Introductionmentioning

confidence: 99%

“…Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that relies on autocycling strand displacement DNA synthesis performed by the Bst DNA polymerase (Notomi et al 2000). The LAMP assay was developed for the detection of plant pathogenic viruses (Fukuta et al 2003(Fukuta et al , 2004(Fukuta et al , 2012, viroids (Boubourakas et al 2009), fungi (Tomlinson et al 2010), bacteria (Rigano et al 2010) and oomycetes pathogen (Tomlinson et al 2007;Dai et al 2012;Fukuta et al 2013a,b). LAMP requires four different primers designed specifically to recognize six distinct regions on the target gene.…”

Section: Introductionmentioning

confidence: 99%

Development of loop-mediated isothermal amplification assay for the detection of Pythium myriotylum

Fukuta¹,

Takahashi²,

Kuroyanagi³

et al. 2014

Lett Appl Microbiol

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Significance and Impact of the Study: This study shows the first LAMP assay for the detection of Pythium myriotylum. The primer set designed from ITS region of P. myriotylum can detect the pathogen in field sample with a fast and convenient method. Analysis of the annealing curve of the LAMP reaction products increases the reliability of the LAMP diagnosis. This study shows that the diagnostic method using the LAMP assay is useful for monitoring P. myriotylum in the field. AbstractThis study reports the development of a loop-mediated isothermal amplification (LAMP) reaction for the detection of Pythium myriotylum. The primer set targeting the ITS sequence of P. myriotylum worked most efficiently at 60°C and allowed the detection of P. myriotylum DNA within 30 min by fluorescence monitoring using a real-time PCR instrument. The peak denaturing temperature of amplified DNA was about 87Á0°C. In specificity tests using eight Pythium myriotylum strains, 59 strains from 39 species of Pythium, 11 Phytophthora strains and eight other soil-borne pathogens, LAMP gave no cross-reactions. The detection limit was 100 fg of genomic DNA, which was as sensitive as PCR. LAMP could detect P. myriotylum in hydroponic solution samples, and the results coincided with those of the conventional plating method in almost all cases. The LAMP method established in this study is a simple and sensitive tool for the detection of P. myriotylum.

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