Detection of Integrons and Staphylococcal Cassette Chromosome ( SCCmec ) Types in Staphylococcus Aureus Isolated from Burn and Non-Burn Patients

Namvar, Amirmorteza Ebrahimzadeh; Khodaei, Farzaneh; Bijari, Aslan; Lari, Abdolaziz Rastegar

doi:10.12816/0031234

Cited by 3 publications

(5 citation statements)

References 21 publications

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“…Therefore, these options can be recommended for the management of staphylococcal infections in our region by considering the cost-effectiveness of the used antibiotics. Although the antibiotic resistance pattern of S. aureus can be varied in different geographic regions or even within hospitals in the same region, there are several results in Iran and other countries comparable with our findings (22)(23)(24).…”

Section: Discussionsupporting

confidence: 88%

The First Report of Prevalence of Class 1-3 Integrons in Clinical Isolates of Staphylococcus aureus in Southwestern Iran: A Multicenter Study

Hosseini

Hadi

Bazargani

et al. 2020

Jundishapur J Microbiol

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Background: Staphylococcus aureus is one of the main causes of a wide range of nosocomial infections, like the infection of the skin and soft tissue. Objectives: The present study aimed to investigate the occurrence of antibiotic resistance and the presence of class 1-3 integrons in S. aureus strains isolated from hospitalized patients. Methods: This cross-sectional study was carried out during six months in two major hospitals in southwestern Iran. Totally, 134 isolates of S. aureus were identified by phenotypic and molecular methods. A disk diffusion method was used to determine the antibiotic resistance pattern of S. aureus isolates. The detection of integron-encoding genes was performed by the PCR method. Results: Based on antibiotic susceptibility testing, 58 (43.2%) of 134 isolates of S. aureus were reported as methicillin-resistant S. aureus (MRSA) using cefoxitin disc diffusion and mecA gene detection. The highest resistance was seen to tetracycline (48.5%). The results were analyzed by the chi-square test and there was a significant difference (P value < 0.05) between antibiotic resistance in methicillin-sensitive S. aureus (MSSA) isolates. Molecular analysis showed that 53 (39.6%), 5 (3.7%), and none of the isolates were positive for class 1, 2, and 3 integrons, respectively. Conclusions: The results indicated the remarkable rate of class 1 integrons in the clinical isolates of S. aureus in our region and the importance of investigating the prevalence of antibiotic resistance among them. However, these data alone cannot confirm the association between integrons and the presence of antibiotic resistance genes.

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Section: Discussionsupporting

confidence: 88%

The First Report of Prevalence of Class 1-3 Integrons in Clinical Isolates of Staphylococcus aureus in Southwestern Iran: A Multicenter Study

Hosseini

Hadi

Bazargani

et al. 2020

Jundishapur J Microbiol

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“…The SCC mec typing of 13 MRSA strains showed that 9 (69.2%) strains belonged to SCC mec III, that's almost is consistent with some studies in Iran; 56.8% (2), 47.5% (33), 70.1% (15), and 71.7% (16) did not match the other studies in Iran; 95.7% (30), China; 95.4% (16), Brazil; 94.8% (34). 7.7% of the these 13 strains belonged to SCC mec II, compared with 10% (33) and 2.5% (15) studies in Iran, and (1%) in Brazil (34).…”

Section: Discussionsupporting

confidence: 87%

“…7.7% of the these 13 strains belonged to SCC mec II, compared with 10% (33) and 2.5% (15) studies in Iran, and (1%) in Brazil (34). Although 3 (23.1%) other types were not detected in this study, SCC mec I, IV, and V have been detected in other studies (2, 3, 15, 16, 34).…”

Section: Discussioncontrasting

confidence: 68%

“…According to the antibiotic susceptibility testing of all 31 strains, the highest resistance was related to erythromycin (48.4%) compared to 58.2% (2), 77.8% (30), and 90.6% (16) in Iran. All the 13 MRSA strains in this study were sensitive to linezolid, which was similar to the findings of the studies conducted by Montazeri (31, 32), and Goudarzi (16) from Iran and Murray (7) from the US.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the skin damaged and weakened innate immunity, burn patients become susceptible to various infections (1, 2), among which bacterial infections are the main problems in hospitalized burn patients (3, 4).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Community-Associated Methicillin-Resistant Staphylococcus aureus in Iranian Burn Patients

et al. 2019

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Background & Objective:Methicillin-resistant Staphylococcus aureus (MRSA) is reported as one of the important bacterial causes of burn wound infections. This study was carried out to investigate molecular characterization of community-associated MRSA (CA-MRSA) isolated from Iranian burn patients. Methods:A total of 31 isolates of S. aureus were collected from the Motahari Burns Hospital (Tehran, Iran) in 2016. All isolates were collected from outpatients and inpatients within 48 hours of admission. The mecA, pvl, tsst-1, hla-α, and psmα genes detecting, SCCmec, agr and PFGE typing were done.Results:A total of 13 (41.9%) isolates were cefoxitin-resistant and mecA-positive, which were considered as MRSA. The SCCmec typing MRSA strains revealed type II in 1 (7.7%), type III in 9 (69.2%), and other types in 3 isolates (23.7%) cases. The agr typing of all 31 isolates showed that 14 (45.2%), 1 (3.2%), 6 (19.4%), and 10 (32.3%) strains belonged to agr groups 1, 3, 4, and unknown type, respectively. The pvl, tsst-1, hla-α, and psmα genes were positive in 3 (9.7%), 4 (12.9%), 21 (67.7%), and 31 (100%) isolates, respectively. Considering the cut-off values of ≥50%, 3 groups of related isolates (cluster A1, B1, and C1) in PFGE study were observed.Conclusion:The MRSA strains of this study were initially isolated as Community-associated S. aureus (CA-MRSA); however molecular characterization showed that a significant proportion of them had hospital-associated MRSA (HA-MRSA) features. Therefore, it is likely that the HA-MRSA strains are spread among the community.

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Antibiotic Resistance Pattern Among Staphylococcus aureus Isolated From Wound Cultures in Burn Patients: A Five-Year Study

Askarpour

Sedaghat

Hazrati

et al. 2020

Avicenna J Clin Microbiol Infect

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Background: Burn remains a globally significant life-threatening problem, especially in developing countries, and infection is considered as a major complication among burn patients. The rate of antibiotic-resistant bacteria isolated from burn patients has demonstrated a significant increase. In this regard, this study aimed to determine the antibiotic resistance pattern in Staphylococcus aureus isolated from patients’ burn wound infections. Methods: All available wound cultures of burn patients admitted to the burn unit of Emam-Reza hospital/ Mashhad, northeast Iran from March 2012 to March 2017 were included in this retrospective study. Then, the resistance of isolated S. aureus strains against 25 different antibiotic disks was studied based on the aim of the study. Results: Overall, 1973 patients were admitted, out of whom 4758 swab samples were taken from them. Out of 3188 micro-organisms isolated from burn wound cultures, 185 (5.8%) cases were S. aureus. Based on the results, the highest susceptibility rates were related to vancomycin (98.8%), cefazolin (72%), ciprofloxacin (75%), and gentamicin (74.6%). Conclusions: In general, vancomycin, cefazolin, and ciprofloxacin appeared to be the most effective agents among all tested antibiotics for S. aureus. The extensive use of antibiotics in treating infections has resulted in the emergence of resistant strains. Routine microbiological surveillance and careful in vitro testing before antibiotic use may help in the prevention of the ever-increasing antibiotic-resistant pathogens in burn infections.

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Detection of Integrons and Staphylococcal Cassette Chromosome ( SCCmec ) Types in Staphylococcus Aureus Isolated from Burn and Non-Burn Patients

Cited by 3 publications

References 21 publications

The First Report of Prevalence of Class 1-3 Integrons in Clinical Isolates of Staphylococcus aureus in Southwestern Iran: A Multicenter Study

The First Report of Prevalence of Class 1-3 Integrons in Clinical Isolates of Staphylococcus aureus in Southwestern Iran: A Multicenter Study

Molecular Characterization of Community-Associated Methicillin-Resistant Staphylococcus aureus in Iranian Burn Patients

Antibiotic Resistance Pattern Among Staphylococcus aureus Isolated From Wound Cultures in Burn Patients: A Five-Year Study

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