Detection of Infectious Bursal Disease Virus by Double Antibody Sandwich ELISA

Abstract

Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens. A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV from clinical samples. Two kinds of anti-IBDV antibodies, monoclonal antibody R63 and chicken anti-IBDV sera, were used for DAS-ELISA. Detection limit of IBDV by DAS-ELISA was approximately 10 2.7 EID 50 /ml. The DAS-ELISA detected IBDV from most (13/14) of vaccine products including mild, intermediate and intermediate-plus types. The DAS-ELISA also detected IBDV from all (19/19) of field Korean isolates including very virulent and intermediate-plus phenotypes. Our results indicate that the DAS-ELISA would provide useful diagnostic tool to detect IBDV from clinical samples as well as rapid quantitative detection of IBDV.

Rapid Detection of Infectious Bursal Disease Virus (IBDV) in Chickens by an Immunochromatographic Assay Kit

“…In this study, the detection limit of the IC kit was 10 3.1 EID50 per mL to 10 3.9 EID50 per mL. The detection limit of the IC kit was the same as double antigen capture ELISA (10 3.8 EID50/mL) but lower than RT-PCR (10 2.5 EID50/mL) according to the results by Jeon et al(2008)…”

“…Seven tissues including BF, cecal tonsil, spleen, kidney, proventriculus, liver and thymus were collected for all of infected and control birds. A 10% (spleen, kidney, proventriculus, liver and thymus) or 2% (BF and cecal tonsil) suspension of these tissue samples were applied for the IC kit (BioNote Inc., Korea) and double antigen sandwich (DAS)-ELISA (Jeon et al, 2008).…”

“…A double antigen sandwich ELISA for detection of IBDV (Jeon et al, 2008) To evaluate the applicability of the IC kit for the diagnosis of IBD, eight SPF chickens died within 4 days after very virulent IBDV (strain Kr/D62) infection were tested. Seven tissues including BF, cecal tonsil, spleen, kidney, proventriculus, liver and thymus were included for the testing.…”

Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4

“…For antigen quantification and to compare the antigenicity of the recombinant VP2 protein, we performed DAS-ELISA as described previously [ 39 ]. Briefly, MaxiSorp ELISA plates (Nunc) were coated with 50 μl of anti-VP2 monoclonal antibody (MAb) R63 [ 40 ] at the optimal concentration (4 μg/ml) in 0.01 M PBS at 37 °C for 1 h with constant shaking.…”

Current Trends on Surface Acoustic Wave Biosensors

“…In fact, a recent market study had predicted that the worldwide market value of biosensors will approach US$ 27 billion by 2022. [5] This growth has also been heavily influenced by recent technological advancement in the area of miniaturization further fueled by increasing demand for rapid and user-friendly point-of-care (POC) devices, not to mention the popularity of wearable biosensors in smart watches accessories currently available to the general populace.Ranging from classical enzyme-linked immunosorbent assay (ELISA) [6][7][8][9] to highly complex field effect transistors, [10][11][12] acoustic wave biosensors have interestingly remained as a peripheral scientific development despite their well-reported sensitivity. Acoustic waves, depending on their wave propagation characteristics, can be categorized as bulk acoustic wave (BAW), which travels throughout the bulk of piezoelectric materials, surface acoustic wave (SAW), which travels along the piezoelectric material surface, [13] and acoustic plate mode (APM) that travels via reflectance over many surfaces.…”