Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4

Lee, Hyun-Jeong; Kim, Ji-Ye; Kye, Soo-Jeong; Seul, Hee-Jung; Jung, Suk-Chan; Choi, Kang-Seuk

doi:10.1186/s12985-015-0403-4

Cited by 10 publications

(4 citation statements)

References 50 publications

(51 reference statements)

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“…Po względem strukturalnym VLP stanowi natywny wirusowy kapsyd pozbawiony kwasu nukleinowego. Badania z użyciem szczepionek VLP wykazały nawet 100% protekcję na zakażenie szczepem IBDV terenowym zarówno wysoce zjadliwym, klasycznym, jak i wariantem antygenowym (45,56,102).…”

Section: Badania Nad Nowymi Szczepionkamiunclassified

Selected aspects of infectious bursal disease – the current state of knowledge

Pikuła

Śmietanka

2023

Medycyna Weterynaryjna

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Infectious bursal disease (IBD) is a highly infectious and contagious immunosuppressive viral disease of chickens with a worldwide economic significance to the poultry industry. Over fifty years have passed since the first confirmed occurrence of the disease, and the virus has spread all over world and evolved into multiple genetic, antigenic and pathotypic variants, becoming a serious threat to the poultry industry. The primary tool in IBD eradication is the maintenance of strict biosecurity in poultry farms and implementation of vaccination programmes which should take into account the current epidemiological knowledge about the IBDV strains circulating in the field. This review article presents the current state of knowledge about the infectious bursal disease virus (IBDV) with special regard to the molecular biology of the virus, immunological aspects, as well as current and future prevention strategies.

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Section: Badania Nad Nowymi Szczepionkamiunclassified

Selected aspects of infectious bursal disease – the current state of knowledge

Pikuła

Śmietanka

2023

Medycyna Weterynaryjna

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“…It plays a role in the protease activity of VP4 (Wang et al, 2015b). In addition, VP4 also has an impact on the self-assembly of IBDV (Lee et al, 2015).…”

Section: Vp4mentioning

confidence: 99%

Molecular Biology, Immunosuppression and Pathogenesis of Infectious Bursal Disease Virus

Hussain¹,

Ashraf²,

Shamim³

et al. 2021

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Infectious bursal disease (IBD), caused by infectious bursal disease virus (IBDV), is one of the most devastating and immunosuppressive diseases of the poultry and has been a constraint on the sustainable food security around the globe including Pakistan. Poultry industry is the second biggest industry in Pakistan while IBD is an important disease seriously threatening poultry farming. Despite the use of mass and intense vaccination regimens, the disease continues to sustain in many countries around the globe including Pakistan. However, to some extent, the epidemic strains and epidemic patterns predominant strains of IBDV in Pakistan is unclear. This highlights the need to characterize field strains of IBDV to ascertain the evidence of vaccine failure and to establish foundations for the development of vaccine matching with the epidemic strains.

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“…The VP2 protein is a major host-protective antigen containing epitopes responsible for inducing neutralizing antibodies against IBDV [4]. The VP2 protein is the major structural component of the IBDV icosahedral capsid [5] and can spontaneously form particles that enhance immune protection [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Virus-like Particle Vaccines of Infectious Bursal Disease Virus Expressed in Escherichia coli Are Highly Immunogenic and Protect against Virulent Strain

Ji,

Li,

et al. 2023

Viruses

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Objectives: Infectious bursal disease virus (IBDV) is a highly contagious, acutely infectious agent that causes immunosuppression in chickens. We expressed IBDV VP2 proteins in Escherichia coli (E. coli) to develop an effective virus-like-particles (VLPs) vaccine and evaluated its immunogenicity. Methods: The VLPs produced in E. coli were used as an immunogen mixed with a water-in-mineral-oil adjuvant (MontanideTM ISA 71 VG, ISA 71 RVG) or a white oil (7#) adjuvant. VLPs without an adjuvant, commercial subunit vaccine, inactivated vaccine, and attenuated vaccine were used as controls. These test vaccines were intramuscularly injected into 19-day-old SPF chickens, which were challenged with the IBDV virulent strain at 30 days after vaccination. Results: The adjuvants boosted antibody production, and the adjuvant groups (except white oil) produced higher antibody levels than the non-adjuvanted controls and the commercial vaccine groups. In terms of cellular immunity, the VLPs plus adjuvant combinations produced higher levels of cytokines, IL-2, IL-4, and IFN-γ than the controls. Conclusion: IBDV VLPs plus the ISA 71 RVG adjuvant can be used as an optimal vaccine combination for improving the immune efficacy of IBD subunit vaccines, which can protect against the virulent strain.

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Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4

Cited by 10 publications

References 50 publications

Selected aspects of infectious bursal disease – the current state of knowledge

Selected aspects of infectious bursal disease – the current state of knowledge

Molecular Biology, Immunosuppression and Pathogenesis of Infectious Bursal Disease Virus

Virus-like Particle Vaccines of Infectious Bursal Disease Virus Expressed in Escherichia coli Are Highly Immunogenic and Protect against Virulent Strain

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