Detection of induced triploid scallop, Chlamys nobilis, by DNA microfluorometry with DAPI staining

Komaru, Akira; Uchimura, Yuushi; Ieyama, Hiroshi; Wada, Katsuhiko T.

doi:10.1016/0044-8486(88)90329-8

Cited by 57 publications

(17 citation statements)

References 12 publications

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“…The optimal procedure for triploidy induction and percentage survival at 3 h and at 18 days of culture was 31°C for 10 min and applied 10 min after insemination (meiosis I). The highest levels of triploidy (65.3-66.7 %) obtained with the highest temperature tested in the present study are comparable with those reported for other bivalve species, C. gigas (51-64 %, Quillet & Panelay 1986), T. semidecussatus (56 %, Gosling & Nolan 1989) and Mytilus galloprovincialis (81.2 %, Scarpa et al 1994), but less effective compared with chemical (CB) treatment in other species of scallops with percentage of triploids ranging from 66 to 94% in Argopecten irradians (Tabarini 1984), 88.2% in Chlamys nobilis (Komaru et al 1988); 78.5% in Chlamys varia (Baron et al 1989). According to Allen (1987) one reason for these differences in ploidy production is that heat shocks inhibit all development and only those eggs that were in a specifc stage of cell division will be affected by the high temperature-whereas CB does not appear to arrest development of eggs; so as they reach the vulnerable stage of cell divison they are affected by the chemical.…”

Section: Discussionsupporting

confidence: 76%

“…Heat has been used successfully to induce triploidy in Crassostrea gigas (Quillet & Panelay 1986;, Tapes semidecussatus (Gosling & Nolan 1989), Mytilus edulis Beaumont & Kelly 1989) and Mytilus galloprovincialis (Scarpa et al 1994). Among the species of scallops that have been induced by cytochalasin B (CB) treatment to produce triploids are Argopecten irradians (Tabarini 1984), Chamys nobilis (Komaru et al 1988), and Chlamys varia (Baron et al 1989). In this paper we present the results from two preliminary experiments on the induction of triploid embryos in A. purpuratus through the application of heat shocks, and investigate the appropriate combination of temperature, time after-insemination, and duration of the shock.…”

Section: Introductionmentioning

confidence: 99%

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Induction of triploid embryos by heat shock in the Chilean northern scallopArgopecten purpuratusLamarck, 1819

Toro

Sanhueza

Paredes

et al. 1995

New Zealand Journal of Marine and Freshwater Research

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Triploidy in embryos of the Chilean northern scallop, Argopecten purpuratus Lamarck, 1819, was induced with heat shock. Ploidy levels were assessed by chromosome counting after 3 h from embryos at the 8-16 cell stage of development. The efficiency of induction varied with the time of treatment post-insemination and the temperature of the shock. The duration of the shock showed a positive correlation with the percentage of triploids, the highest of which was obtained using 31°C for 15 min applied 10 min after insemination (66.7 %) but with low percentage of survival embryos after 3 h (26.4 %) and 18 days (11.2 %). The optimal procedure for triploidy induction (62.7 %) and survival after 3 h (62 %) and 18 days of culture (30.5 %) was the heat shock of 31°C for 10 min applied 10 min after fertilisation. We observed no significant difference in growth of shell length between controls and treated larvae after 18 days of culture.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Induction of triploid embryos by heat shock in the Chilean northern scallopArgopecten purpuratusLamarck, 1819

Toro

Sanhueza

Paredes

et al. 1995

New Zealand Journal of Marine and Freshwater Research

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“…Sixmicron serial sections were cut and stained with Meyer's hematoxylin and eosin. Eggs released from the exhalent siphon were fixed with 10% formaldehyde, rinsed with distilled water twice, then stained with 4, 6-diamidino-2-phenylindole (DAPI) solution (Komaru et al 1988) and observed with a fluorescence microscope. Cytological evidence of spontaneous androgenesis in the freshwater clam Corbicula leana Prime gill of the parents were compared by microfluorometry with DAPI staining (Komaru et al 1988).…”

Section: Light and Fluorescence Microscopymentioning

confidence: 99%

Cytological evidence of spontaneous androgenesis in the freshwater clam Corbicula leana Prime

Komaru

Kawagishi

Konishi

1998

Development Genes and Evolution

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Cytological observations and DNA microfluorometry of the hermaphrodite freshwater triploid clam Corbicula leana revealed unusual androgenetic development as follows: (1) the maternal genome of zygotes was extruded as two polar bodies just after karyokinesis of the first meiosis, (2) only chromosomes derived from one male pronucleus constituted the metaphase of the first cleavage of zygotes, (3) DNA content of 7-day-old veliger larvae was identical to the somatic cells of the parent. This spontaneous androgenetic process in C. leana zygotes is the first case in the phylum Mollusca and may be related to the specialized mode of reproduction; i.e. hermaphroditism and self-fertilization.

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“…These studies have shown that the triploid condition induced by inhibiting the release of the second polar body in fertilized eggs, and therefore the conclusion of meiosis II, induces not only functional sterility, but also structural sterility as practically no gametes of either sex are produced in adult triploids [11]. This is not the case for all triploid mollusks, but complete or structural sterility has been found for some triploid-induced species [12]–[14] whereas other species, including other scallops, are capable of producing gametes [15]–[17], even if they are aneuploids [18]. However, it is not known if tolerance to aneuploidy causes the differences in triploid sterility between species in other taxa, and other mechanisms might be involved.…”

Section: Introductionmentioning

confidence: 99%

Identification of Male Gametogenesis Expressed Genes from the Scallop Nodipecten subnodosus by Suppressive Subtraction Hybridization and Pyrosequencing

et al. 2013

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Despite the great advances in sequencing technologies, genomic and transcriptomic information for marine non-model species with ecological, evolutionary, and economical interest is still scarce. In this work we aimed to identify genes expressed during spermatogenesis in the functional hermaphrodite scallop Nodipecten subnodosus (Mollusca: Bivalvia: Pectinidae), with the purpose of obtaining a panel of genes that would allow for the study of differentially transcribed genes between diploid and triploid scallops in the context of meiotic arrest and reproductive sterility. Because our aim was to isolate genes involved in meiosis and other testis maturation-related processes, we generated suppressive subtractive hybridization libraries of testis vs. inactive gonad. We obtained 352 and 177 ESTs by clone sequencing, and using pyrosequencing (454-Roche) we maximized the identified ESTs to 34,276 reads. A total of 1,153 genes from the testis library had a blastx hit and GO annotation, including genes specific for meiosis, spermatogenesis, sex-differentiation, and transposable elements. Some of the identified meiosis genes function in chromosome pairing (scp2, scp3), recombination and DNA repair (dmc1, rad51, ccnb1ip1/hei10), and meiotic checkpoints (rad1, hormad1, dtl/cdt2). Gene expression analyses in different gametogenic stages in both sexual regions of the gonad of meiosis genes confirmed that the expression was specific or increased towards the maturing testis. Spermatogenesis genes included known testis-specific ones (kelch-10, shippo1, adad1), with some of these known to be associated to sterility. Sex differentiation genes included one of the most conserved genes at the bottom of the sex-determination cascade (dmrt1). Transcript from transposable elements, reverse transcriptase, and transposases in this library evidenced that transposition is an active process during spermatogenesis in N. subnodosus. In relation to the inactive library, we identified 833 transcripts with functional annotation related to activation of the transcription and translation machinery, as well as to germline control and maintenance.

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Detection of induced triploid scallop, Chlamys nobilis, by DNA microfluorometry with DAPI staining

Cited by 57 publications

References 12 publications

Induction of triploid embryos by heat shock in the Chilean northern scallopArgopecten purpuratusLamarck, 1819

Induction of triploid embryos by heat shock in the Chilean northern scallopArgopecten purpuratusLamarck, 1819

Cytological evidence of spontaneous androgenesis in the freshwater clam Corbicula leana Prime

Identification of Male Gametogenesis Expressed Genes from the Scallop Nodipecten subnodosus by Suppressive Subtraction Hybridization and Pyrosequencing

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