Detection of Individual Microbubbles of an Ultrasound Contrast Agent

Klibanov, Alexander L.; Rasche, Peter T; Hughes, Michael S.; Wojdyla, Jolette K.; Galen, Karen P.; Wible, James H.; Brandenburger, Gary H.

doi:10.1016/s1076-6332(03)80203-9

Cited by 53 publications

(28 citation statements)

References 4 publications

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“…The backscatter by a bubble is proportional to the sixth power of its diameter; the effectiveness of echo response is also dependent on the ultrasound frequency. 17 It was recently shown that smaller bubbles indeed do not present themselves as efficient scatterers of ultrasound and cannot be detected at all unless the imaging is performed at high MI, 11 and we intentionally avoided high MI in this study to minimize reduction of microbubble concentration during imaging. A second probable explanation could reside with issue of the interrogated volume calculation.…”

Section: Klibanov Et Almentioning

confidence: 99%

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Detection of Individual Microbubbles of Ultrasound Contrast Agents

Klibanov

Rasche²,

Hughes³

et al. 2004

Investigative Radiology

187

112

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Section: Klibanov Et Almentioning

confidence: 99%

“…Recently, it was reported that very dilute dispersions of microbubbles 11,12 do present individual foci of ultrasound scatterers that might be consistent with the individual microbubbles responsible; however, direct microbubble counting comparison has not been reported. Such independent counting of microbubbles could be performed by Coulter counting.…”

mentioning

confidence: 99%

Detection of Individual Microbubbles of Ultrasound Contrast Agents

Klibanov

Rasche²,

Hughes³

et al. 2004

Investigative Radiology

187

112

View full text Add to dashboard Cite

show abstract

“…Data were recorded on a 1.25-cm videotape using an S-VHS recorder. Phospholipid-coated perfluorocarbon gas microbubbles were manufactured at the University of Virginia as reported previously (24). Microbubble concentration and size distribution were determined by electrozone sensing with a Coulter Multisizer IIe (Beckman-Coulter).…”

mentioning

confidence: 99%

Microvascular Recruitment Is an Early Insulin Effect That Regulates Skeletal Muscle Glucose Uptake In Vivo

et al. 2004

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Insulin increases glucose disposal into muscle. In addition, in vivo insulin elicits distinct nitric oxide synthase-dependent vascular responses to increase total skeletal muscle blood flow and to recruit muscle capillaries (by relaxing resistance and terminal arterioles, respectively). In the current study, we compared the temporal sequence of vascular and metabolic responses to a 30-min physiological infusion of insulin (3 mU ⅐ min ؊1 ⅐ kg ؊1 , euglycemic clamp) or saline in rat skeletal muscle in vivo. We used contrast-enhanced ultrasound to continuously quantify microvascular volume. Insulin recruited microvasculature within 5-10 min (P < 0.05), and this preceded both activation of insulin-signaling pathways and increases in glucose disposal in muscle, as well as changes in total leg blood flow. Moreover, L-NAME (N -nitro-L-arginine-methyl ester), a specific inhibitor of nitric oxide synthase, blocked this early microvascular recruitment (P < 0.05) and at least partially inhibited early increases in muscle glucose uptake (P < 0.05). We conclude that insulin rapidly recruits skeletal muscle capillaries in vivo by a nitric oxidedependent action, and the increase in capillary recruitment may contribute to the subsequent glucose uptake.

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“…They have a typical size of 2–6 μm, and can circulate in the blood and pass through CNS capillaries. Since microbubbles contain a gas–liquid interface that is highly responsive to ultrasound mechanical stress, microbubbles provide strong ultrasound energy scattering that enables microbubbles to be applied in diagnostic ultrasound as a contrast agent [21–23]. In addition, ultrasound energy interactions with microbubbles have been confirmed to transiently permeate CNS capillaries [24–26].…”

Section: Introductionmentioning

confidence: 99%

Drug-carrying microbubbles as a theranostic tool in convection-enhanced delivery for brain tumor therapy

et al. 2017

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Convection-enhanced delivery (CED) is a promising technique for infusing a therapeutic agent through a catheter with a pressure gradient to create bulk flow for improving drug spread into the brain. So far, gadopentetate dimeglumine (Gd-DTPA) is the most commonly applied surrogate agent for predicting drug distribution through magnetic resonance imaging (MRI). However, Gd-DTPA provides only a short observation duration, and concurrent infusion provides an indirect measure of the exact drug distribution. In this study, we propose using microbubbles as a contrast agent for MRI monitoring, and evaluate their use as a drug-carrying vehicle to directly monitor the infused drug. Results show that microbubbles can provide excellent detectability through MRI relaxometry and accurately represent drug distribution during CED infusion. Compared with the short half-life of Gd-DTPA (1-2 hours), microbubbles allow an extended observation period of up to 12 hours. Moreover, microbubbles provide a sufficiently high drug payload, and glioma mice that underwent a CED infusion of microbubbles carrying doxorubicin presented considerable tumor growth suppression and a significantly improved survival rate. This study recommends microbubbles as a new theranostic tool for CED procedures.

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Detection of Individual Microbubbles of an Ultrasound Contrast Agent

Cited by 53 publications

References 4 publications

Detection of Individual Microbubbles of Ultrasound Contrast Agents

Detection of Individual Microbubbles of Ultrasound Contrast Agents

Microvascular Recruitment Is an Early Insulin Effect That Regulates Skeletal Muscle Glucose Uptake In Vivo

Drug-carrying microbubbles as a theranostic tool in convection-enhanced delivery for brain tumor therapy

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