Detection of immunogenic proteins from Anopheles sundaicussalivary glands in the human serum

Armiyanti, Yunita; Nuryady, Mohammad Mirza; Arifianto, Renam Putra; Nurmariana, Elisa; Senjarini, Kartika; Fitri, Loeki Enggar; Sardjono, Teguh Wahju

doi:10.1590/0037-8682-0185-2015

Cited by 10 publications

(7 citation statements)

References 29 publications

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“…The cross-reactivity analysis showed that specific IgY antibodies develop against salivary proteins from malariatransmitting mosquitoes. These results corroborate the findings of Armiyanti et al (2015), who used salivary gland extracts from Anopheles sundaicus to detect the IgG antibodies production in serum samples from individuals living in malaria-endemic areas. Armiyanti et al identified sixteen proteins that induce the production of IgG antibodies in hosts: these varied from 22 to 144 kDa, with protein bands of 46, 41, 33 and 31 kDa being the most immunogenic.…”

supporting

confidence: 89%

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Chicken eggs as a surveillance tool for malaria and leishmaniasis vector presence

Silva

Almeida

Marialva

et al. 2019

Rev. Soc. Bras. Med. Trop.

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Introduction: Malaria and leishmaniases are transmitted by vectors during blood-feeding. Vector-infected animals develop antibodies against the vector's saliva. This study evaluated IgY antibody detection in the chicken eggs exposed to bites from Migonemyia migonei, Lutzomyia longipalpis and Anopheles aquasalis. Methods: We used ELISA to quantify the antibody levels in the sera and exposed chicken eggs. Results: High IgY levels were observed following immunization; furthermore, higher reactivity was observed in the eggs and species-specific immune response was observed post final immunization. Conclusions: Chicken eggs can be used as sentinels to surveil vector saliva antibodies.

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supporting

confidence: 89%

“…Armiyanti et al identified sixteen proteins that induce the production of IgG antibodies in hosts: these varied from 22 to 144 kDa, with protein bands of 46, 41, 33 and 31 kDa being the most immunogenic. This demonstrates that antibodies can be developed against the salivary proteins of specific species 13 .…”

mentioning

confidence: 87%

Chicken eggs as a surveillance tool for malaria and leishmaniasis vector presence

Silva

Almeida

Marialva

et al. 2019

Rev. Soc. Bras. Med. Trop.

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“…It is tempting to postulate that APCs could be overwhelmed with mosquito-derived peptides, leading to mosquito-specific responses that self-regulate antigen-presenting DCs even as these APCs simultaneously present smaller quantities of parasite-derived antigens. Mosquito-derived antigens are known to induce antibody responses (54)(55)(56)(57), and both saliva and sporozoites remain in the skin for up to 18 h postbite (58).…”

Section: Figmentioning

confidence: 99%

Purification of Plasmodium Sporozoites Enhances Parasite-Specific CD8 ⁺ T Cell Responses

2016

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cMalaria infection caused by Plasmodium parasites continues to cause enormous morbidity and mortality in areas where it is endemic, and there is no licensed vaccine capable of inducing sterile protection. Hyperimmunization with attenuated whole sporozoites can induce sterile protective immune responses targeting preerythrocytic antigens. Most animal models of hyperimmunization rely on sporozoites dissected from mosquito salivary glands and injected without further purification. In BALB/c mice, repeated small doses of P. yoelii sporozoites progressively expand the population of sporozoite-specific CD8 ؉ T cells. In this study, large secondary doses of unpurified sporozoites unexpectedly led to contraction of sporozoite-specific CD8 ؉ T cell responses in sporozoite-primed mice. While sporozoite-primed CD8 ؉ T cells alternatively can be expanded by secondary exposure to Listeria monocytogenes expressing recombinant Plasmodium antigens, such expansion was potently inhibited by coinjection of large doses of unpurified sporozoites and by uninfected salivary glands alone. Purification of sporozoites away from mosquito salivary gland debris by density gradient centrifugation eliminated salivary gland-associated inhibition. Thus, the inhibitory effect appears to be due to exposure to uninfected mosquito salivary glands rather than sporozoites. To further assess the effect of salivary gland exposure on later sporozoite vaccinations, mice were immunized with uninfected salivary glands from a single mosquito. Compared to naive mice, salivary gland presensitization reduced subsequent liver burdens by 71%. These data show that a component(s) in mosquito salivary glands reduces liver infection, thereby limiting antigen dose and contributing to lower-magnitude T cell responses. These findings suggest that sporozoite immunogenicity studies be performed using purified sporozoites whenever feasible.

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“…New approaches for entomological malaria transmission metrics are needed for elimination, particularly in regions with diverse vectors and low parasite densities 31 . Identifying P. vivax or vector-specific biomarkers in mosquito saliva would improve estimates of transmission variables, and such biomarkers may also serve as targets for transmission interruption 32 , 33 .…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host

Roth

Adapa

Zhang

et al. 2018

Sci Rep

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Malaria parasites transmitted by mosquito bite are remarkably efficient in establishing human infections. The infection process requires roughly 30 minutes and is highly complex as quiescent sporozoites injected with mosquito saliva must be rapidly activated in the skin, migrate through the body, and infect the liver. This process is poorly understood for Plasmodium vivax due to low infectivity in the in vitro models. To study this skin-to-liver-stage of malaria, we used quantitative bioassays coupled with transcriptomics to evaluate parasite changes linked with mammalian microenvironmental factors. Our in vitro phenotyping and RNA-seq analyses revealed key microenvironmental relationships with distinct biological functions. Most notable, preservation of sporozoite quiescence by exposure to insect-like factors coupled with strategic activation limits untimely activation of invasion-associated genes to dramatically increase hepatocyte invasion rates. We also report the first transcriptomic analysis of the P. vivax sporozoite interaction in salivary glands identifying 118 infection-related differentially-regulated Anopheles dirus genes. These results provide important new insights in malaria parasite biology and identify priority targets for antimalarial therapeutic interventions to block P. vivax infection.

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Detection of immunogenic proteins from Anopheles sundaicussalivary glands in the human serum

Cited by 10 publications

References 29 publications

Chicken eggs as a surveillance tool for malaria and leishmaniasis vector presence

Chicken eggs as a surveillance tool for malaria and leishmaniasis vector presence

Purification of Plasmodium Sporozoites Enhances Parasite-Specific CD8 ⁺ T Cell Responses

Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host

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Detection of immunogenic proteins from Anopheles sundaicussalivary glands in the human serum

Cited by 10 publications

References 29 publications

Chicken eggs as a surveillance tool for malaria and leishmaniasis vector presence

Chicken eggs as a surveillance tool for malaria and leishmaniasis vector presence

Purification of Plasmodium Sporozoites Enhances Parasite-Specific CD8 + T Cell Responses

Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host

Contact Info

Product

Resources

About

Purification of Plasmodium Sporozoites Enhances Parasite-Specific CD8 ⁺ T Cell Responses