Detection ofToxoplasma gondiiin Raw Caprine, Ovine, Buffalo, Bovine, and Camel Milk Using Cell Cultivation, Cat Bioassay, Capture ELISA, and PCR Methods in Iran

Abstract

This study was conducted to determine the presence of Toxoplasma gondii in animal milk samples in Iran. From a total of 395 dairy herds in three provinces of Iran, 66 bovine, 58 ovine, 54 caprine, 33 buffalo, and 30 camel herds were studied, and from these parts of Iran, 200 bovine, 185 ovine, 180 caprine, 164 buffalo, and 160 camel milk samples were collected from various seasons. Samples were tested for Toxoplasma gondii by cell line culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) technique. Only the results of cell line cultivation were confirmed by bioassay in cat. Results indicated that all herds were infected with Toxoplasma gondii. The culture method showed that 51 out of 889 milk samples (5.73%) were positive for Toxoplasma gondii, and all 51 positive culture results were positive with bioassay in cat. The Fars province had the highest prevalence of Toxoplasma gondii (6.84%). The ELISA test showed that 41 milk samples (4.61%) were positive for the presence of Toxoplasma gondii, while the PCR showed that 46 milk samples were positive for Toxoplasma gondii. The results showed higher sensitivity of PCR and higher specificity of ELISA. Caprine had the highest (10%) and camel had the lowest (3.12%) prevalence rate of parasite. The summer season had the highest (76.47%) but winter (3.92) had the lowest incidence of Toxoplasma gondii. This study is the first prevalence report of direct detection of Toxoplasma gondii in animal milk samples in Iran.

A preliminary study on the quality and safety of milk in donkeys positive for Toxoplasma gondii

“…As is well known, the data concerning the excretion of parasitic DNA in milk does not indicate the presence of live forms. Tachyzoite stages of T. gondii have also been found in milk of several species, including sheep, goats, camels, buffalos and cows; and infection in humans due to the ingestion of raw goat's milk has been documented (Dehkordi et al, 2013). In addition, tachyzoite penetration through the oral-pharyngeal mucosa has been demonstrated in cats.…”

Knowledge, Attitude and Practice of Community Towards Zoonotic Importance of <i>Toxoplasma</i> Infection in Central Afar Region, North East Ethiopia

“…Few other studies investigated the detection of natural infection of the parasite within milk from camel and donkeys in which tachyzoites found in the milk are attributed for transmission of the disease. In Iran, scientists reported a rate of camel milk contamination of 3.12% by culture bioassays [25].…”

Seroprevalence of Toxoplasma gondii infection in water buffaloes (Bubalus bubalis) in Veracruz State, Mexico and its association with climatic factors

“…In general, buffaloes, like cattle, are considered resistant to clinical toxoplasmosis and there is no valid report of isolation of viable T. gondii from buffalo meat [1,3]. Recently, Dehkordi et al [4] reported isolation of viable T. gondii from milk of 7 of 164 buffaloes in Iran, but the validity of these results has been questioned [5]. This report has raised concerns regarding transmission of T. gondii by consuming raw products from buffaloes.…”